Article: Measurement of liver T 1 and T 2 relaxation times in an experimental mouse model of liver fibrosis
| Title | Measurement of liver T 1 and T 2 relaxation times in an experimental mouse model of liver fibrosis | ||||
|---|---|---|---|---|---|
| Authors | Chow, AM1 Gao, DS1 Fan, SJ1 Qiao, Z1 Lee, FY1 Yang, J1 Man, K1 Wu, EX1 | ||||
| Keywords | CCl 4 liver fibrosis MRI T 1, T 2 relaxometry | ||||
| Issue Date | 2012 | ||||
| Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/1053-1807/ | ||||
| Citation | Journal Of Magnetic Resonance Imaging, 2012, v. 36 n. 1, p. 152-158 [How to Cite?] DOI: http://dx.doi.org/10.1002/jmri.23606 | ||||
| Abstract | Purpose: To characterize changes in relaxation times of liver using quantitative magnetic resonance imaging (MRI) in an experimental mouse model of liver fibrosis. Quantitative MRI is a potentially robust method to characterize liver fibrosis. However, correlation between relaxation times and fibrosis stage has been controversial. Materials and Methods: Liver fibrosis was induced in male adult C57BL/6N mice (22-25 g; n = 12) by repetitive dosing of carbon tetrachloride (CCl 4). The animals were examined with a series of spin-echo (SE) images with varying TRs and multiecho SE imaging sequence at 7 T before and 2, 4, 6, and 8 weeks after CCl 4 insult. Hepatic T 1 and T 2 values were measured. Histology was performed with hematoxylin-eosin staining and Masson's trichrome staining. Results: Significant increase (P < 0.001) in hepatic T 1 was found at 2, 4, 6, and 8 weeks following CCl 4 insult as compared with that before insult. Meanwhile, hepatic T 2 at 2, 4, 6, and 8 weeks after CCl 4 insult was significantly higher (P < 0.001) than that before the insult. Liver histology showed collagen deposition, edema, and infiltration of inflammatory cells in livers with CCl 4 insult. Conclusion: Both longitudinal and transverse relaxation times may serve as robust markers for liver fibrosis. With the advent of single breath-hold sequences for MR relaxometry, quantitative mapping of relaxation times can be routinely and reliably performed in abdominal organs and hence may be valuable and robust in detecting liver fibrosis at early phase and monitoring its progression. © 2012 Wiley Periodicals, Inc. | ||||
| ISSN | 1053-1807 2011 Impact Factor: 2.698 2011 SCImago Journal Rankings: 0.242 | ||||
| DOI | http://dx.doi.org/10.1002/jmri.23606 | ||||
| ISI Accession Number ID | WOS:000305185700014
Funding Information: Contract grant sponsor: Hong Kong Research Grant Council; Contract grant number: GRF HKU 7826/10M. | ||||
| References | References in Scopus |
| dc.contributor.author | Chow, AM | ||||
|---|---|---|---|---|---|
| dc.contributor.author | Gao, DS | ||||
| dc.contributor.author | Fan, SJ | ||||
| dc.contributor.author | Qiao, Z | ||||
| dc.contributor.author | Lee, FY | ||||
| dc.contributor.author | Yang, J | ||||
| dc.contributor.author | Man, K | ||||
| dc.contributor.author | Wu, EX | ||||
| dc.date.accessioned | 2012-03-27T09:00:47Z | ||||
| dc.date.available | 2012-03-27T09:00:47Z | ||||
| dc.date.issued | 2012 | ||||
| dc.description.abstract | Purpose: To characterize changes in relaxation times of liver using quantitative magnetic resonance imaging (MRI) in an experimental mouse model of liver fibrosis. Quantitative MRI is a potentially robust method to characterize liver fibrosis. However, correlation between relaxation times and fibrosis stage has been controversial. Materials and Methods: Liver fibrosis was induced in male adult C57BL/6N mice (22-25 g; n = 12) by repetitive dosing of carbon tetrachloride (CCl 4). The animals were examined with a series of spin-echo (SE) images with varying TRs and multiecho SE imaging sequence at 7 T before and 2, 4, 6, and 8 weeks after CCl 4 insult. Hepatic T 1 and T 2 values were measured. Histology