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Article: Measurement of liver T 1 and T 2 relaxation times in an experimental mouse model of liver fibrosis

TitleMeasurement of liver T 1 and T 2 relaxation times in an experimental mouse model of liver fibrosis
Authors
KeywordsCCl 4
liver fibrosis
MRI
T 1, T 2 relaxometry
Issue Date2012
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/1053-1807/
Citation
Journal Of Magnetic Resonance Imaging, 2012, v. 36 n. 1, p. 152-158 How to Cite?
Abstract
Purpose: To characterize changes in relaxation times of liver using quantitative magnetic resonance imaging (MRI) in an experimental mouse model of liver fibrosis. Quantitative MRI is a potentially robust method to characterize liver fibrosis. However, correlation between relaxation times and fibrosis stage has been controversial. Materials and Methods: Liver fibrosis was induced in male adult C57BL/6N mice (22-25 g; n = 12) by repetitive dosing of carbon tetrachloride (CCl 4). The animals were examined with a series of spin-echo (SE) images with varying TRs and multiecho SE imaging sequence at 7 T before and 2, 4, 6, and 8 weeks after CCl 4 insult. Hepatic T 1 and T 2 values were measured. Histology was performed with hematoxylin-eosin staining and Masson's trichrome staining. Results: Significant increase (P < 0.001) in hepatic T 1 was found at 2, 4, 6, and 8 weeks following CCl 4 insult as compared with that before insult. Meanwhile, hepatic T 2 at 2, 4, 6, and 8 weeks after CCl 4 insult was significantly higher (P < 0.001) than that before the insult. Liver histology showed collagen deposition, edema, and infiltration of inflammatory cells in livers with CCl 4 insult. Conclusion: Both longitudinal and transverse relaxation times may serve as robust markers for liver fibrosis. With the advent of single breath-hold sequences for MR relaxometry, quantitative mapping of relaxation times can be routinely and reliably performed in abdominal organs and hence may be valuable and robust in detecting liver fibrosis at early phase and monitoring its progression. © 2012 Wiley Periodicals, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/145890
ISSN
2013 Impact Factor: 2.788
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grant CouncilGRF HKU 7826/10M
Funding Information:

Contract grant sponsor: Hong Kong Research Grant Council; Contract grant number: GRF HKU 7826/10M.

References

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorChow, AMen_HK
dc.contributor.authorGao, DSen_HK
dc.contributor.authorFan, SJen_HK
dc.contributor.authorQiao, Zen_HK
dc.contributor.authorLee, FYen_HK
dc.contributor.authorYang, Jen_HK
dc.contributor.authorMan, Ken_HK
dc.contributor.authorWu, EXen_HK
dc.date.accessioned2012-03-27T09:00:47Z-
dc.date.available2012-03-27T09:00:47Z-
dc.date.issued2012en_HK
dc.identifier.citationJournal Of Magnetic Resonance Imaging, 2012, v. 36 n. 1, p. 152-158en_HK
dc.identifier.issn1053-1807en_HK
dc.identifier.urihttp://hdl.handle.net/10722/145890-
dc.description.abstractPurpose: To characterize changes in relaxation times of liver using quantitative magnetic resonance imaging (MRI) in an experimental mouse model of liver fibrosis. Quantitative MRI is a potentially robust method to characterize liver fibrosis. However, correlation between relaxation times and fibrosis stage has been controversial. Materials and Methods: Liver fibrosis was induced in male adult C57BL/6N mice (22-25 g; n = 12) by repetitive dosing of carbon tetrachloride (CCl 4). The animals were examined with a series of spin-echo (SE) images with varying TRs and multiecho SE imaging sequence at 7 T before and 2, 4, 6, and 8 weeks after CCl 4 insult. Hepatic T 1 and T 2 values were measured. Histology was performed with hematoxylin-eosin staining and Masson's trichrome staining. Results: Significant increase (P < 0.001) in hepatic T 1 was found at 2, 4, 6, and 8 weeks following CCl 4 insult as compared with that before insult. Meanwhile, hepatic T 2 at 2, 4, 6, and 8 weeks after CCl 4 insult was significantly higher (P < 0.001) than that before the insult. Liver histology showed collagen deposition, edema, and infiltration of inflammatory cells in livers with CCl 4 insult. Conclusion: Both longitudinal and transverse relaxation times may serve as robust markers for liver fibrosis. With the advent of single breath-hold sequences for MR relaxometry, quantitative mapping of relaxation times can be routinely and reliably performed in abdominal organs and hence may be valuable and robust in detecting liver fibrosis at early phase and monitoring its progression. © 2012 Wiley Periodicals, Inc.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/1053-1807/en_HK
dc.relation.ispartofJournal of Magnetic Resonance Imagingen_HK
dc.rightsJournal of Magnetic Resonance Imaging. Copyright © John Wiley & Sons, Inc.-
dc.rightsSpecial Statement for Preprint only Before publication: 'This is a preprint of an article accepted for publication in [The Journal of Pathology] Copyright © ([year]) ([Pathological Society of Great Britain and Ireland])'. After publication: the preprint notice should be amended to follows: 'This is a preprint of an article published in [include the complete citation information for the final version of the Contribution as published in the print edition of the Journal]' For Cochrane Library/ Cochrane Database of Systematic Reviews, add statement & acknowledgement : ‘This review is published as a Cochrane Review in the Cochrane Database of Systematic Reviews 20XX, Issue X. Cochrane Reviews are regularly updated as new evidence emerges and in response to comments and criticisms, and the Cochrane Database of Systematic Reviews should be consulted for the most recent version of the Review.’ Please include reference to the Review and hyperlink to the original version using the following format e.g. Authors. Title of Review. Cochrane Database of Systematic Reviews 20XX, Issue #. Art. No.: CD00XXXX. DOI: 10.1002/14651858.CD00XXXX (insert persistent link to the article by using the URL: http://dx.doi.org/10.1002/14651858.CD00XXXX) (This statement should refer to the most recent issue of the Cochrane Database of Systematic Reviews in which the Review published.)-
dc.subjectCCl 4en_HK
dc.subjectliver fibrosisen_HK
dc.subjectMRIen_HK
dc.subjectT 1, T 2 relaxometryen_HK
dc.titleMeasurement of liver T 1 and T 2 relaxation times in an experimental mouse model of liver fibrosisen_HK
dc.typeArticleen_HK
dc.identifier.emailMan, K: kwanman@hkucc.hku.hken_HK
dc.identifier.emailWu, EX: ewu1@hkucc.hku.hken_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityWu, EX=rp00193en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jmri.23606en_HK
dc.identifier.pmid22334510-
dc.identifier.scopuseid_2-s2.0-84862747595en_HK
dc.identifier.hkuros198968en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84862747595&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume36en_HK
dc.identifier.issue1en_HK
dc.identifier.spage152en_HK
dc.identifier.epage158en_HK
dc.identifier.isiWOS:000305185700014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChow, AM=16174234200en_HK
dc.identifier.scopusauthoridGao, DS=36664395800en_HK
dc.identifier.scopusauthoridFan, SJ=36514618100en_HK
dc.identifier.scopusauthoridQiao, Z=55259979400en_HK
dc.identifier.scopusauthoridLee, FY=55260303100en_HK
dc.identifier.scopusauthoridYang, J=23391308100en_HK
dc.identifier.scopusauthoridMan, K=7101754072en_HK
dc.identifier.scopusauthoridWu, EX=7202128034en_HK

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