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Article: Stability of hepatitis B surface antigen over time: Implications for studies using stored sera

TitleStability of hepatitis B surface antigen over time: Implications for studies using stored sera
Authors
KeywordsChronic hepatitis B
HBsAg
Quantitative
Issue Date2011
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763
Citation
Journal Of Medical Virology, 2011, v. 83 n. 11, p. 1900-1904 How to Cite?
AbstractIn chronic hepatitis B, quantitative measurements of hepatitis B surface antigen (HBsAg) have been studied increasingly using stored sera to determine its usefulness in disease management. However, the stability of stored HBsAg levels has not been established. The current study determines prospectively the stability of HBsAg levels in sera stored for 1 year. Fresh sera from 105 samples were divided into 5 aliquots. The first aliquot was used to determine HBsAg at the time of blood sampling. The remaining four aliquots were stored at -20°C and thawed at 3, 6, 9, and 12 months to determine the HBsAg levels. HBsAg was measured by adopting the research protocol using the Elecsys HBsAg II assay. At baseline, age and HBV DNA showed a significant correlation with HBsAg levels (r=-0.291, P=0.003 and r=0.256, P=0.003, respectively). There was no overall significant difference observed between the HBsAg levels measured at the different time points. There was high correlation between each consecutive time points from baseline to 3 months, 3-6 months, 6-9 months, and 9-12 months (r=0.991, 0.987, 0.989, 0.993, respectively, all P<0.001). The overall log difference in HBsAg levels between baseline and at 12 months was 0.03 (range, -0.29 to 0.85), with over 99% of samples showing no significant change (defined as >1log) after 12 months. In conclusion, HBsAg levels remained stable in stored frozen sera for 12 months without significant changes, and could be used to quantify HBsAg accurately. © 2011 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/142390
ISSN
2015 Impact Factor: 1.998
2015 SCImago Journal Rankings: 1.015
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorFung, Jen_HK
dc.contributor.authorLai, CLen_HK
dc.contributor.authorYoung, Jen_HK
dc.contributor.authorWong, DKHen_HK
dc.contributor.authorYuen, Jen_HK
dc.contributor.authorSeto, WKen_HK
dc.contributor.authorYuen, MFen_HK
dc.date.accessioned2011-10-28T02:44:56Z-
dc.date.available2011-10-28T02:44:56Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Medical Virology, 2011, v. 83 n. 11, p. 1900-1904en_HK
dc.identifier.issn0146-6615en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142390-
dc.description.abstractIn chronic hepatitis B, quantitative measurements of hepatitis B surface antigen (HBsAg) have been studied increasingly using stored sera to determine its usefulness in disease management. However, the stability of stored HBsAg levels has not been established. The current study determines prospectively the stability of HBsAg levels in sera stored for 1 year. Fresh sera from 105 samples were divided into 5 aliquots. The first aliquot was used to determine HBsAg at the time of blood sampling. The remaining four aliquots were stored at -20°C and thawed at 3, 6, 9, and 12 months to determine the HBsAg levels. HBsAg was measured by adopting the research protocol using the Elecsys HBsAg II assay. At baseline, age and HBV DNA showed a significant correlation with HBsAg levels (r=-0.291, P=0.003 and r=0.256, P=0.003, respectively). There was no overall significant difference observed between the HBsAg levels measured at the different time points. There was high correlation between each consecutive time points from baseline to 3 months, 3-6 months, 6-9 months, and 9-12 months (r=0.991, 0.987, 0.989, 0.993, respectively, all P<0.001). The overall log difference in HBsAg levels between baseline and at 12 months was 0.03 (range, -0.29 to 0.85), with over 99% of samples showing no significant change (defined as >1log) after 12 months. In conclusion, HBsAg levels remained stable in stored frozen sera for 12 months without significant changes, and could be used to quantify HBsAg accurately. © 2011 Wiley-Liss, Inc.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763en_HK
dc.relation.ispartofJournal of Medical Virologyen_HK
dc.rightsJournal of Medical Virology. Copyright © John Wiley & Sons, Inc.-
dc.subjectChronic hepatitis Ben_HK
dc.subjectHBsAgen_HK
dc.subjectQuantitativeen_HK
dc.subject.meshFreezing-
dc.subject.meshHepatitis B Surface Antigens - blood - chemistry-
dc.subject.meshMiddle Aged-
dc.subject.meshProtein Stability-
dc.subject.meshSpecimen Handling - methods-
dc.titleStability of hepatitis B surface antigen over time: Implications for studies using stored seraen_HK
dc.typeArticleen_HK
dc.identifier.emailFung, J: jfung@sicklehut.comen_HK
dc.identifier.emailLai, CL: hrmelcl@hku.hken_HK
dc.identifier.emailWong, DKH: danywong@hku.hken_HK
dc.identifier.emailSeto, WK: wkseto2@hku.hken_HK
dc.identifier.emailYuen, MF: mfyuen@hku.hken_HK
dc.identifier.authorityFung, J=rp00518en_HK
dc.identifier.authorityLai, CL=rp00314en_HK
dc.identifier.authorityWong, DKH=rp00492en_HK
dc.identifier.authoritySeto, WK=rp01659en_HK
dc.identifier.authorityYuen, MF=rp00479en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jmv.22171en_HK
dc.identifier.pmid21915863-
dc.identifier.scopuseid_2-s2.0-80052738299en_HK
dc.identifier.hkuros196660en_US
dc.identifier.hkuros213673-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80052738299&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume83en_HK
dc.identifier.issue11en_HK
dc.identifier.spage1900en_HK
dc.identifier.epage1904en_HK
dc.identifier.isiWOS:000295196200005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridFung, J=23091109300en_HK
dc.identifier.scopusauthoridLai, CL=7403086396en_HK
dc.identifier.scopusauthoridYoung, J=16949957200en_HK
dc.identifier.scopusauthoridWong, DKH=7401535819en_HK
dc.identifier.scopusauthoridYuen, J=7102620480en_HK
dc.identifier.scopusauthoridSeto, WK=23390675900en_HK
dc.identifier.scopusauthoridYuen, MF=7102031955en_HK

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