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Article: Molecular characterization of suppression of hepatitis B virus transcription by hepatitis C virus core protein

TitleMolecular characterization of suppression of hepatitis B virus transcription by hepatitis C virus core protein
Authors
KeywordsCore Protein
Gene Regulation
Hepatitis B Virus (Hbv)
Hepatitis C Virus (Hcv)
Issue Date1997
Publisher中國科學院. The Journal's web site is located at http://jcxg.chinajournal.net.cn/
Citation
Science In China, Series C: Life Sciences, 1997, v. 40 n. 6, p. 648-656 How to Cite?
AbstractTo further elucidate the molecular mechanisms underlying the suppression of hepatitis B virus (HBV) expression by the hepatitis C virus (HCV) core protein, five molecular clones of HCV cDNA sequence containing the 5′ noncoding (5′NC) and the core regions have been isolated from Chinese HBV- and HCV-coinfected patients. Sequence comparison and phylogenetic analysis showed that the HCV sequence cloned from coinfected individuals is indistinguishable from that identified in other patients. Cotransfection assay confirmed that the core protein expressed from one of the cloned sequence is capable of suppressing the expression of hepatitis B surface and e antigens (HBsAg and HBeAg, respectively). Deletion mapping revealed that the C-terminal hydrophobic region of the HCV core is necessary for the suppression. Results from reporter assays demonstrated that HCV core protein interacts with the HBV C promoter and enhancer II elements and down-regulates the transcription of HBV as well as other cellular and heterologous viral genes in both hepatic and non-hepatic cell lines. Taken together, the findings suggest HCV core protein as a multifunctional negative regulator of transcription critically involved in the molecular interactions between HBV and HCV, and between HCV and the cell.
Persistent Identifierhttp://hdl.handle.net/10722/147293
ISSN
2011 Impact Factor: 1.61
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Hen_US
dc.contributor.authorYan, Zen_US
dc.contributor.authorHou, Yen_US
dc.contributor.authorJin, Den_US
dc.date.accessioned2012-05-29T06:02:44Z-
dc.date.available2012-05-29T06:02:44Z-
dc.date.issued1997en_US
dc.identifier.citationScience In China, Series C: Life Sciences, 1997, v. 40 n. 6, p. 648-656en_US
dc.identifier.issn1006-9305en_US
dc.identifier.urihttp://hdl.handle.net/10722/147293-
dc.description.abstractTo further elucidate the molecular mechanisms underlying the suppression of hepatitis B virus (HBV) expression by the hepatitis C virus (HCV) core protein, five molecular clones of HCV cDNA sequence containing the 5′ noncoding (5′NC) and the core regions have been isolated from Chinese HBV- and HCV-coinfected patients. Sequence comparison and phylogenetic analysis showed that the HCV sequence cloned from coinfected individuals is indistinguishable from that identified in other patients. Cotransfection assay confirmed that the core protein expressed from one of the cloned sequence is capable of suppressing the expression of hepatitis B surface and e antigens (HBsAg and HBeAg, respectively). Deletion mapping revealed that the C-terminal hydrophobic region of the HCV core is necessary for the suppression. Results from reporter assays demonstrated that HCV core protein interacts with the HBV C promoter and enhancer II elements and down-regulates the transcription of HBV as well as other cellular and heterologous viral genes in both hepatic and non-hepatic cell lines. Taken together, the findings suggest HCV core protein as a multifunctional negative regulator of transcription critically involved in the molecular interactions between HBV and HCV, and between HCV and the cell.en_US
dc.languageengen_US
dc.publisher中國科學院. The Journal's web site is located at http://jcxg.chinajournal.net.cn/zh_HK
dc.relation.ispartofScience in China, Series C: Life Sciencesen_US
dc.subjectCore Proteinen_US
dc.subjectGene Regulationen_US
dc.subjectHepatitis B Virus (Hbv)en_US
dc.subjectHepatitis C Virus (Hcv)en_US
dc.titleMolecular characterization of suppression of hepatitis B virus transcription by hepatitis C virus core proteinen_US
dc.typeArticleen_US
dc.identifier.emailJin, D:dyjin@hkucc.hku.hken_US
dc.identifier.authorityJin, D=rp00452en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-0007401307en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0007401307&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume40en_US
dc.identifier.issue6en_US
dc.identifier.spage648en_US
dc.identifier.epage656en_US
dc.publisher.placeChinaen_US
dc.identifier.scopusauthoridWang, H=8081373900en_US
dc.identifier.scopusauthoridYan, Z=7402519934en_US
dc.identifier.scopusauthoridHou, Y=7402198565en_US
dc.identifier.scopusauthoridJin, D=7201973614en_US

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