Article: Liver fibrosis: An intravoxel incoherent motion (IVIM) study
| Title | Liver fibrosis: An intravoxel incoherent motion (IVIM) study | ||||
|---|---|---|---|---|---|
| Authors | Chow, AM1 Gao, DS1 Fan, SJ1 Qiao, Z1 Lee, FY1 Yang, J1 Man, K1 Wu, EX1 | ||||
| Keywords | CCl 4 diffusion imaging IVIM liver fibrosis MRI | ||||
| Issue Date | 2012 | ||||
| Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/1053-1807/ | ||||
| Citation | Journal Of Magnetic Resonance Imaging, 2012, v. 36 n. 1, p. 159-167 [How to Cite?] DOI: http://dx.doi.org/10.1002/jmri.23607 | ||||
| Abstract | Purpose: To characterize longitudinal changes in molecular water diffusion, blood microcirculation, and their contributions to the apparent diffusion changes using intravoxel incoherent motion (IVIM) analysis in an experimental mouse model of liver fibrosis. Materials and Methods: Liver fibrosis was induced in male adult C57BL/6N mice (22-25 g; n = 12) by repetitive dosing of carbon tetrachloride (CCl 4). The respiratory-gated diffusion-weighted (DW) images were acquired using single-shot spin-echo EPI (SE-EPI) with 8 b-values and single diffusion gradient direction. True diffusion coefficient (D true), blood pseudodiffusion coefficient (D pseudo), and perfusion fraction (P fraction) were measured. Diffusion tensor imaging (DTI) was also performed for comparison. Histology was performed with hematoxylin-eosin and Masson's trichrome staining. Results: A significant decrease in D true was found at 2 weeks and 4 weeks following CCl 4 insult, as compared with that before insult. Similarly, D pseudo values before injury was significantly higher than those at 2 weeks and 4 weeks after CCl 4 insult. Meanwhile, P fraction values showed no significant differences over different timepoints. For DTI, significant decrease in ADC was observed following CCl 4 administration. Fractional anisotropy at 2 weeks after CCl 4 insult was significantly lower than that before insult, and subsequently normalized at 4 weeks after the insult. Liver histology showed collagen deposition, the presence of intracellular fat vacuoles, and cell necrosis/apoptosis in livers with CCl 4 insult. Conclusion: Both molecular water diffusion and blood microcirculation contribute to the alteration in apparent diffusion changes in liver fibrosis. Reduction in D true and D pseudo values resulted from diffusion and perfusion changes, respectively, during the progression of liver fibrosis. IVIM analysis may serve as valuable and robust tool in detecting and characterizing liver fibrosis at early stages, monitoring its progression in a noninvasive manner. © 2012 Wiley Periodicals, Inc. | ||||
| Description | This work was presented as an oral presentation at the 19th Annual Meeting of International Society of Magnetic Resonance in Medicine, Montreal, Quebec, Canada, 7–13 May, 2011. | ||||
| ISSN | 1053-1807 2011 Impact Factor: 2.698 2011 SCImago Journal Rankings: 0.242 | ||||
| DOI | http://dx.doi.org/10.1002/jmri.23607 | ||||
| ISI Accession Number ID | WOS:000305185700015
Funding Information: Contract grant sponsor: Hong Kong Research Grant Council; Contract grant number: GRF HKU 7826/10M. | ||||
| References | References in Scopus |
| dc.contributor.author | Chow, AM | ||||
|---|---|---|---|---|---|
| dc.contributor.author | Gao, DS | ||||
| dc.contributor.author | Fan, SJ | ||||
| dc.contributor.author | Qiao, Z | ||||
| dc.contributor.author | Lee, FY | ||||
| dc.contributor.author | Yang, J | ||||
| dc.contributor.author | Man, K | ||||
| dc.contributor.author | Wu, EX | ||||
| dc.date.accessioned | 2012-03-27T09:00:47Z | ||||
| dc.date.available | 2012-03-27T09:00:47Z | ||||
| dc.date.issued | 2012 | ||||
