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Article: Synthesis and expression in Escherichia coli of a human neutrophil activating protein-1/interleukin-8 gene.

TitleSynthesis and expression in Escherichia coli of a human neutrophil activating protein-1/interleukin-8 gene.
Authors
Issue Date1993
PublisherScience in China Press. The Journal's website is located at http://chem.scichina.com:8081/sciBe/EN/volumn/current.shtml
Citation
Science In China. Series B, Chemistry, Life Sciences & Earth Sciences, 1993, v. 36 n. 10, p. 1224-1232 How to Cite?
AbstractThe complete gene coding for human neutrophil activating protein-1/interleukin-8 was synthesized using a semi-chemical semi-enzymatic method. The synthetic gene was then overexpressed in Escherichia coli under the temperature-regulated control of the PRPL tandem promoters. As determined by SDS-PAGE and densitometry, the overexpressed protein comprised up to 18.5% and 10.9% of the total soluble protein in E. coli cells grown in shake flasks and in batch fermentation, respectively. The recombinant NAP-1/IL-8 was then purified to > 95% homogeneity by gel filtration and cation exchange chromatography. The purified protein appeared as a single band on the SDS-PAGE gel and possessed potent chemotactic activity in the concentration of < 10 ng/ml, as assayed by the agarose plate method. An early skin reactivity was also observed when the pure NAP-1/IL-8 was injected subcutaneously into the rabbits. The N-terminal 36 amino acid sequence of the recombinant NAP-1/IL-8 was determined using the Edman method and was shown to be identical to that of the native protein.
Persistent Identifierhttp://hdl.handle.net/10722/147386
ISSN
1998 Impact Factor: 0.479
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorJin, DYen_US
dc.contributor.authorXu, RHen_US
dc.contributor.authorZhou, Yen_US
dc.contributor.authorWang, Pen_US
dc.contributor.authorHou, YDen_US
dc.date.accessioned2012-05-29T06:03:20Z-
dc.date.available2012-05-29T06:03:20Z-
dc.date.issued1993en_US
dc.identifier.citationScience In China. Series B, Chemistry, Life Sciences & Earth Sciences, 1993, v. 36 n. 10, p. 1224-1232en_US
dc.identifier.issn1001-652Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/147386-
dc.description.abstractThe complete gene coding for human neutrophil activating protein-1/interleukin-8 was synthesized using a semi-chemical semi-enzymatic method. The synthetic gene was then overexpressed in Escherichia coli under the temperature-regulated control of the PRPL tandem promoters. As determined by SDS-PAGE and densitometry, the overexpressed protein comprised up to 18.5% and 10.9% of the total soluble protein in E. coli cells grown in shake flasks and in batch fermentation, respectively. The recombinant NAP-1/IL-8 was then purified to > 95% homogeneity by gel filtration and cation exchange chromatography. The purified protein appeared as a single band on the SDS-PAGE gel and possessed potent chemotactic activity in the concentration of < 10 ng/ml, as assayed by the agarose plate method. An early skin reactivity was also observed when the pure NAP-1/IL-8 was injected subcutaneously into the rabbits. The N-terminal 36 amino acid sequence of the recombinant NAP-1/IL-8 was determined using the Edman method and was shown to be identical to that of the native protein.en_US
dc.languageengen_US
dc.publisherScience in China Press. The Journal's website is located at http://chem.scichina.com:8081/sciBe/EN/volumn/current.shtml-
dc.relation.ispartofScience in China. Series B, Chemistry, life sciences & earth sciencesen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBiological Assayen_US
dc.subject.meshDna - Geneticsen_US
dc.subject.meshEscherichia Coli - Genetics - Metabolismen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshGenes, Syntheticen_US
dc.subject.meshHumansen_US
dc.subject.meshInterleukin-8 - Biosynthesis - Geneticsen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshRabbitsen_US
dc.titleSynthesis and expression in Escherichia coli of a human neutrophil activating protein-1/interleukin-8 gene.en_US
dc.typeArticleen_US
dc.identifier.emailJin, DY:dyjin@hkucc.hku.hken_US
dc.identifier.authorityJin, DY=rp00452en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid8136035-
dc.identifier.scopuseid_2-s2.0-0027683552en_US
dc.identifier.scopuseid_2-s2.0-43949165981-
dc.identifier.volume36en_US
dc.identifier.issue10en_US
dc.identifier.spage1224en_US
dc.identifier.epage1232en_US
dc.identifier.isiWOS:A1993MJ33200009-
dc.publisher.placeChina-
dc.identifier.scopusauthoridJin, DY=7201973614en_US
dc.identifier.scopusauthoridXu, RH=7402814245en_US
dc.identifier.scopusauthoridZhou, Y=7405368674en_US
dc.identifier.scopusauthoridWang, P=7405457756en_US
dc.identifier.scopusauthoridHou, YD=7402198565en_US

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