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Article: Flow cytometric analysis of DNA ploidy in hepatocellular carcinoma

TitleFlow cytometric analysis of DNA ploidy in hepatocellular carcinoma
Authors
Issue Date1994
PublisherAmerican Society for Clinical Pathology. The Journal's web site is located at http://www.ajcp.com
Citation
American Journal Of Clinical Pathology, 1994, v. 102 n. 1, p. 80-86 How to Cite?
AbstractTo examine the prognostic and pathobiologic significance of DNA content, the authors studied the surgically resected hepatocellular carcinomas of 69 patients by flow cytometric analysis. Homogeneity of DNA content within individual tumor nodules was present in 15 (88%) of 17 specimens examined. Similarly, homogeneity of DNA content in tumors having multiple nodules was found in 8 (73%) of 11 specimens. In 64 tumors with homogeneous DNA content evaluated further, DNA aneuploidy was present in 30 (46.9%) specimens, and the proportion of aneuploid tumors was similar in the large (> 5 cm in diameter, n = 35) and small (≤ 5 cm, n = 25) lesions, at 42.9% and 40%, respectively. Overall, the diploid tumors had serum α-fetoprotein levels increased to greater than 500 μg/mL more frequently than did the aneuploid tumors (P = .037). DNA content did not correlate significantly with hepatitis B surface antigen, presence of liver cirrhosis, cellular differentiation, tumor size, or tumor encapsulation. DNA content also did not influence tumor invasiveness in terms of liver invasion, presence of tumor microsatellites, or venous permeation. With multivariate Cox regression analysis, tumor encapsulation (P = .015), negative resection margin (P = .007), and DNA ploidy pattern stratified according to large and small tumors (P = .024) were favorable prognostic factors. In the small tumors, a diploid DNA pattern was associated with significantly better patient survival than was an aneuploid pattern (P = .012). In the large tumors, on the contrary, a diploid pattern was associated with poorer patient survival than was an aneuploid pattern (P = .029). The authors conclude that DNA ploidy pattern in hepatocellular carcinomas is homogeneous and stable. It supplements other predictors in prognostication when the lesions are stratified into small and large ones by tumor size. It is of particular importance as a predictor because it can be assessed preoperatively in needle-biopsy specimens.
Persistent Identifierhttp://hdl.handle.net/10722/148013
ISSN
2015 Impact Factor: 2.278
2015 SCImago Journal Rankings: 1.129
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNg, IOLen_US
dc.contributor.authorLai, ECSen_US
dc.contributor.authorHo, JCWen_US
dc.contributor.authorCheung, LKNen_US
dc.contributor.authorNg, MMTen_US
dc.contributor.authorSo, MKPen_US
dc.date.accessioned2012-05-29T06:10:22Z-
dc.date.available2012-05-29T06:10:22Z-
dc.date.issued1994en_US
dc.identifier.citationAmerican Journal Of Clinical Pathology, 1994, v. 102 n. 1, p. 80-86en_US
dc.identifier.issn0002-9173en_US
dc.identifier.urihttp://hdl.handle.net/10722/148013-
dc.description.abstractTo examine the prognostic and pathobiologic significance of DNA content, the authors studied the surgically resected hepatocellular carcinomas of 69 patients by flow cytometric analysis. Homogeneity of DNA content within individual tumor nodules was present in 15 (88%) of 17 specimens examined. Similarly, homogeneity of DNA content in tumors having multiple nodules was found in 8 (73%) of 11 specimens. In 64 tumors with homogeneous DNA content evaluated further, DNA aneuploidy was present in 30 (46.9%) specimens, and the proportion of aneuploid tumors was similar in the large (> 5 cm in diameter, n = 35) and small (≤ 5 cm, n = 25) lesions, at 42.9% and 40%, respectively. Overall, the diploid tumors had serum α-fetoprotein levels increased to greater than 500 μg/mL more frequently than did the aneuploid tumors (P = .037). DNA content did not correlate significantly with hepatitis B surface antigen, presence of liver cirrhosis, cellular differentiation, tumor size, or tumor encapsulation. DNA content also did not influence tumor invasiveness in terms of liver invasion, presence of tumor microsatellites, or venous permeation. With multivariate Cox regression analysis, tumor encapsulation (P = .015), negative resection margin (P = .007), and DNA ploidy pattern stratified according to large and small tumors (P = .024) were favorable prognostic factors. In the small tumors, a diploid DNA pattern was associated with significantly better patient survival than was an aneuploid pattern (P = .012). In the large tumors, on the contrary, a diploid pattern was associated with poorer patient survival than was an aneuploid pattern (P = .029). The authors conclude that DNA ploidy pattern in hepatocellular carcinomas is homogeneous and stable. It supplements other predictors in prognostication when the lesions are stratified into small and large ones by tumor size. It is of particular importance as a predictor because it can be assessed preoperatively in needle-biopsy specimens.en_US
dc.languageengen_US
dc.publisherAmerican Society for Clinical Pathology. The Journal's web site is located at http://www.ajcp.comen_US
dc.relation.ispartofAmerican Journal of Clinical Pathologyen_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshCarcinoma, Hepatocellular - Genetics - Mortality - Pathologyen_US
dc.subject.meshDna, Neoplasm - Analysisen_US
dc.subject.meshFemaleen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshHumansen_US
dc.subject.meshLiver Neoplasms - Genetics - Mortality - Pathologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPloidiesen_US
dc.subject.meshSurvival Analysisen_US
dc.titleFlow cytometric analysis of DNA ploidy in hepatocellular carcinomaen_US
dc.typeArticleen_US
dc.identifier.emailNg, IOL:iolng@hkucc.hku.hken_US
dc.identifier.authorityNg, IOL=rp00335en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8037171-
dc.identifier.scopuseid_2-s2.0-0028137277en_US
dc.identifier.volume102en_US
dc.identifier.issue1en_US
dc.identifier.spage80en_US
dc.identifier.epage86en_US
dc.identifier.isiWOS:A1994NY41600014-
dc.publisher.placeUnited Statesen_US

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