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Article: Prognostic significance of proliferating cell nuclear antigen expression in hepatocellular carcinoma

TitlePrognostic significance of proliferating cell nuclear antigen expression in hepatocellular carcinoma
Authors
Keywordshepatocellular carcinoma
immunohistochemistry
prognosis
proliferating cell nuclear antigen
Issue Date1994
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741
Citation
Cancer, 1994, v. 73 n. 9, p. 2268-2274 How to Cite?
AbstractBackground. Proliferating cell nuclear antigen (PCNA) is a nuclear protein synthesized in G 1/S-phase of the cell cycle and therefore is related to cell proliferative activity. In an attempt to evaluate its prognostic significance and clinicopathologic correlation in patients with hepatocellular carcinoma (HCC), the proliferative activity was studied using immunohistochemical staining with monoclonal antibody to PCNA. Methods. Seventy-two patients (64 men, 8 women; mean age, 52 years [range, 24-77 years]) having HCC surgically resected were studied. Tumor and nontumor tissues were selected from each case and stained with PCNA antibody. Tumor and nontumor PCNA (T-PCNA and NT- PCNA) scores were assessed by counting the positive staining nuclei per 1000 cells. Results. The T-PCNA score ranged from 10 to 894 per 1000 cells (mean ± standard deviation = 333 ± 263). It was found to be significantly and positively associated with positive margin (P = 0.003), direct liver invasion by tumor (P = 0.021), and venous permeation (P = 0.020), features directly or indirectly related to tumor invasiveness. However, it had no significant association with tumor size, cellular differentiation, or patients' hepatitis B surface antigen (HBsAg) status. When the tumors were stratified into two groups with a T-PCNA score less than or equal to 200 and a T-PCNA score greater than 200, those patients with a T-PCNA score less than or equal to 200 had significantly longer disease-free survival (DFS) and actual survival (AS) rates than those with scores greater than 200 (median DFS and AS, 34.6 and 49.3 months and 7.7 and 19.1 months, respectively; P = 0.019 and 0.035 for DFS and AS, respectively). T-PCNA and NT-PCNA scores had no significant correlation with the HBsAg status of the patients. Conclusions. Proliferative activity in HCC, as defined by PCNA immunohistochemical analysis, is significantly related to tumor invasiveness. It is also a potentially valuable prognostic factor in patients with this tumor.
Persistent Identifierhttp://hdl.handle.net/10722/148020
ISSN
2015 Impact Factor: 5.649
2015 SCImago Journal Rankings: 3.188
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNg, IOLen_HK
dc.contributor.authorLai, ECSen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorNg, Men_HK
dc.contributor.authorChan, ASYen_HK
dc.contributor.authorSo, MKPen_HK
dc.date.accessioned2012-05-29T06:10:25Z-
dc.date.available2012-05-29T06:10:25Z-
dc.date.issued1994en_HK
dc.identifier.citationCancer, 1994, v. 73 n. 9, p. 2268-2274en_HK
dc.identifier.issn0008-543Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/148020-
dc.description.abstractBackground. Proliferating cell nuclear antigen (PCNA) is a nuclear protein synthesized in G 1/S-phase of the cell cycle and therefore is related to cell proliferative activity. In an attempt to evaluate its prognostic significance and clinicopathologic correlation in patients with hepatocellular carcinoma (HCC), the proliferative activity was studied using immunohistochemical staining with monoclonal antibody to PCNA. Methods. Seventy-two patients (64 men, 8 women; mean age, 52 years [range, 24-77 years]) having HCC surgically resected were studied. Tumor and nontumor tissues were selected from each case and stained with PCNA antibody. Tumor and nontumor PCNA (T-PCNA and NT- PCNA) scores were assessed by counting the positive staining nuclei per 1000 cells. Results. The T-PCNA score ranged from 10 to 894 per 1000 cells (mean ± standard deviation = 333 ± 263). It was found to be significantly and positively associated with positive margin (P = 0.003), direct liver invasion by tumor (P = 0.021), and venous permeation (P = 0.020), features directly or indirectly related to tumor invasiveness. However, it had no significant association with tumor size, cellular differentiation, or patients' hepatitis B surface antigen (HBsAg) status. When the tumors were stratified into two groups with a T-PCNA score less than or equal to 200 and a T-PCNA score greater than 200, those patients with a T-PCNA score less than or equal to 200 had significantly longer disease-free survival (DFS) and actual survival (AS) rates than those with scores greater than 200 (median DFS and AS, 34.6 and 49.3 months and 7.7 and 19.1 months, respectively; P = 0.019 and 0.035 for DFS and AS, respectively). T-PCNA and NT-PCNA scores had no significant correlation with the HBsAg status of the patients. Conclusions. Proliferative activity in HCC, as defined by PCNA immunohistochemical analysis, is significantly related to tumor invasiveness. It is also a potentially valuable prognostic factor in patients with this tumor.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741en_HK
dc.relation.ispartofCanceren_HK
dc.subjecthepatocellular carcinomaen_HK
dc.subjectimmunohistochemistryen_HK
dc.subjectprognosisen_HK
dc.subjectproliferating cell nuclear antigenen_HK
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAntigens, Neoplasm - Analysis - Geneticsen_US
dc.subject.meshCarcinoma, Hepatocellular - Genetics - Immunology - Pathologyen_US
dc.subject.meshCell Divisionen_US
dc.subject.meshCell Nucleus - Immunology - Ultrastructureen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Regulation, Neoplasticen_US
dc.subject.meshHepatitis B Surface Antigens - Analysisen_US
dc.subject.meshHumansen_US
dc.subject.meshLiver Cirrhosis - Pathologyen_US
dc.subject.meshLiver Neoplasms - Genetics - Immunology - Pathologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNeoplasm Invasivenessen_US
dc.subject.meshNuclear Proteins - Analysis - Geneticsen_US
dc.subject.meshPrognosisen_US
dc.subject.meshProliferating Cell Nuclear Antigenen_US
dc.subject.meshRisk Factorsen_US
dc.subject.meshStaining And Labelingen_US
dc.subject.meshSurvival Rateen_US
dc.titlePrognostic significance of proliferating cell nuclear antigen expression in hepatocellular carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.emailNg, IOL: iolng@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.authorityNg, IOL=rp00335en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7513246-
dc.identifier.scopuseid_2-s2.0-0028271583en_HK
dc.identifier.volume73en_HK
dc.identifier.issue9en_HK
dc.identifier.spage2268en_HK
dc.identifier.epage2274en_HK
dc.identifier.isiWOS:A1994NH42900005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridNg, IOL=7102753722en_HK
dc.identifier.scopusauthoridLai, ECS=55187433300en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK
dc.identifier.scopusauthoridNg, M=7202076310en_HK
dc.identifier.scopusauthoridChan, ASY=7403168075en_HK
dc.identifier.scopusauthoridSo, MKP=36719004700en_HK

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