was performed with hematoxylin-eosin staining and Masson's trichrome staining. Results: Significant increase (P < 0.001) in hepatic T 1 was found at 2, 4, 6, and 8 weeks following CCl 4 insult as compared with that before insult. Meanwhile, hepatic T 2 at 2, 4, 6, and 8 weeks after CCl 4 insult was significantly higher (P < 0.001) than that before the insult. Liver histology showed collagen deposition, edema, and infiltration of inflammatory cells in livers with CCl 4 insult. Conclusion: Both longitudinal and transverse relaxation times may serve as robust markers for liver fibrosis. With the advent of single breath-hold sequences for MR relaxometry, quantitative mapping of relaxation times can be routinely and reliably performed in abdominal organs and hence may be valuable and robust in detecting liver fibrosis at early phase and monitoring its progression. © 2012 Wiley Periodicals, Inc. | ||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||
| dc.identifier.citation | Journal Of Magnetic Resonance Imaging, 2012, v. 36 n. 1, p. 152-158 [How to Cite?] DOI: http://dx.doi.org/10.1002/jmri.23606 | ||||
| dc.identifier.doi | http://dx.doi.org/10.1002/jmri.23606 | ||||
| dc.identifier.epage | 158 | ||||
| dc.identifier.hkuros | 198968 | ||||
| dc.identifier.isi | WOS:000305185700014
Funding Information: Contract grant sponsor: Hong Kong Research Grant Council; Contract grant number: GRF HKU 7826/10M. | ||||
| dc.identifier.issn | 1053-1807 2011 Impact Factor: 2.698 2011 SCImago Journal Rankings: 0.242 | ||||
| dc.identifier.issue | 1 | ||||
| dc.identifier.pmid | 22334510 | ||||
| dc.identifier.scopus | eid_2-s2.0-84862747595 | ||||
| dc.identifier.spage | 152 | ||||
| dc.identifier.uri | http://hdl.handle.net/10722/145890 | ||||
| dc.identifier.volume | 36 | ||||
| dc.language | eng | ||||
| dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/1053-1807/ | ||||
| dc.publisher.place | United States | ||||
| dc.relation.ispartof | Journal of Magnetic Resonance Imaging | ||||
| dc.relation.references | References in Scopus | ||||
| dc.rights | Journal of Magnetic Resonance Imaging. Copyright © John Wiley & Sons, Inc. | ||||
| dc.rights | Special Statement for Preprint only Before publication: 'This is a preprint of an article accepted for publication in [The Journal of Pathology] Copyright © ([year]) ([Pathological Society of Great Britain and Ireland])'. After publication: the preprint notice should be amended to follows: 'This is a preprint of an article published in [include the complete citation information for the final version of the Contribution as published in the print edition of the Journal]' For Cochrane Library/ Cochrane Database of Systematic Reviews, add statement & acknowledgement : ‘This review is published as a Cochrane Review in the Cochrane Database of Systematic Reviews 20XX, Issue X. Cochrane Reviews are regularly updated as new evidence emerges and in response to comments and criticisms, and the Cochrane Database of Systematic Reviews should be consulted for the most recent version of the Review.’ Please include reference to the Review and hyperlink to the original version using the following format e.g. Authors. Title of Review. Cochrane Database of Systematic Reviews 20XX, Issue #. Art. No.: CD00XXXX. DOI: 10.1002/14651858.CD00XXXX (insert persistent link to the article by using the URL: http://dx.doi.org/10.1002/14651858.CD00XXXX) (This statement should refer to the most recent issue of the Cochrane Database of Systematic Reviews in which the Review published.) | ||||
| dc.subject | CCl 4 | ||||
| dc.subject | liver fibrosis | ||||
| dc.subject | MRI | ||||
| dc.subject | T 1, T 2 relaxometry | ||||
| dc.title | Measurement of liver T 1 and T 2 relaxation times in an experimental mouse model of liver fibrosis | ||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong