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Conference Paper: Metalloproteomics for identification of metallodrug binding proteins

TitleMetalloproteomics for identification of metallodrug binding proteins
Authors
Issue Date2011
PublisherAsia Pacific Protein Association (APPA) and the Chinese Protein Society
Citation
The 3rd Asia Pacific Protein Association (APPA) Conference with the 3rd Symposium of the Chinese Protein Society, Shanghai University, Shanghai, China, 6-9 May 2011, p. 66, abstract no. S5-003 How to Cite?
AbstractMetallomics and metalloproteomics are emerging fields addressing the molecular mechanism of metal- related life processes and the entirety of metal/metalloid species within a cell or tissue type (1,2). By comparative proteomic analysis of H. pylori cells before and after treatment with colloidal bismuth subcitrate (CBS), we found that eight proteins are significantly up- or down-regulated. Using immobilized-metal affinity chromatography (Bi- and Ni-IMAC), we isolated and subsequently identified seven bismuth-binding proteins from H. pylori extracts (3). One of the binding proteins, HspA(or GroES), was then overexpressed to validate the methodology. Recombinant H. pylori HspA was found to bind both Ni2+ and Bi3+ at its C-terminal histidine- and cysteine-rich domain of the proteins (4). Moreover, we also found that a novel zinc-binding site by negative selection induces the metallodrug susceptibility of the protein (5). Binding of the metallodrug to other histidine-rich and related proteins will also be discussed (6). This work was supported by RGC of Hong Kong (HKU7043/06P, HKU2/06C, HKU1/07C, HKU7042/07P, HKU7038/08P, HKU7049/09P, and N_HKU752/09), Croucher Foundation and the University of Hong Kong.
DescriptionSession 5: Protein interaction, network and proteomics
Protein and beyond
Persistent Identifierhttp://hdl.handle.net/10722/142677

 

DC FieldValueLanguage
dc.contributor.authorSun, Hen_US
dc.contributor.authorTsang, CNen_US
dc.contributor.authorCun, Sen_US
dc.contributor.authorHu, Len_US
dc.date.accessioned2011-10-28T02:54:15Z-
dc.date.available2011-10-28T02:54:15Z-
dc.date.issued2011en_US
dc.identifier.citationThe 3rd Asia Pacific Protein Association (APPA) Conference with the 3rd Symposium of the Chinese Protein Society, Shanghai University, Shanghai, China, 6-9 May 2011, p. 66, abstract no. S5-003en_US
dc.identifier.urihttp://hdl.handle.net/10722/142677-
dc.descriptionSession 5: Protein interaction, network and proteomics-
dc.descriptionProtein and beyond-
dc.description.abstractMetallomics and metalloproteomics are emerging fields addressing the molecular mechanism of metal- related life processes and the entirety of metal/metalloid species within a cell or tissue type (1,2). By comparative proteomic analysis of H. pylori cells before and after treatment with colloidal bismuth subcitrate (CBS), we found that eight proteins are significantly up- or down-regulated. Using immobilized-metal affinity chromatography (Bi- and Ni-IMAC), we isolated and subsequently identified seven bismuth-binding proteins from H. pylori extracts (3). One of the binding proteins, HspA(or GroES), was then overexpressed to validate the methodology. Recombinant H. pylori HspA was found to bind both Ni2+ and Bi3+ at its C-terminal histidine- and cysteine-rich domain of the proteins (4). Moreover, we also found that a novel zinc-binding site by negative selection induces the metallodrug susceptibility of the protein (5). Binding of the metallodrug to other histidine-rich and related proteins will also be discussed (6). This work was supported by RGC of Hong Kong (HKU7043/06P, HKU2/06C, HKU1/07C, HKU7042/07P, HKU7038/08P, HKU7049/09P, and N_HKU752/09), Croucher Foundation and the University of Hong Kong.-
dc.languageengen_US
dc.publisherAsia Pacific Protein Association (APPA) and the Chinese Protein Society-
dc.relation.ispartofAsia Pacific Protein Association Conferenceen_US
dc.titleMetalloproteomics for identification of metallodrug binding proteinsen_US
dc.typeConference_Paperen_US
dc.identifier.emailSun, H: hsun@hku.hken_US
dc.identifier.emailTsang, CN: cntsang@hkusua.hku.hken_US
dc.identifier.emailCun, S: shujian@hku.hken_US
dc.identifier.emailHu, L: liganghu@hku.hken_US
dc.identifier.authoritySun, H=rp00777en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros196614en_US
dc.identifier.hkuros189553-
dc.identifier.spage66, abstract no. S5-003-
dc.identifier.epage66, abstract no. S5-003-
dc.publisher.placeChina-
dc.description.otherThe 3rd Asia Pacific Protein Association (APPA) Conference with the 3rd Symposium of the Chinese Protein Society, Shanghai University, Shanghai, China, 6-9 May 2011-

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