File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: PU.1 can recruit BCL6 to DNA to repress gene expression in germinal center B cells

TitlePU.1 can recruit BCL6 to DNA to repress gene expression in germinal center B cells
Authors
Issue Date2009
Citation
Molecular And Cellular Biology, 2009, v. 29 n. 17, p. 4612-4622 How to Cite?
AbstractBCL6 is a transcriptional repressor crucial for germinal center formation. BCL6 represses transcription by a variety of mechanisms by binding to specific DNA sequences or by recruitment to DNA by protein interactions. We found that BCL6 can inhibit activities of the immunoglobulin kappa (Igκ) intron and 3′ enhancers. At the Igκ 3′ enhancer, BCL6 repressed enhancer activity through the PU.1 binding site. We found that BCL6 physically interacted with PU.1 in vivo and in vitro, and the results of sequential chromatin immunoprecipitation assays and transient-expression assays suggested that BCL6 recruitment to the Igκ and Igλ 3′ enhancers occurred via PU.1 interaction. By computational studies, we identified genes that are repressed in germinal center cells and whose promoters contain conserved PU.1 binding sites in mouse and human. We found that many of these promoters bound to both PU.1 and BCL6 in vivo. In addition, BCL6 knockdown resulted in increased expression of a subset of these genes, demonstrating that BCL6 is involved in their repression. The recruitment of BCL6 to promoter regions by PU.1 represents a new regulatory mechanism that expands the number of genes regulated by this important transcriptional repressor. Copyright © 2009, American Society for Microbiology. All Rights Reserved.
Persistent Identifierhttp://hdl.handle.net/10722/147607
ISSN
2015 Impact Factor: 4.427
2015 SCImago Journal Rankings: 3.806
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
NIHGM071830
University Grants Committee of Hong KongA.E./M-04/04
Funding Information:

This work was supported by NIH grant GM071830 (M.L.A.) and by grant A.E./M-04/04 from the University Grants Committee of Hong Kong (J.W.).

References

 

DC FieldValueLanguage
dc.contributor.authorWei, Fen_US
dc.contributor.authorZaprazna, Ken_US
dc.contributor.authorWang, Jen_US
dc.contributor.authorAtchison, MLen_US
dc.date.accessioned2012-05-29T06:04:56Z-
dc.date.available2012-05-29T06:04:56Z-
dc.date.issued2009en_US
dc.identifier.citationMolecular And Cellular Biology, 2009, v. 29 n. 17, p. 4612-4622en_US
dc.identifier.issn0270-7306en_US
dc.identifier.urihttp://hdl.handle.net/10722/147607-
dc.description.abstractBCL6 is a transcriptional repressor crucial for germinal center formation. BCL6 represses transcription by a variety of mechanisms by binding to specific DNA sequences or by recruitment to DNA by protein interactions. We found that BCL6 can inhibit activities of the immunoglobulin kappa (Igκ) intron and 3′ enhancers. At the Igκ 3′ enhancer, BCL6 repressed enhancer activity through the PU.1 binding site. We found that BCL6 physically interacted with PU.1 in vivo and in vitro, and the results of sequential chromatin immunoprecipitation assays and transient-expression assays suggested that BCL6 recruitment to the Igκ and Igλ 3′ enhancers occurred via PU.1 interaction. By computational studies, we identified genes that are repressed in germinal center cells and whose promoters contain conserved PU.1 binding sites in mouse and human. We found that many of these promoters bound to both PU.1 and BCL6 in vivo. In addition, BCL6 knockdown resulted in increased expression of a subset of these genes, demonstrating that BCL6 is involved in their repression. The recruitment of BCL6 to promoter regions by PU.1 represents a new regulatory mechanism that expands the number of genes regulated by this important transcriptional repressor. Copyright © 2009, American Society for Microbiology. All Rights Reserved.en_US
dc.languageengen_US
dc.relation.ispartofMolecular and Cellular Biologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshB-Lymphocytes - Cytology - Physiologyen_US
dc.subject.meshDna - Genetics - Metabolismen_US
dc.subject.meshEnhancer Elements, Geneticen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshGerminal Center - Cytologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin Lambda-Chains - Geneticsen_US
dc.subject.meshImmunoglobulins - Geneticsen_US
dc.subject.meshIntronsen_US
dc.subject.meshMiceen_US
dc.subject.meshPromoter Regions, Geneticen_US
dc.subject.meshProto-Oncogene Proteins - Genetics - Metabolismen_US
dc.subject.meshProto-Oncogene Proteins C-Bcl-6 - Genetics - Metabolismen_US
dc.subject.meshTrans-Activators - Genetics - Metabolismen_US
dc.titlePU.1 can recruit BCL6 to DNA to repress gene expression in germinal center B cellsen_US
dc.typeArticleen_US
dc.identifier.emailWang, J:junwen@hkucc.hku.hken_US
dc.identifier.authorityWang, J=rp00280en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1128/MCB.00234-09en_US
dc.identifier.pmid19564417-
dc.identifier.pmcidPMC2725722-
dc.identifier.scopuseid_2-s2.0-68849111429en_US
dc.identifier.hkuros158846-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-68849111429&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume29en_US
dc.identifier.issue17en_US
dc.identifier.spage4612en_US
dc.identifier.epage4622en_US
dc.identifier.isiWOS:000268813100003-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWei, F=55186434600en_US
dc.identifier.scopusauthoridZaprazna, K=32868288900en_US
dc.identifier.scopusauthoridWang, J=8950599500en_US
dc.identifier.scopusauthoridAtchison, ML=7006495633en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats