File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Estrogen regulates the gene expression of vasoactive intestinal peptide in the anterior pituitary

TitleEstrogen regulates the gene expression of vasoactive intestinal peptide in the anterior pituitary
Authors
Keywordsanterior pituitary
estrogen
gene expression
vasoactive intestinal peptide
Issue Date1990
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/NEN
Citation
Neuroendocrinology, 1990, v. 52 n. 5, p. 417-421 How to Cite?
AbstractVasoactive intestinal peptide (VIP), a prolactin (PRL)-releasing factor, has been shown to be synthesized within the anterior pituitary. To test the hypothesis that estrogens increase PRL secretion, at least in part, by stimulating VIP secretion, the concentrations of VIP, peptide histidine isoleucine (PHI) and prepro VIP mRNA were measured in the anterior pituitaries of oophorectomized rats treated with 17β-estradiol benzoate 25 μg/kg/day s.c. for 5 days. For comparison, changes in the hypothalamus were also measured. Estrogen treatment resulted in a marked increase in pituitary VIP content without detectable changes in PHI content, suggesting that estrogen may regulate differentially the enzymes involved in the posttranslational processing of the VIP prohormone. A VIP mRNA-transcript of about 1.7 kilobases was detected in all tissues studied, being most abundant in the cortex, less abundant in the hypothalmus and barely detectable in the untreated pituitary. Estrogen treatment resulted in an increase in VIP gene expression in the pituitary but not in the hypothalamus or cerebral cortex. This marked increase in prepro VIP mRNA rendered possible the demonstration in the estrogen-treated pituitary of a second VIP transcript of about 1.0 kilobase which was present in only very low quantities in the cortex and hypothalamus. We conclude that estrogen regulates the gene expression of VIP in the anterior pituitary. Changes in VIP secretion may contribute to the stimulatory effect of estrogen on PRL secretion.
Persistent Identifierhttp://hdl.handle.net/10722/147851
ISSN
2015 Impact Factor: 2.583
2015 SCImago Journal Rankings: 1.479
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLam, KSLen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.contributor.authorLechan, RMen_HK
dc.contributor.authorLee, Ten_HK
dc.contributor.authorReichlin, Sen_HK
dc.date.accessioned2012-05-29T06:09:33Z-
dc.date.available2012-05-29T06:09:33Z-
dc.date.issued1990en_HK
dc.identifier.citationNeuroendocrinology, 1990, v. 52 n. 5, p. 417-421en_HK
dc.identifier.issn0028-3835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/147851-
dc.description.abstractVasoactive intestinal peptide (VIP), a prolactin (PRL)-releasing factor, has been shown to be synthesized within the anterior pituitary. To test the hypothesis that estrogens increase PRL secretion, at least in part, by stimulating VIP secretion, the concentrations of VIP, peptide histidine isoleucine (PHI) and prepro VIP mRNA were measured in the anterior pituitaries of oophorectomized rats treated with 17β-estradiol benzoate 25 μg/kg/day s.c. for 5 days. For comparison, changes in the hypothalamus were also measured. Estrogen treatment resulted in a marked increase in pituitary VIP content without detectable changes in PHI content, suggesting that estrogen may regulate differentially the enzymes involved in the posttranslational processing of the VIP prohormone. A VIP mRNA-transcript of about 1.7 kilobases was detected in all tissues studied, being most abundant in the cortex, less abundant in the hypothalmus and barely detectable in the untreated pituitary. Estrogen treatment resulted in an increase in VIP gene expression in the pituitary but not in the hypothalamus or cerebral cortex. This marked increase in prepro VIP mRNA rendered possible the demonstration in the estrogen-treated pituitary of a second VIP transcript of about 1.0 kilobase which was present in only very low quantities in the cortex and hypothalamus. We conclude that estrogen regulates the gene expression of VIP in the anterior pituitary. Changes in VIP secretion may contribute to the stimulatory effect of estrogen on PRL secretion.en_HK
dc.languageengen_US
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/NENen_HK
dc.relation.ispartofNeuroendocrinologyen_HK
dc.subjectanterior pituitaryen_HK
dc.subjectestrogenen_HK
dc.subjectgene expressionen_HK
dc.subjectvasoactive intestinal peptideen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshCerebral Cortex - Metabolismen_US
dc.subject.meshChromatography, Gelen_US
dc.subject.meshEstrogens - Blood - Physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Regulation - Physiologyen_US
dc.subject.meshHypothalamus - Metabolismen_US
dc.subject.meshOvary - Physiologyen_US
dc.subject.meshPeptide Phi - Metabolismen_US
dc.subject.meshPituitary Gland, Anterior - Metabolismen_US
dc.subject.meshProlactin - Blooden_US
dc.subject.meshProtein Precursors - Geneticsen_US
dc.subject.meshRna, Messenger - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.subject.meshVasoactive Intestinal Peptide - Geneticsen_US
dc.titleEstrogen regulates the gene expression of vasoactive intestinal peptide in the anterior pituitaryen_HK
dc.typeArticleen_HK
dc.identifier.emailLam, KSL:ksllam@hku.hken_HK
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_HK
dc.identifier.authorityLam, KSL=rp00343en_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid2126350-
dc.identifier.scopuseid_2-s2.0-0025013054en_HK
dc.identifier.volume52en_HK
dc.identifier.issue5en_HK
dc.identifier.spage417en_HK
dc.identifier.epage421en_HK
dc.identifier.isiWOS:A1990EH35800001-
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridLam, KSL=8082870600en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK
dc.identifier.scopusauthoridLechan, RM=7005636129en_HK
dc.identifier.scopusauthoridLee, T=7501439535en_HK
dc.identifier.scopusauthoridReichlin, S=16186732300en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats