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Article: The effective fraction isolated from Radix Astragali alleviates glucose intolerance, insulin resistance and hypertriglyceridemia in db/db diabetic mice through its anti-inflammatory activity

TitleThe effective fraction isolated from Radix Astragali alleviates glucose intolerance, insulin resistance and hypertriglyceridemia in db/db diabetic mice through its anti-inflammatory activity
Authors
KeywordsAstragalus extract
Calycosin
Immunoglobulin enhancer binding protein
Hypertriglyceridemia
Insulin resistance
Issue Date2010
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.nutritionandmetabolism.com/home/
Citation
Nutrition And Metabolism, 2010, v. 7 How to Cite?
AbstractBackground: Macrophage infiltration in adipose tissue together with the aberrant production of pro-inflammatory cytokines has been identified as the key link between obesity and its related metabolic disorders. This study aims to isolate bioactive ingredients from the traditional Chinese herb Radix Astragali (Huangqi) that alleviate obesity-induced metabolic damage through inhibiting inflammation. Methods: Active fraction (Rx) that inhibits pro-inflammatory cytokine production was identified from Radix Astragali by repeated bioactivity-guided high-throughput screening. Major constituents in Rx were identified by column chromatography followed by high-performance liquid chromatography (HPLC) and mass-spectrometry. Anti-diabetic activity of Rx was evaluated in db/db mice. Results: Treatment with Rx, which included calycosin-7-β-D-glucoside (0.9%), ononin (1.2%), calycosin (4.53%) and formononetin (1.1%), significantly reduced the secretion of pro-inflammatory cytokines (TNF-α, IL-6 and MCP-1) in human THP-1 macrophages and lipopolysaccharide (LPS)-induced activation of NF-B in mouse RAW-Blue macrophages in a dose-dependent manner. Chronic administration of Rx in db/db obese mice markedly decreased the levels of both fed and fasting glucose, reduced serum triglyceride, and also alleviated insulin resistance and glucose intolerance when compared to vehicle-treated controls. The mRNA expression levels of inflammatory cell markers CD68 and F4/80, and cytokines MCP-1, TNF-α and IL-6 were significantly reduced in epididymal adipose tissue while the alternatively activated macrophage marker arginase I was markedly increased in the Rx-treated mice. Conclusion: These findings suggest that suppression of the inflammation pathways in macrophages represents a valid strategy for high-throughput screening of lead compounds with anti-diabetic and insulin sensitizing properties, and further support the etiological role of inflammation in the pathogenesis of obesity-related metabolic disorders. © 2010 Hoo et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/142383
ISSN
2015 Impact Factor: 3.28
2015 SCImago Journal Rankings: 1.638
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Jockey Club Charities Trust (HKU)JCICM-P2-05
Funding Information:

This work was funded by The Hong Kong Jockey Club Charities Trust (JCICM-P2-05 (HKU)).

References

 

DC FieldValueLanguage
dc.contributor.authorHoo, RLCen_HK
dc.contributor.authorWong, JYLen_HK
dc.contributor.authorQiao, CFen_HK
dc.contributor.authorXu, Aen_HK
dc.contributor.authorXu, HXen_HK
dc.contributor.authorLam, KSLen_HK
dc.date.accessioned2011-10-28T02:44:45Z-
dc.date.available2011-10-28T02:44:45Z-
dc.date.issued2010en_HK
dc.identifier.citationNutrition And Metabolism, 2010, v. 7en_HK
dc.identifier.issn1743-7075en_HK
dc.identifier.urihttp://hdl.handle.net/10722/142383-
dc.description.abstractBackground: Macrophage infiltration in adipose tissue together with the aberrant production of pro-inflammatory cytokines has been identified as the key link between obesity and its related metabolic disorders. This study aims to isolate bioactive ingredients from the traditional Chinese herb Radix Astragali (Huangqi) that alleviate obesity-induced metabolic damage through inhibiting inflammation. Methods: Active fraction (Rx) that inhibits pro-inflammatory cytokine production was identified from Radix Astragali by repeated bioactivity-guided high-throughput screening. Major constituents in Rx were identified by column chromatography followed by high-performance liquid chromatography (HPLC) and mass-spectrometry. Anti-diabetic activity of Rx was evaluated in db/db mice. Results: Treatment with Rx, which included calycosin-7-β-D-glucoside (0.9%), ononin (1.2%), calycosin (4.53%) and formononetin (1.1%), significantly reduced the secretion of pro-inflammatory cytokines (TNF-α, IL-6 and MCP-1) in human THP-1 macrophages and lipopolysaccharide (LPS)-induced activation of NF-B in mouse RAW-Blue macrophages in a dose-dependent manner. Chronic administration of Rx in db/db obese mice markedly decreased the levels of both fed and fasting glucose, reduced serum triglyceride, and also alleviated insulin resistance and glucose intolerance when compared to vehicle-treated controls. The mRNA expression levels of inflammatory cell markers CD68 and F4/80, and cytokines MCP-1, TNF-α and IL-6 were significantly reduced in epididymal adipose tissue while the alternatively activated macrophage marker arginase I was markedly increased in the Rx-treated mice. Conclusion: These findings suggest that suppression of the inflammation pathways in macrophages represents a valid strategy for high-throughput screening of lead compounds with anti-diabetic and insulin sensitizing properties, and further support the etiological role of inflammation in the pathogenesis of obesity-related metabolic disorders. © 2010 Hoo et al; licensee BioMed Central Ltd.en_HK
dc.languageengen_US
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.nutritionandmetabolism.com/home/en_HK
dc.relation.ispartofNutrition and Metabolismen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsNutrition & Metabolism. Copyright © BioMed Central Ltd.-
dc.subjectAstragalus extract-
dc.subjectCalycosin-
dc.subjectImmunoglobulin enhancer binding protein-
dc.subjectHypertriglyceridemia-
dc.subjectInsulin resistance-
dc.titleThe effective fraction isolated from Radix Astragali alleviates glucose intolerance, insulin resistance and hypertriglyceridemia in db/db diabetic mice through its anti-inflammatory activityen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1743-7075&volume=7 article no. 67&spage=&epage=&date=2010&atitle=The+effective+fraction+isolated+from+Radix+Astragali+alleviates+glucose+intolerance,+insulin+resistance+and+hypertriglyceridemia+in+db/db+diabetic+mice+through+its+anti-inflammatory+activity-
dc.identifier.emailHoo, RLC:rubyhoo@hkucc.hku.hken_HK
dc.identifier.emailXu, A:amxu@hkucc.hku.hken_HK
dc.identifier.emailLam, KSL:ksllam@hku.hken_HK
dc.identifier.authorityHoo, RLC=rp01334en_HK
dc.identifier.authorityXu, A=rp00485en_HK
dc.identifier.authorityLam, KSL=rp00343en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1743-7075-7-67en_HK
dc.identifier.pmid20735814-
dc.identifier.pmcidPMC2936435-
dc.identifier.scopuseid_2-s2.0-77955793729en_HK
dc.identifier.hkuros184655en_US
dc.identifier.hkuros226352-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77955793729&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.spage67en_HK
dc.identifier.isiWOS:000282491800001-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridHoo, RLC=6602369766en_HK
dc.identifier.scopusauthoridWong, JYL=36471364800en_HK
dc.identifier.scopusauthoridQiao, CF=9746680900en_HK
dc.identifier.scopusauthoridXu, A=7202655409en_HK
dc.identifier.scopusauthoridXu, HX=23483009300en_HK
dc.identifier.scopusauthoridLam, KSL=8082870600en_HK
dc.identifier.citeulike7771724-

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