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Article: Ki-67 antigen expression in hepatocellular carcinoma using monoclonal antibody MIB1. A comparison with proliferating cell nuclear antigen

TitleKi-67 antigen expression in hepatocellular carcinoma using monoclonal antibody MIB1. A comparison with proliferating cell nuclear antigen
Authors
KeywordsCellular differentiation
Encapsulation
Hepatocellular carcinoma
MIB1
Prognosis
Issue Date1995
PublisherAmerican Society for Clinical Pathology. The Journal's web site is located at http://www.ajcp.com
Citation
American Journal of Clinical Pathology, 1995, v. 104 n. 3, p. 313-318 How to Cite?
AbstractTo evaluate the prognostic significance and clinicopathologic correlation of proliferative activity in patients with hepatocellular carcinoma, Ki-67 antigen expression was examined using immunohistochemical staining with monoclonal antibody MIB1. Seventy-two patients (65 men, 7 women; age range 24-77 years, mean, 52 years) having hepatocellular carcinoma surgically resected were studied. Tumor and nontumorous tissues were stained with monoclonal antibody MIB1 with microwave oven pretreatment. Tumor and nontumor MIB1 (T-MIB1 and NT-MIB1) scores were assessed by counting the positive staining nuclei per 1,000 cells. The T-MIB1 score ranged from 5-630 per 1,000 cells (mean ± standard deviation [SD] = 145 ± 162). It was found to be significantly higher in less well-differentiated tumors (Edmondson's grades III and IV) than in well-differentiated ones (Edmondson's grades I and II) (P = .017). The T-MIB1 score was also higher in non-encapsulated tumors than in encapsulated ones, although it did not reach statistical significance (P = .069). It had no influence on tumor size, tumor invasiveness, the background disease in the nontumorous livers, patient's HBsAg status, or serum α- fetoprotein levels. Diseases in the nontumorous livers or patients' HBsAg status had no influence on the NT-MIB1 scores. When the tumors were stratified into two groups with T-MIB1 score ≤20 and T-MIB1 score >20, those patients with score ≤20 had significantly longer disease-free survival (DFS) than those with scores > 29 (median DFS: 34 months and 4.7 months, respectively; P = .011). In addition, MIB1 and PCNA were closely correlated (P < .01). The authors conclude that proliferative activity in hepatocellular carcinoma, as defined by MIB1 immunohistochemical analysis, is significantly related to tumor cellular differentiation. It is also a potentially valuable prognostic factor in patients with this tumor.
Persistent Identifierhttp://hdl.handle.net/10722/148048
ISSN
2015 Impact Factor: 2.278
2015 SCImago Journal Rankings: 1.129
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNg, IOLen_HK
dc.contributor.authorNa, Jen_HK
dc.contributor.authorLai, ECSen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorNg, Men_HK
dc.date.accessioned2012-05-29T06:10:34Z-
dc.date.available2012-05-29T06:10:34Z-
dc.date.issued1995en_HK
dc.identifier.citationAmerican Journal of Clinical Pathology, 1995, v. 104 n. 3, p. 313-318en_HK
dc.identifier.issn0002-9173en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148048-
dc.description.abstractTo evaluate the prognostic significance and clinicopathologic correlation of proliferative activity in patients with hepatocellular carcinoma, Ki-67 antigen expression was examined using immunohistochemical staining with monoclonal antibody MIB1. Seventy-two patients (65 men, 7 women; age range 24-77 years, mean, 52 years) having hepatocellular carcinoma surgically resected were studied. Tumor and nontumorous tissues were stained with monoclonal antibody MIB1 with microwave oven pretreatment. Tumor and nontumor MIB1 (T-MIB1 and NT-MIB1) scores were assessed by counting the positive staining nuclei per 1,000 cells. The T-MIB1 score ranged from 5-630 per 1,000 cells (mean ± standard deviation [SD] = 145 ± 162). It was found to be significantly higher in less well-differentiated tumors (Edmondson's grades III and IV) than in well-differentiated ones (Edmondson's grades I and II) (P = .017). The T-MIB1 score was also higher in non-encapsulated tumors than in encapsulated ones, although it did not reach statistical significance (P = .069). It had no influence on tumor size, tumor invasiveness, the background disease in the nontumorous livers, patient's HBsAg status, or serum α- fetoprotein levels. Diseases in the nontumorous livers or patients' HBsAg status had no influence on the NT-MIB1 scores. When the tumors were stratified into two groups with T-MIB1 score ≤20 and T-MIB1 score >20, those patients with score ≤20 had significantly longer disease-free survival (DFS) than those with scores > 29 (median DFS: 34 months and 4.7 months, respectively; P = .011). In addition, MIB1 and PCNA were closely correlated (P < .01). The authors conclude that proliferative activity in hepatocellular carcinoma, as defined by MIB1 immunohistochemical analysis, is significantly related to tumor cellular differentiation. It is also a potentially valuable prognostic factor in patients with this tumor.en_HK
dc.languageengen_US
dc.publisherAmerican Society for Clinical Pathology. The Journal's web site is located at http://www.ajcp.comen_HK
dc.relation.ispartofAmerican Journal of Clinical Pathologyen_HK
dc.subjectCellular differentiationen_HK
dc.subjectEncapsulationen_HK
dc.subjectHepatocellular carcinomaen_HK
dc.subjectMIB1en_HK
dc.subjectPrognosisen_HK
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAntibodies, Monoclonal - Diagnostic Useen_US
dc.subject.meshCarcinoma, Hepatocellular - Immunology - Mortality - Pathologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistry - Methodsen_US
dc.subject.meshKi-67 Antigenen_US
dc.subject.meshLiver Neoplasms - Immunology - Mortality - Pathologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNeoplasm Proteins - Analysisen_US
dc.subject.meshNuclear Proteins - Analysisen_US
dc.subject.meshPrognosisen_US
dc.subject.meshProliferating Cell Nuclear Antigen - Analysisen_US
dc.subject.meshStaining And Labelingen_US
dc.subject.meshSurvival Analysisen_US
dc.titleKi-67 antigen expression in hepatocellular carcinoma using monoclonal antibody MIB1. A comparison with proliferating cell nuclear antigenen_HK
dc.typeArticleen_HK
dc.identifier.emailNg, IOL: iolng@hkucc.hku.hken_HK
dc.identifier.emailNg, M: hrmeman@HKUCC.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hk-
dc.identifier.authorityNg, IOL=rp00335en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7545866-
dc.identifier.scopuseid_2-s2.0-0029124870en_HK
dc.identifier.hkuros8758-
dc.identifier.volume104en_HK
dc.identifier.issue3en_HK
dc.identifier.spage313en_HK
dc.identifier.epage318en_HK
dc.identifier.isiWOS:A1995RR99000014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridNg, IOL=7102753722en_HK
dc.identifier.scopusauthoridNa, J=7103258608en_HK
dc.identifier.scopusauthoridLai, ECS=55187376900en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK
dc.identifier.scopusauthoridNg, M=7202076310en_HK
dc.customcontrol.immutablesml 130703-

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