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Article: Liver fibrosis: An intravoxel incoherent motion (IVIM) study

TitleLiver fibrosis: An intravoxel incoherent motion (IVIM) study
Authors
KeywordsCCl 4
diffusion imaging
IVIM
liver fibrosis
MRI
Issue Date2012
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/1053-1807/
Citation
Journal Of Magnetic Resonance Imaging, 2012, v. 36 n. 1, p. 159-167 How to Cite?
Abstract
Purpose: To characterize longitudinal changes in molecular water diffusion, blood microcirculation, and their contributions to the apparent diffusion changes using intravoxel incoherent motion (IVIM) analysis in an experimental mouse model of liver fibrosis. Materials and Methods: Liver fibrosis was induced in male adult C57BL/6N mice (22-25 g; n = 12) by repetitive dosing of carbon tetrachloride (CCl 4). The respiratory-gated diffusion-weighted (DW) images were acquired using single-shot spin-echo EPI (SE-EPI) with 8 b-values and single diffusion gradient direction. True diffusion coefficient (D true), blood pseudodiffusion coefficient (D pseudo), and perfusion fraction (P fraction) were measured. Diffusion tensor imaging (DTI) was also performed for comparison. Histology was performed with hematoxylin-eosin and Masson's trichrome staining. Results: A significant decrease in D true was found at 2 weeks and 4 weeks following CCl 4 insult, as compared with that before insult. Similarly, D pseudo values before injury was significantly higher than those at 2 weeks and 4 weeks after CCl 4 insult. Meanwhile, P fraction values showed no significant differences over different timepoints. For DTI, significant decrease in ADC was observed following CCl 4 administration. Fractional anisotropy at 2 weeks after CCl 4 insult was significantly lower than that before insult, and subsequently normalized at 4 weeks after the insult. Liver histology showed collagen deposition, the presence of intracellular fat vacuoles, and cell necrosis/apoptosis in livers with CCl 4 insult. Conclusion: Both molecular water diffusion and blood microcirculation contribute to the alteration in apparent diffusion changes in liver fibrosis. Reduction in D true and D pseudo values resulted from diffusion and perfusion changes, respectively, during the progression of liver fibrosis. IVIM analysis may serve as valuable and robust tool in detecting and characterizing liver fibrosis at early stages, monitoring its progression in a noninvasive manner. © 2012 Wiley Periodicals, Inc.
DescriptionThis work was presented as an oral presentation at the 19th Annual Meeting of International Society of Magnetic Resonance in Medicine, Montreal, Quebec, Canada, 7–13 May, 2011.
Persistent Identifierhttp://hdl.handle.net/10722/145889
ISSN
2013 Impact Factor: 2.788
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grant CouncilGRF HKU 7826/10M
Funding Information:

Contract grant sponsor: Hong Kong Research Grant Council; Contract grant number: GRF HKU 7826/10M.

References

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorChow, AMen_HK
dc.contributor.authorGao, DSen_HK
dc.contributor.authorFan, SJen_HK
dc.contributor.authorQiao, Zen_HK
dc.contributor.authorLee, FYen_HK
dc.contributor.authorYang, Jen_HK
dc.contributor.authorMan, Ken_HK
dc.contributor.authorWu, EXen_HK
dc.date.accessioned2012-03-27T09:00:47Z-
dc.date.available2012-03-27T09:00:47Z-
dc.date.issued2012en_HK
dc.identifier.citationJournal Of Magnetic Resonance Imaging, 2012, v. 36 n. 1, p. 159-167en_HK
dc.identifier.issn1053-1807en_HK
dc.identifier.urihttp://hdl.handle.net/10722/145889-
dc.descriptionThis work was presented as an oral presentation at the 19th Annual Meeting of International Society of Magnetic Resonance in Medicine, Montreal, Quebec, Canada, 7–13 May, 2011.-
dc.description.abstractPurpose: To characterize longitudinal changes in molecular water diffusion, blood microcirculation, and their contributions to the apparent diffusion changes using intravoxel incoherent motion (IVIM) analysis in an experimental mouse model of liver fibrosis. Materials and Methods: Liver fibrosis was induced in male adult C57BL/6N mice (22-25 g; n = 12) by repetitive dosing of carbon tetrachloride (CCl 4). The respiratory-gated diffusion-weighted (DW) images were acquired using single-shot spin-echo EPI (SE-EPI) with 8 b-values and single diffusion gradient direction. True diffusion coefficient (D true), blood pseudodiffusion coefficient (D pseudo), and perfusion fraction (P fraction) were measured. Diffusion tensor imaging (DTI) was also performed for comparison. Histology was performed with hematoxylin-eosin and Masson's trichrome staining. Results: A significant decrease in D true was found at 2 weeks and 4 weeks following CCl 4 insult, as compared with that before insult. Similarly, D pseudo values before injury was significantly higher than those at 2 weeks and 4 weeks after CCl 4 insult. Meanwhile, P fraction values showed no significant differences over different timepoints. For DTI, significant decrease in ADC was observed following CCl 4 administration. Fractional anisotropy at 2 weeks after CCl 4 insult was significantly lower than that before insult, and subsequently normalized at 4 weeks after the insult. Liver histology showed collagen deposition, the presence of intracellular fat vacuoles, and cell necrosis/apoptosis in livers with CCl 4 insult. Conclusion: Both molecular water diffusion and blood microcirculation contribute to the alteration in apparent diffusion changes in liver fibrosis. Reduction in D true and D pseudo values resulted from diffusion and perfusion changes, respectively, during the progression of liver fibrosis. IVIM analysis may serve as valuable and robust tool in detecting and characterizing liver fibrosis at early stages, monitoring its progression in a noninvasive manner. © 2012 Wiley Periodicals, Inc.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/1053-1807/en_HK
dc.relation.ispartofJournal of Magnetic Resonance Imagingen_HK
dc.rightsJournal of Magnetic Resonance Imaging. Copyright © John Wiley & Sons, Inc.-
dc.subjectCCl 4en_HK
dc.subjectdiffusion imagingen_HK
dc.subjectIVIMen_HK
dc.subjectliver fibrosisen_HK
dc.subjectMRIen_HK
dc.titleLiver fibrosis: An intravoxel incoherent motion (IVIM) studyen_HK
dc.typeArticleen_HK
dc.identifier.emailMan, K: kwanman@hkucc.hku.hken_HK
dc.identifier.emailWu, EX: ewu1@hkucc.hku.hken_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityWu, EX=rp00193en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jmri.23607en_HK
dc.identifier.pmid22334528-
dc.identifier.scopuseid_2-s2.0-84862740631en_HK
dc.identifier.hkuros198967en_US
dc.identifier.hkuros206799-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84862740631&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume36en_HK
dc.identifier.issue1en_HK
dc.identifier.spage159en_HK
dc.identifier.epage167en_HK
dc.identifier.isiWOS:000305185700015-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChow, AM=16174234200en_HK
dc.identifier.scopusauthoridGao, DS=36664395800en_HK
dc.identifier.scopusauthoridFan, SJ=36514618100en_HK
dc.identifier.scopusauthoridQiao, Z=55259979300en_HK
dc.identifier.scopusauthoridLee, FY=55260303800en_HK
dc.identifier.scopusauthoridYang, J=23391308100en_HK
dc.identifier.scopusauthoridMan, K=7101754072en_HK
dc.identifier.scopusauthoridWu, EX=7202128034en_HK

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