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Article: Evaluation of the purity of recombinant proteins and detection of residual protein contaminants via N-terminal microsequencing and database searching.

TitleEvaluation of the purity of recombinant proteins and detection of residual protein contaminants via N-terminal microsequencing and database searching.
Authors
Issue Date1991
Citation
Chinese Journal Of Biotechnology, 1991, v. 7 n. 2, p. 105-111 How to Cite?
AbstractThe N-terminal amino acid sequences of purified recombinant human gamma-interferon, alpha 2a-interferon and interleukin-2 expressed in E. coli were determined on an Applied Biosystems 477A Protein/Peptide Sequencer and 120A PTH Amino Acid Analyzer. From the raw chromatographic data of these samples, the identity, heterogeneity, amount of methionine-plus species remaining in the final products, and the probable process contaminants were evaluated with the help of computer methods including database searching. General methods to characterize trace contaminants in protein samples were also discussed. Among the sequenced samples, only gamma-interferon was shown to be N-terminal homogeneous. Methionine-containing species were found in interleukin-2 and alpha 2a-interferon. Chicken eggwhite lysozyme was detected in very small amounts in one batch of samples. These results provide valuable information for the development and improvement of preparation methods as well as regulatory responses to recombinant products.
Persistent Identifierhttp://hdl.handle.net/10722/147364
ISSN
2011 SCImago Journal Rankings: 0.270

 

DC FieldValueLanguage
dc.contributor.authorJin, DYen_US
dc.contributor.authorZhang, ZQen_US
dc.contributor.authorZhou, YAen_US
dc.contributor.authorHou, YDen_US
dc.date.accessioned2012-05-29T06:03:12Z-
dc.date.available2012-05-29T06:03:12Z-
dc.date.issued1991en_US
dc.identifier.citationChinese Journal Of Biotechnology, 1991, v. 7 n. 2, p. 105-111en_US
dc.identifier.issn1042-749Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/147364-
dc.description.abstractThe N-terminal amino acid sequences of purified recombinant human gamma-interferon, alpha 2a-interferon and interleukin-2 expressed in E. coli were determined on an Applied Biosystems 477A Protein/Peptide Sequencer and 120A PTH Amino Acid Analyzer. From the raw chromatographic data of these samples, the identity, heterogeneity, amount of methionine-plus species remaining in the final products, and the probable process contaminants were evaluated with the help of computer methods including database searching. General methods to characterize trace contaminants in protein samples were also discussed. Among the sequenced samples, only gamma-interferon was shown to be N-terminal homogeneous. Methionine-containing species were found in interleukin-2 and alpha 2a-interferon. Chicken eggwhite lysozyme was detected in very small amounts in one batch of samples. These results provide valuable information for the development and improvement of preparation methods as well as regulatory responses to recombinant products.en_US
dc.languageengen_US
dc.relation.ispartofChinese journal of biotechnologyen_US
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshCloning, Molecular - Methodsen_US
dc.subject.meshCystine - Metabolismen_US
dc.subject.meshDatabases, Factualen_US
dc.subject.meshEscherichia Coli - Genetics - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshInterferon-Alpha - Chemistry - Genetics - Isolation & Purificationen_US
dc.subject.meshInterferon-Gamma - Chemistry - Genetics - Isolation & Purificationen_US
dc.subject.meshInterleukin-2 - Chemistry - Genetics - Isolation & Purificationen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshOxidation-Reductionen_US
dc.subject.meshRecombinant Proteins - Chemistry - Isolation & Purificationen_US
dc.titleEvaluation of the purity of recombinant proteins and detection of residual protein contaminants via N-terminal microsequencing and database searching.en_US
dc.typeArticleen_US
dc.identifier.emailJin, DY:dyjin@hkucc.hku.hken_US
dc.identifier.authorityJin, DY=rp00452en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid1806020-
dc.identifier.scopuseid_2-s2.0-0026292110en_US
dc.identifier.volume7en_US
dc.identifier.issue2en_US
dc.identifier.spage105en_US
dc.identifier.epage111en_US
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridJin, DY=7201973614en_US
dc.identifier.scopusauthoridZhang, ZQ=8606786700en_US
dc.identifier.scopusauthoridZhou, YA=7405368674en_US
dc.identifier.scopusauthoridHou, YD=7402198565en_US

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