Conference Paper: The role of adrenomedullin in tubal ectopic pregnancy

TitleThe role of adrenomedullin in tubal ectopic pregnancy
Authors
Issue Date2011
Citation
The 2011 World Congress on Reproductive Biology (WCRB), Cairns, Queensland, Australia, 9-12 October 2011. How to Cite?
AbstractAdrenomedullin (ADM), a novel vasodilator, exerts diuretic, natriuretic, angiogenic, antioxidative, anti-apoptotic and anti-inflammatory effects in various body systems. It has important regulatory functions in the reproductive system. In the female reproductive tract, ADM was highly expressed in the human and rat oviducts. ADM has been shown to regulate the ciliary beat frequency (CBF) and contractility in the oviduct, affecting the transport of spermatozoa, ova and pre-implantation embryos. The oviduct is the most frequent site of ectopic pregnancy which is an important cause of pregnancy morbidity. In this study, we investigated the role of ADM in tubal ectopic pregnancy (tEP). Oviducts from women undergoing salpingectomy for tEP and those from women undergoing hysterectomy for benign diseases were collected. The latter oviducts served as controls after treatment for 16h with estradiol, progesterone and human chorionic gonadotrophin to simulate the early first trimester hormonal milieu of human pregnancy. ADM expression was studied at the peptide and mRNA levels by immunohistochemistry and RT-PCR respectively. After treatment of the oviducts with ADM and its receptor antagonists, CBF was measured using a photometric method and the contraction of the oviduct was measured using the organ-bath technique. The tEP oviducts expressed significantly less Adm mRNA than the control. The CBF was lower in tEP oviducts and in both groups the CBF increased with graded concentrations (1-100nM) of ADM and were specifically inhibited by a hADM22-52 receptor antagonist. Treatment with ADM increased the basal tone and the frequency of contraction but decreased the amplitude in both the control and tEP groups. Contractility in both groups was not sensitive to the treatment with receptor antagonists. All the results indicate that in the oviduct, the low ADM expression, which may affect the embryo transport through ciliary beat and contraction, may be a causative factor in tubal ectopic pregnancy.
DescriptionOral: Abstract no. 124
Persistent Identifierhttp://hdl.handle.net/10722/142633

 

DC FieldValueLanguage
dc.contributor.authorO, WSen_US
dc.contributor.authorLiao, Sen_US
dc.contributor.authorLi, RHWen_US
dc.contributor.authorHo, JCMen_US
dc.contributor.authorNg, EHYen_US
dc.contributor.authorYeung, WSBen_US
dc.contributor.authorCheung, ANYen_US
dc.contributor.authorTang, Fen_US
dc.date.accessioned2011-10-28T02:53:31Z-
dc.date.available2011-10-28T02:53:31Z-
dc.date.issued2011en_US
dc.identifier.citationThe 2011 World Congress on Reproductive Biology (WCRB), Cairns, Queensland, Australia, 9-12 October 2011.en_US
dc.identifier.urihttp://hdl.handle.net/10722/142633-
dc.descriptionOral: Abstract no. 124-
dc.description.abstractAdrenomedullin (ADM), a novel vasodilator, exerts diuretic, natriuretic, angiogenic, antioxidative, anti-apoptotic and anti-inflammatory effects in various body systems. It has important regulatory functions in the reproductive system. In the female reproductive tract, ADM was highly expressed in the human and rat oviducts. ADM has been shown to regulate the ciliary beat frequency (CBF) and contractility in the oviduct, affecting the transport of spermatozoa, ova and pre-implantation embryos. The oviduct is the most frequent site of ectopic pregnancy which is an important cause of pregnancy morbidity. In this study, we investigated the role of ADM in tubal ectopic pregnancy (tEP). Oviducts from women undergoing salpingectomy for tEP and those from women undergoing hysterectomy for benign diseases were collected. The latter oviducts served as controls after treatment for 16h with estradiol, progesterone and human chorionic gonadotrophin to simulate the early first trimester hormonal milieu of human pregnancy. ADM expression was studied at the peptide and mRNA levels by immunohistochemistry and RT-PCR respectively. After treatment of the oviducts with ADM and its receptor antagonists, CBF was measured using a photometric method and the contraction of the oviduct was measured using the organ-bath technique. The tEP oviducts expressed significantly less Adm mRNA than the control. The CBF was lower in tEP oviducts and in both groups the CBF increased with graded concentrations (1-100nM) of ADM and were specifically inhibited by a hADM22-52 receptor antagonist. Treatment with ADM increased the basal tone and the frequency of contraction but decreased the amplitude in both the control and tEP groups. Contractility in both groups was not sensitive to the treatment with receptor antagonists. All the results indicate that in the oviduct, the low ADM expression, which may affect the embryo transport through ciliary beat and contraction, may be a causative factor in tubal ectopic pregnancy.-
dc.languageengen_US
dc.relation.ispartofWorld Congress on Reproductive Biology, WCRB 2011en_US
dc.titleThe role of adrenomedullin in tubal ectopic pregnancyen_US
dc.typeConference_Paperen_US
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_US
dc.identifier.emailLiao, S: lsb776@hotmail.comen_US
dc.identifier.emailLi, RHW: rhwli@HKUCC.hku.hken_US
dc.identifier.emailHo, JCM: jhocm@hku.hken_US
dc.identifier.emailNg, EHY: nghye@hku.hken_US
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_US
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_US
dc.identifier.emailTang, F: ftang@hkucc.hku.hken_US
dc.identifier.authorityO, WS=rp00315en_US
dc.identifier.authorityHo, JCM=rp00258en_US
dc.identifier.authorityNg, EHY=rp00426en_US
dc.identifier.authorityYeung, WSB=rp00331en_US
dc.identifier.authorityCheung, ANY=rp00542en_US
dc.identifier.authorityTang, F=rp00327en_US
dc.identifier.hkuros197682en_US
dc.description.otherThe 2011 World Congress on Reproductive Biology (WCRB), Cairns, Queensland, Australia, 9-12 October 2011.-

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