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Article: HTLV-I Tax self-association in optimal trans-activation function

TitleHTLV-I Tax self-association in optimal trans-activation function
Authors
Issue Date1997
PublisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/
Citation
Nucleic Acids Research, 1997, v. 25 n. 2, p. 379-387 How to Cite?
AbstractHTLV-I Tax protein is a potent transcriptional activator of viral and cellular genes. Tax does not bind DNA directly but interacts through protein-protein contact with host cell factors that recognize the viral long terminal repeat (LTR). Domains within Tax needed for protein-protein interaction have not been fully characterized. In studying transcriptional function in yeast cells, we unexpectedly found that Tax functions optimally not as a monomer, but as a homodimer. Here we have used the one hybrid and two hybrid genetic approaches in yeast to investigate the region(s) within Tax necessary for self-association. Dimer formation was also confirmed biochemically by using electrophoretic mobility shift (EMSA) and supershift assays. Twenty two Tax point mutants were utilized to map relevant residues. Genetic results from this series of mutants revealed that a necessary region for dimerization is contained within a previously characterized zinc finger domain. Two loss-of-function Tax mutants, each poorly active when assayed individually, were found to have complementing activity when co-expressed together. This genetic complementation suggests a mechanism for trans-activation resulting from simultaneous but non-identical contact with a responsive target by each of two Tax monomers in a dimer.
Persistent Identifierhttp://hdl.handle.net/10722/147414
ISSN
2015 Impact Factor: 9.202
2015 SCImago Journal Rankings: 7.458
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJin, DYen_US
dc.contributor.authorJeang, KTen_US
dc.date.accessioned2012-05-29T06:03:33Z-
dc.date.available2012-05-29T06:03:33Z-
dc.date.issued1997en_US
dc.identifier.citationNucleic Acids Research, 1997, v. 25 n. 2, p. 379-387en_US
dc.identifier.issn0305-1048en_US
dc.identifier.urihttp://hdl.handle.net/10722/147414-
dc.description.abstractHTLV-I Tax protein is a potent transcriptional activator of viral and cellular genes. Tax does not bind DNA directly but interacts through protein-protein contact with host cell factors that recognize the viral long terminal repeat (LTR). Domains within Tax needed for protein-protein interaction have not been fully characterized. In studying transcriptional function in yeast cells, we unexpectedly found that Tax functions optimally not as a monomer, but as a homodimer. Here we have used the one hybrid and two hybrid genetic approaches in yeast to investigate the region(s) within Tax necessary for self-association. Dimer formation was also confirmed biochemically by using electrophoretic mobility shift (EMSA) and supershift assays. Twenty two Tax point mutants were utilized to map relevant residues. Genetic results from this series of mutants revealed that a necessary region for dimerization is contained within a previously characterized zinc finger domain. Two loss-of-function Tax mutants, each poorly active when assayed individually, were found to have complementing activity when co-expressed together. This genetic complementation suggests a mechanism for trans-activation resulting from simultaneous but non-identical contact with a responsive target by each of two Tax monomers in a dimer.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://nar.oxfordjournals.org/en_US
dc.relation.ispartofNucleic Acids Researchen_US
dc.subject.meshChloramphenicol O-Acetyltransferase - Genetics - Metabolismen_US
dc.subject.meshCloning, Molecularen_US
dc.subject.meshDimerizationen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshGene Expression - Geneticsen_US
dc.subject.meshGene Products, Tax - Chemistry - Metabolismen_US
dc.subject.meshGenetic Complementation Testen_US
dc.subject.meshModels, Chemicalen_US
dc.subject.meshMutagenesis, Site-Directed - Geneticsen_US
dc.subject.meshPoint Mutation - Geneticsen_US
dc.subject.meshSaccharomyces Cerevisiae - Geneticsen_US
dc.subject.meshSequence Deletion - Geneticsen_US
dc.subject.meshTranscriptional Activation - Geneticsen_US
dc.subject.meshViral Proteins - Chemistry - Metabolismen_US
dc.subject.meshZinc Fingers - Geneticsen_US
dc.subject.meshBeta-Galactosidase - Genetics - Metabolismen_US
dc.titleHTLV-I Tax self-association in optimal trans-activation functionen_US
dc.typeArticleen_US
dc.identifier.emailJin, DY:dyjin@hkucc.hku.hken_US
dc.identifier.authorityJin, DY=rp00452en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/nar/25.2.379en_US
dc.identifier.pmid9016568-
dc.identifier.scopuseid_2-s2.0-0030831166en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030831166&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume25en_US
dc.identifier.issue2en_US
dc.identifier.spage379en_US
dc.identifier.epage387en_US
dc.identifier.isiWOS:A1997WD44400014-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridJin, DY=7201973614en_US
dc.identifier.scopusauthoridJeang, KT=7004824803en_US

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