File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Effect of dexamethasone on mRNA levels for 5-aminolevulinate synthase in different rat tissues

TitleEffect of dexamethasone on mRNA levels for 5-aminolevulinate synthase in different rat tissues
Authors
Issue Date1992
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJB
Citation
European Journal Of Biochemistry, 1992, v. 203 n. 1-2, p. 59-63 How to Cite?
Abstract5-Aminolevulinate synthase mRNA levels from different tissues were quantitated by Northern blot hybridization analysis utilizing the rat liver 5-aminolevulinate synthase cDNA clone as probe. A 5-aminolevulinate synthase mRNA species of size 2.3 kb was seen in all the tissues examined. Densitometric scanning of the autoradiographs demonstrated that the adrenal gland contained the largest amount of 5-aminolevulinate synthase mRNA. Levels corresponding to approximately 50% of this amount were found in the small intestine, lung, heart, muscle and testes. In the liver and kidney the level was approximately 25% of that found in the adrenal gland. These results demonstrate the housekeeping role of this gene. Dexamethasone treatment for 1 day or 5 days dramatically induced 5-aminolevulinate synthase mRNA levels in the liver and small intestine, and to a lesser extent in lung, heart, kidney and muscle. Nuclear run-off experiments suggest that a post-transcriptional mechanism predominantly contributes to the dexamethasone-induced increase in 5-aminolevulinate synthase mRNA levels observed in the liver. Interestingly, in the steroidogenic tissues of the adrenal gland and testes, there was a substantial decrease in 5-aminolevulinate synthase mRNA levels after dexamethasone administration but the mechanism of this control remains to be investigated.
Persistent Identifierhttp://hdl.handle.net/10722/147910
ISSN
2006 Impact Factor: 3.579
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSrivastava, Gen_HK
dc.contributor.authorKwong, SKYen_HK
dc.contributor.authorLam, KSLen_HK
dc.contributor.authorMay, BKen_HK
dc.date.accessioned2012-05-29T06:09:51Z-
dc.date.available2012-05-29T06:09:51Z-
dc.date.issued1992en_HK
dc.identifier.citationEuropean Journal Of Biochemistry, 1992, v. 203 n. 1-2, p. 59-63en_HK
dc.identifier.issn0014-2956en_HK
dc.identifier.urihttp://hdl.handle.net/10722/147910-
dc.description.abstract5-Aminolevulinate synthase mRNA levels from different tissues were quantitated by Northern blot hybridization analysis utilizing the rat liver 5-aminolevulinate synthase cDNA clone as probe. A 5-aminolevulinate synthase mRNA species of size 2.3 kb was seen in all the tissues examined. Densitometric scanning of the autoradiographs demonstrated that the adrenal gland contained the largest amount of 5-aminolevulinate synthase mRNA. Levels corresponding to approximately 50% of this amount were found in the small intestine, lung, heart, muscle and testes. In the liver and kidney the level was approximately 25% of that found in the adrenal gland. These results demonstrate the housekeeping role of this gene. Dexamethasone treatment for 1 day or 5 days dramatically induced 5-aminolevulinate synthase mRNA levels in the liver and small intestine, and to a lesser extent in lung, heart, kidney and muscle. Nuclear run-off experiments suggest that a post-transcriptional mechanism predominantly contributes to the dexamethasone-induced increase in 5-aminolevulinate synthase mRNA levels observed in the liver. Interestingly, in the steroidogenic tissues of the adrenal gland and testes, there was a substantial decrease in 5-aminolevulinate synthase mRNA levels after dexamethasone administration but the mechanism of this control remains to be investigated.en_HK
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/EJBen_HK
dc.relation.ispartofEuropean Journal of Biochemistryen_HK
dc.subject.mesh5-Aminolevulinate Synthetase - Genetics - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAutoradiographyen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshDexamethasone - Pharmacologyen_US
dc.subject.meshMaleen_US
dc.subject.meshRna, Messenger - Drug Effects - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.subject.meshTissue Distributionen_US
dc.subject.meshTranscription, Geneticen_US
dc.titleEffect of dexamethasone on mRNA levels for 5-aminolevulinate synthase in different rat tissuesen_HK
dc.typeArticleen_HK
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_HK
dc.identifier.emailLam, KSL:ksllam@hku.hken_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.identifier.authorityLam, KSL=rp00343en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1730241-
dc.identifier.scopuseid_2-s2.0-0026531621en_HK
dc.identifier.volume203en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage59en_HK
dc.identifier.epage63en_HK
dc.identifier.isiWOS:A1992HA78000006-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK
dc.identifier.scopusauthoridKwong, SKY=13304969800en_HK
dc.identifier.scopusauthoridLam, KSL=8082870600en_HK
dc.identifier.scopusauthoridMay, BK=7202910361en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats