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Article: Racial variation in the O-acetylation phenotype of human colonic mucosa

TitleRacial variation in the O-acetylation phenotype of human colonic mucosa
Authors
Issue Date1994
PublisherJohn Wiley & Sons. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/1130
Citation
Journal Of Pathology, 1994, v. 174 n. 3, p. 169-174 How to Cite?
AbstractO-acetylated and non-O-acetylated sialoglycoproteins can be distinguished by the mPAS (mild periodic acid-Schiff) histochemical technique. Individual adults show one of three different patterns of staining of large intestinal mucosa: uniformly mPAS-positive, uniformly mPAS-negative, or mPAS-negative with scattered mPAS-positive crypts. To test our hypothesis that these variations are the result of a single autosomal gene (oat) polymorphism, we have studied the frequency of the three patterns of staining in a total of 435 adult colon specimens from six geographically separate populations: British, South African blacks, Icelanders, Japanese, Hong Kong Chinese, and Bahrainis. The distribution of the three types of staining fell into two groups. In Japanese and Chinese, uniformly mPAS-positive cases were much more frequent than uniformly mPAS-negative cases; this distribution differed significantly (χ 2 P<0.001) from that in non-Sine-Japanese, where the uniformly mPAS-positive phenotype was much less frequently found than the uniformly mPAS-negative phenotype. In neither of the groups did the frequency of the three phenotypes differ significantly from that predicted for a single gene polymorphism by the Hardy-Weinberg law. The variation in staining patterns between populations is consistent with variation in frequency of a single polymorphic autosomal gene (oat) controlling O-acetylation of sialic acid, probably by an O-acetyl transferase enzyme. Loss of function mutation in the high acetylator gene (oat a) in a colonic crypt stem cell in heterozygous individuals would account for the scattered discordant crypts. Gene frequencies for a variety of enzymes differ between the Sine-Japanese and non-Sine-Japanese races. This newly described gene polymorphism may be related to differential susceptibility to organisms binding specifically to either O-acetylated or non-O-acetylated sialoglycoproteins, or to differential enteric colonization by bacterial flora that vary in their relative secretion of sialidases and sialate O-acetyl esterases.
Persistent Identifierhttp://hdl.handle.net/10722/148000
ISSN
2015 Impact Factor: 7.381
2015 SCImago Journal Rankings: 4.176
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorCampbell, Fen_US
dc.contributor.authorAppleton, MACen_US
dc.contributor.authorFuller, CEen_US
dc.contributor.authorGreeff, MPen_US
dc.contributor.authorHallgrimsson, Jen_US
dc.contributor.authorKatoh, Ren_US
dc.contributor.authorNg, OLIen_US
dc.contributor.authorSatir, Aen_US
dc.contributor.authorWilliams, GTen_US
dc.contributor.authorWilliams, EDen_US
dc.date.accessioned2012-05-29T06:10:18Z-
dc.date.available2012-05-29T06:10:18Z-
dc.date.issued1994en_US
dc.identifier.citationJournal Of Pathology, 1994, v. 174 n. 3, p. 169-174en_US
dc.identifier.issn0022-3417en_US
dc.identifier.urihttp://hdl.handle.net/10722/148000-
dc.description.abstractO-acetylated and non-O-acetylated sialoglycoproteins can be distinguished by the mPAS (mild periodic acid-Schiff) histochemical technique. Individual adults show one of three different patterns of staining of large intestinal mucosa: uniformly mPAS-positive, uniformly mPAS-negative, or mPAS-negative with scattered mPAS-positive crypts. To test our hypothesis that these variations are the result of a single autosomal gene (oat) polymorphism, we have studied the frequency of the three patterns of staining in a total of 435 adult colon specimens from six geographically separate populations: British, South African blacks, Icelanders, Japanese, Hong Kong Chinese, and Bahrainis. The distribution of the three types of staining fell into two groups. In Japanese and Chinese, uniformly mPAS-positive cases were much more frequent than uniformly mPAS-negative cases; this distribution differed significantly (χ 2 P<0.001) from that in non-Sine-Japanese, where the uniformly mPAS-positive phenotype was much less frequently found than the uniformly mPAS-negative phenotype. In neither of the groups did the frequency of the three phenotypes differ significantly from that predicted for a single gene polymorphism by the Hardy-Weinberg law. The variation in staining patterns between populations is consistent with variation in frequency of a single polymorphic autosomal gene (oat) controlling O-acetylation of sialic acid, probably by an O-acetyl transferase enzyme. Loss of function mutation in the high acetylator gene (oat a) in a colonic crypt stem cell in heterozygous individuals would account for the scattered discordant crypts. Gene frequencies for a variety of enzymes differ between the Sine-Japanese and non-Sine-Japanese races. This newly described gene polymorphism may be related to differential susceptibility to organisms binding specifically to either O-acetylated or non-O-acetylated sialoglycoproteins, or to differential enteric colonization by bacterial flora that vary in their relative secretion of sialidases and sialate O-acetyl esterases.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/1130en_US
dc.relation.ispartofJournal of Pathologyen_US
dc.subject.meshAcetylationen_US
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAged, 80 And Overen_US
dc.subject.meshColon - Metabolismen_US
dc.subject.meshContinental Population Groupsen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Frequencyen_US
dc.subject.meshHeterozygoteen_US
dc.subject.meshHumansen_US
dc.subject.meshIntestinal Mucosa - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPeriodic Acid-Schiff Reactionen_US
dc.subject.meshPhenotypeen_US
dc.subject.meshPolymorphism, Geneticen_US
dc.subject.meshSialoglycoproteins - Genetics - Metabolismen_US
dc.titleRacial variation in the O-acetylation phenotype of human colonic mucosaen_US
dc.typeArticleen_US
dc.identifier.emailNg, OLI:iolng@hkucc.hku.hken_US
dc.identifier.authorityNg, OLI=rp00335en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/path.1711740305en_US
dc.identifier.pmid7823249en_US
dc.identifier.scopuseid_2-s2.0-0027959506en_US
dc.identifier.volume174en_US
dc.identifier.issue3en_US
dc.identifier.spage169en_US
dc.identifier.epage174en_US
dc.identifier.isiWOS:A1994PU25400004-
dc.publisher.placeUnited Kingdomen_US

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