| dc.description.abstract | Purpose: To characterize longitudinal changes in molecular water diffusion, blood microcirculation, and their contributions to the apparent diffusion changes using intravoxel incoherent motion (IVIM) analysis in an experimental mouse model of liver fibrosis. Materials and Methods: Liver fibrosis was induced in male adult C57BL/6N mice (22-25 g; n = 12) by repetitive dosing of carbon tetrachloride (CCl 4). The respiratory-gated diffusion-weighted (DW) images were acquired using single-shot spin-echo EPI (SE-EPI) with 8 b-values and single diffusion gradient direction. True diffusion coefficient (D true), blood pseudodiffusion coefficient (D pseudo), and perfusion fraction (P fraction) were measured. Diffusion tensor imaging (DTI) was also performed for comparison. Histology was performed with hematoxylin-eosin and Masson's trichrome staining. Results: A significant decrease in D true was found at 2 weeks and 4 weeks following CCl 4 insult, as compared with that before insult. Similarly, D pseudo values before injury was significantly higher than those at 2 weeks and 4 weeks after CCl 4 insult. Meanwhile, P fraction values showed no significant differences over different timepoints. For DTI, significant decrease in ADC was observed following CCl 4 administration. Fractional anisotropy at 2 weeks after CCl 4 insult was significantly lower than that before insult, and subsequently normalized at 4 weeks after the insult. Liver histology showed collagen deposition, the presence of intracellular fat vacuoles, and cell necrosis/apoptosis in livers with CCl 4 insult. Conclusion: Both molecular water diffusion and blood microcirculation contribute to the alteration in apparent diffusion changes in liver fibrosis. Reduction in D true and D pseudo values resulted from diffusion and perfusion changes, respectively, during the progression of liver fibrosis. IVIM analysis may serve as valuable and robust tool in detecting and characterizing liver fibrosis at early stages, monitoring its progression in a noninvasive manner. © 2012 Wiley Periodicals, Inc. | ||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||
| dc.description | This work was presented as an oral presentation at the 19th Annual Meeting of International Society of Magnetic Resonance in Medicine, Montreal, Quebec, Canada, 7–13 May, 2011. | ||||
| dc.identifier.citation | Journal Of Magnetic Resonance Imaging, 2012, v. 36 n. 1, p. 159-167 [How to Cite?] DOI: http://dx.doi.org/10.1002/jmri.23607 | ||||
| dc.identifier.doi | http://dx.doi.org/10.1002/jmri.23607 | ||||
| dc.identifier.epage | 167 | ||||
| dc.identifier.hkuros | 198967 | ||||
| dc.identifier.hkuros | 206799 | ||||
| dc.identifier.isi | WOS:000305185700015
Funding Information: Contract grant sponsor: Hong Kong Research Grant Council; Contract grant number: GRF HKU 7826/10M. | ||||
| dc.identifier.issn | 1053-1807 2011 Impact Factor: 2.698 2011 SCImago Journal Rankings: 0.242 | ||||
| dc.identifier.issue | 1 | ||||
| dc.identifier.pmid | 22334528 | ||||
| dc.identifier.scopus | eid_2-s2.0-84862740631 | ||||
| dc.identifier.spage | 159 | ||||
| dc.identifier.uri | http://hdl.handle.net/10722/145889 | ||||
| dc.identifier.volume | 36 | ||||
| dc.language | eng | ||||
| dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/1053-1807/ | ||||
| dc.publisher.place | United States | ||||
| dc.relation.ispartof | Journal of Magnetic Resonance Imaging | ||||
| dc.relation.references | References in Scopus | ||||
| dc.rights | Journal of Magnetic Resonance Imaging. Copyright © John Wiley & Sons, Inc. | ||||
| dc.subject | CCl 4 | ||||
| dc.subject | diffusion imaging | ||||
| dc.subject | IVIM | ||||
| dc.subject | liver fibrosis | ||||
| dc.subject | MRI | ||||
| dc.title | Liver fibrosis: An intravoxel incoherent motion (IVIM) study | ||||
| dc.type | Article |
Author Affiliations
- The University of Hong Kong