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Conference Paper: C-terminus-truncated hepatitis B virus X protein enhances cell invasiveness in hepatocellular carcinoma

TitleC-terminus-truncated hepatitis B virus X protein enhances cell invasiveness in hepatocellular carcinoma
Authors
Issue Date2012
PublisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/
Citation
The 103rd Annual Meeting of the American Association for Cancer Research (AACR 2012), Chicago, IL., 31 March-4 April 2012. In Cancer Research, 2012, v. 72 n. 8 suppl., abstract no. 2212 How to Cite?
AbstractHepatocellular carcinoma (HCC) is the fifth of major malignancies worldwide and chronic hepatitis B virus (HBV) infection is one of its well known risk factors. Random integration of the HBV DNA into host genome is frequent in human HCCs and this leads to truncation of the HBV DNA, particularly at the C-terminal end of the HBV X protein (HBx). In this study, we investigated the frequency of this C-terminal truncation of HBx in human HCCs and its functional significance in HCC cells. In the 51 HBV-positive patients with HCC, full-length HBx was detected in all of the non-tumorous livers. Interestingly, natural COOH-truncated HBx was found in 47% tumours. Upon clinicopathological analysis, presence of natural COOH-truncated HBx significantly correlated with the presence of venous invasion, a hallmark of metastasis (p < 0.01). Doxycycline- inducible stable expression of the full-length HBx and C-terminal truncated forms of HBx in HepG2 cells was employed for in vitro cell invasion assay and cell signalling analysis involved in cell invasiveness. Inducible expression of COOH-truncated HBx protein (with 24 amino acids truncated at C-terminal end) in HepG2 cells enhanced cell invasiveness, and C-Jun transcriptional activity, and increased MMP10 transcription production, as compared with the full-length HBx. The activation of the MMP10 promoter by the COOH-truncated HBx was abolished when the AP-1 binding sites were mutated. Our data suggest that COOH-truncation of HBx may play a significant role in enhancing cell invasiveness and metastasis in HCC, possibly via activation of the MMP10 through C-Jun signaling pathway.
DescriptionPoster Session 8 - Oncogenic Gene Regulatory Mechanisms: abstract no. 2212
Open Access Journal
Persistent Identifierhttp://hdl.handle.net/10722/146448
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372

 

DC FieldValueLanguage
dc.contributor.authorSze, KMF-
dc.contributor.authorChu, GKY-
dc.contributor.authorNg, IOL-
dc.date.accessioned2012-04-24T07:54:40Z-
dc.date.available2012-04-24T07:54:40Z-
dc.date.issued2012-
dc.identifier.citationThe 103rd Annual Meeting of the American Association for Cancer Research (AACR 2012), Chicago, IL., 31 March-4 April 2012. In Cancer Research, 2012, v. 72 n. 8 suppl., abstract no. 2212-
dc.identifier.issn0008-5472-
dc.identifier.urihttp://hdl.handle.net/10722/146448-
dc.descriptionPoster Session 8 - Oncogenic Gene Regulatory Mechanisms: abstract no. 2212-
dc.descriptionOpen Access Journal-
dc.description.abstractHepatocellular carcinoma (HCC) is the fifth of major malignancies worldwide and chronic hepatitis B virus (HBV) infection is one of its well known risk factors. Random integration of the HBV DNA into host genome is frequent in human HCCs and this leads to truncation of the HBV DNA, particularly at the C-terminal end of the HBV X protein (HBx). In this study, we investigated the frequency of this C-terminal truncation of HBx in human HCCs and its functional significance in HCC cells. In the 51 HBV-positive patients with HCC, full-length HBx was detected in all of the non-tumorous livers. Interestingly, natural COOH-truncated HBx was found in 47% tumours. Upon clinicopathological analysis, presence of natural COOH-truncated HBx significantly correlated with the presence of venous invasion, a hallmark of metastasis (p < 0.01). Doxycycline- inducible stable expression of the full-length HBx and C-terminal truncated forms of HBx in HepG2 cells was employed for in vitro cell invasion assay and cell signalling analysis involved in cell invasiveness. Inducible expression of COOH-truncated HBx protein (with 24 amino acids truncated at C-terminal end) in HepG2 cells enhanced cell invasiveness, and C-Jun transcriptional activity, and increased MMP10 transcription production, as compared with the full-length HBx. The activation of the MMP10 promoter by the COOH-truncated HBx was abolished when the AP-1 binding sites were mutated. Our data suggest that COOH-truncation of HBx may play a significant role in enhancing cell invasiveness and metastasis in HCC, possibly via activation of the MMP10 through C-Jun signaling pathway.-
dc.languageeng-
dc.publisherAmerican Association for Cancer Research. The Journal's web site is located at http://cancerres.aacrjournals.org/-
dc.relation.ispartofCancer Research-
dc.titleC-terminus-truncated hepatitis B virus X protein enhances cell invasiveness in hepatocellular carcinoma-
dc.typeConference_Paper-
dc.identifier.emailSze, KMF: karensze@hkucc.hku.hk-
dc.identifier.emailChu, GKY: glanice@hku.hk-
dc.identifier.emailNg, IOL: iolng@hku.hk-
dc.identifier.authorityNg, IOL=rp00335-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1158/1538-7445.AM2012-2212-
dc.identifier.hkuros199227-
dc.identifier.volume72-
dc.identifier.issue8 suppl., abstract no. 2212-
dc.publisher.placeUnited States-
dc.description.otherThe 103rd Annual Meeting of the American Association for Cancer Research (AACR 2012), Chicago, IL., 31 March-4 April 2012.-

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