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Article: A comprehensive HBV array for the detection of HBV mutants and genotype

TitleA comprehensive HBV array for the detection of HBV mutants and genotype
Authors
KeywordsAS-APEX
Drug-resistant mutations
Genotype
HBV array
Issue Date2011
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/clinbiochem
Citation
Clinical Biochemistry, 2011, v. 44 n. 14-15, p. 1253-1260 How to Cite?
AbstractObjective: To develop a comprehensive hepatitis B virus (HBV) array providing simultaneous analysis of 8 genotypes, 47 mutations of reverse-transcriptase polymerase gene and 18 mutations of S gene. Method: Oligonucleotides corresponding to various HBV-normal and -mutant sequences were spotted onto pre-treated glass slides. Single-stranded templates of the HBV gene fragment were prepared from serum-DNA of HBV-infected patients by 2-staged PCR and subjected to allele-specific arrayed-primer extension with Cy5-dCTP. Fluorescein-labelled products were scanned at 670 nm. Results: Comparative analysis of 100 unrelated samples using the array and a commercial kit, revealed 44 with additional mutations from the array, these were confirmed by sequencing. Analysis of 381 samples from 45 patients during 1-3 years of anti-viral therapy showed improved sensitivity with detection of drug-resistant mutations months before clinical relapse. The lower detection limit was 28 copies/mL. Conclusion: The array is better than many existing methods as it provides both mutations and genotype data in a single analysis. © 2011 The Canadian Society of Clinical Chemists.
Persistent Identifierhttp://hdl.handle.net/10722/142386
ISSN
2015 Impact Factor: 2.382
2015 SCImago Journal Rankings: 0.893
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, Ken_HK
dc.contributor.authorYam, Ien_HK
dc.contributor.authorYuen, Jen_HK
dc.contributor.authorYuen, MFen_HK
dc.contributor.authorLai, CLen_HK
dc.contributor.authorAlexander, GJen_HK
dc.contributor.authorChan, TKen_HK
dc.contributor.authorChan, Ven_HK
dc.date.accessioned2011-10-28T02:44:52Z-
dc.date.available2011-10-28T02:44:52Z-
dc.date.issued2011en_HK
dc.identifier.citationClinical Biochemistry, 2011, v. 44 n. 14-15, p. 1253-1260en_HK
dc.identifier.issn0009-9120en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142386-
dc.description.abstractObjective: To develop a comprehensive hepatitis B virus (HBV) array providing simultaneous analysis of 8 genotypes, 47 mutations of reverse-transcriptase polymerase gene and 18 mutations of S gene. Method: Oligonucleotides corresponding to various HBV-normal and -mutant sequences were spotted onto pre-treated glass slides. Single-stranded templates of the HBV gene fragment were prepared from serum-DNA of HBV-infected patients by 2-staged PCR and subjected to allele-specific arrayed-primer extension with Cy5-dCTP. Fluorescein-labelled products were scanned at 670 nm. Results: Comparative analysis of 100 unrelated samples using the array and a commercial kit, revealed 44 with additional mutations from the array, these were confirmed by sequencing. Analysis of 381 samples from 45 patients during 1-3 years of anti-viral therapy showed improved sensitivity with detection of drug-resistant mutations months before clinical relapse. The lower detection limit was 28 copies/mL. Conclusion: The array is better than many existing methods as it provides both mutations and genotype data in a single analysis. © 2011 The Canadian Society of Clinical Chemists.en_HK
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/clinbiochemen_HK
dc.relation.ispartofClinical Biochemistryen_HK
dc.subjectAS-APEXen_HK
dc.subjectDrug-resistant mutationsen_HK
dc.subjectGenotypeen_HK
dc.subjectHBV arrayen_HK
dc.subject.meshCarbocyaninesen_HK
dc.subject.meshDeoxycytosine Nucleotidesen_HK
dc.subject.meshDrug Resistance, Viral - geneticsen_HK
dc.subject.meshFollow-Up Studiesen_HK
dc.subject.meshGenotypeen_HK
dc.subject.meshHepatitis B - drug therapy - genetics - virologyen_HK
dc.subject.meshHepatitis B virus - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLimit of Detectionen_HK
dc.subject.meshMutationen_HK
dc.subject.meshOligonucleotide Array Sequence Analysis - methodsen_HK
dc.subject.meshPolymerase Chain Reaction - methodsen_HK
dc.subject.meshRNA-Directed DNA Polymerase - geneticsen_HK
dc.subject.meshSensitivity and Specificityen_HK
dc.subject.meshViral Envelope Proteins - geneticsen_HK
dc.titleA comprehensive HBV array for the detection of HBV mutants and genotypeen_HK
dc.typeArticleen_HK
dc.identifier.emailChan, K: kaimin@hkucc.hku.hken_HK
dc.identifier.emailYuen, MF: mfyuen@hku.hken_HK
dc.identifier.emailLai, CL: hrmelcl@hku.hken_HK
dc.identifier.emailChan, V: vnychana@hkucc.hku.hken_HK
dc.identifier.authorityChan, K=rp00489en_HK
dc.identifier.authorityYuen, MF=rp00479en_HK
dc.identifier.authorityLai, CL=rp00314en_HK
dc.identifier.authorityChan, V=rp00320en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.clinbiochem.2011.07.014en_HK
dc.identifier.pmid21851814-
dc.identifier.scopuseid_2-s2.0-80052926548en_HK
dc.identifier.hkuros196648en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80052926548&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume44en_HK
dc.identifier.issue14-15en_HK
dc.identifier.spage1253en_HK
dc.identifier.epage1260en_HK
dc.identifier.isiWOS:000295424500013-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, K=7406032228en_HK
dc.identifier.scopusauthoridYam, I=6603358817en_HK
dc.identifier.scopusauthoridYuen, J=7102620480en_HK
dc.identifier.scopusauthoridYuen, MF=7102031955en_HK
dc.identifier.scopusauthoridLai, CL=7403086396en_HK
dc.identifier.scopusauthoridAlexander, GJ=7401920548en_HK
dc.identifier.scopusauthoridChan, TK=7402687762en_HK
dc.identifier.scopusauthoridChan, V=7202654865en_HK
dc.identifier.citeulike9628930-

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