File Download

There are no files associated with this item.

Supplementary

Conference Paper: Developmental plasticity of GABAergic synapses in medial vestibular nucleus of rats

TitleDevelopmental plasticity of GABAergic synapses in medial vestibular nucleus of rats
Authors
KeywordsSynaptic Plasticity
Vestibular
GABAA Receptor
Issue Date2010
PublisherSociety for Neuroscience.
Citation
The 40th Annual Meeting of the Society for Neuroscience (SfN 2010), San Diego, California, USA, 13-17 November 2010 How to Cite?
AbstractInhibitory synaptic transmission within the vestibular nucleus is known to play a key regulatory role in coordinating central vestibular functions. To investigate the maturational changes of GABAergic transmission in medial vestibular nucleus, whole-cell patch-clamp experiments were conducted in brainstem slices of postnatal Sprague Dawley rats. Spontaneous inhibitory postsynaptic currents (sIPSCs) in medial vestibular neurons were isolated in the presence of ionotropic glutamate receptor antagonists (D-AP5 and CNQX). That these currents were abolished by GABAA receptor antagonist bicuculline indicates that the sIPSCs were mediated by GABAA receptor. The sIPSCs exhibited a gradual increase in frequency during the first two postnatal weeks and reached a plateau thereafter. Their amplitudes however did not change with postnatal development. To further investigate whether GABA receptors contribute to synaptic plasticity in the developing vestibular nucleus, a protocol consisting of repetitive depolarizing pulses at 0.1 Hz was used. Such stimuli did not induce any change in the amplitude of sIPSCs. Between P3 and P7, a majority of these GABAA receptor-mediated sIPSCs exhibited long-lasting frequency decrease after repetitive stimulation. From P14 to P22, however, only a small proportion of these sIPSCs showed such a long-lasting frequency decrease while the remaining neurons showed no change in frequency after repetitive stimulation. Taken together, we have demonstrated that long-term changes in GABAergic synaptic efficacy could be elicited in medial vestibular neurons during a limited time window of postnatal development.
DescriptionPoster Session 141: LTD
Program#141.7 & Poster#F44
Fulltext of the abstract in: http://www.abstractsonline.com/Plan/ViewAbstract.aspx?mID=2554&sKey=9f08ef47-8440-40ad-8cac-5e133f5cbc9b&cKey=0bd7ebe8-2b7b-433c-832b-ce39b664e7b1&mKey=e5d5c83f-ce2d-4d71-9dd6-fc7231e090fb
Persistent Identifierhttp://hdl.handle.net/10722/140518

 

DC FieldValueLanguage
dc.contributor.authorHu, Hen_US
dc.contributor.authorLai, SKen_US
dc.contributor.authorLai, CHen_US
dc.contributor.authorShum, DKYen_US
dc.contributor.authorChan, YSen_US
dc.date.accessioned2011-09-23T06:13:48Z-
dc.date.available2011-09-23T06:13:48Z-
dc.date.issued2010en_US
dc.identifier.citationThe 40th Annual Meeting of the Society for Neuroscience (SfN 2010), San Diego, California, USA, 13-17 November 2010en_US
dc.identifier.urihttp://hdl.handle.net/10722/140518-
dc.descriptionPoster Session 141: LTD-
dc.descriptionProgram#141.7 & Poster#F44-
dc.descriptionFulltext of the abstract in: http://www.abstractsonline.com/Plan/ViewAbstract.aspx?mID=2554&sKey=9f08ef47-8440-40ad-8cac-5e133f5cbc9b&cKey=0bd7ebe8-2b7b-433c-832b-ce39b664e7b1&mKey=e5d5c83f-ce2d-4d71-9dd6-fc7231e090fb-
dc.description.abstractInhibitory synaptic transmission within the vestibular nucleus is known to play a key regulatory role in coordinating central vestibular functions. To investigate the maturational changes of GABAergic transmission in medial vestibular nucleus, whole-cell patch-clamp experiments were conducted in brainstem slices of postnatal Sprague Dawley rats. Spontaneous inhibitory postsynaptic currents (sIPSCs) in medial vestibular neurons were isolated in the presence of ionotropic glutamate receptor antagonists (D-AP5 and CNQX). That these currents were abolished by GABAA receptor antagonist bicuculline indicates that the sIPSCs were mediated by GABAA receptor. The sIPSCs exhibited a gradual increase in frequency during the first two postnatal weeks and reached a plateau thereafter. Their amplitudes however did not change with postnatal development. To further investigate whether GABA receptors contribute to synaptic plasticity in the developing vestibular nucleus, a protocol consisting of repetitive depolarizing pulses at 0.1 Hz was used. Such stimuli did not induce any change in the amplitude of sIPSCs. Between P3 and P7, a majority of these GABAA receptor-mediated sIPSCs exhibited long-lasting frequency decrease after repetitive stimulation. From P14 to P22, however, only a small proportion of these sIPSCs showed such a long-lasting frequency decrease while the remaining neurons showed no change in frequency after repetitive stimulation. Taken together, we have demonstrated that long-term changes in GABAergic synaptic efficacy could be elicited in medial vestibular neurons during a limited time window of postnatal development.-
dc.languageengen_US
dc.publisherSociety for Neuroscience.-
dc.relation.ispartofNeuroscience 2010-
dc.rightsNeuroscience 2010. Copyright © Society for Neuroscience.-
dc.subjectSynaptic Plasticity-
dc.subjectVestibular-
dc.subjectGABAA Receptor-
dc.titleDevelopmental plasticity of GABAergic synapses in medial vestibular nucleus of ratsen_US
dc.typeConference_Paperen_US
dc.identifier.emailLai, SK: estherlai@hkusua.hku.hken_US
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_US
dc.identifier.emailChan, YS: yschan@hku.hken_US
dc.identifier.authorityShum, DKY=rp00321en_US
dc.identifier.authorityChan, YS=rp00318en_US
dc.identifier.hkuros195358en_US
dc.publisher.placeUnited States-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats