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Conference Paper: Circulating plasma pigment epithelium-derived factor (PEDF) levels are associated with insulin resistance in women with estrogen deficiency
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TitleCirculating plasma pigment epithelium-derived factor (PEDF) levels are associated with insulin resistance in women with estrogen deficiency
 
AuthorsYuen, MM
Chow, WS
Cheng, C
Pun, TC
Law, LSC
Tso, AWK
Xu, A
Lam, KSL
 
Issue Date2011
 
PublisherAmerican Diabetes Association.
 
CitationThe 71st American Diabetes Association Scientific Sessions, San Diego, CA., 24-28 June 2011. [How to Cite?]
 
AbstractPigment epithelium-derived factor (PEDF) is implicated in murine insulin resistance and metabolic dysfunctions. Women have lower serum PEDF levels and 17β-estradiol inhibits the transcription of PEDF in human ovarian epithelial cells. To investigate whether estradiol regulates PEDF in vivo, we studied the changes in PEDF levels in 21 pre-menopausal women (age = 49.6 ± 3.5 years) following bilateral oophorectomy for benign gynaecological conditions. Body mass index (BMI), waist circumference (WC), percentage body fat, and plasma PEDF (ng/ml) and estradiol (pmol/l) levels were measured before and after oophorectomy. The changes (Δ) in PEDF, estradiol, homeostatic model assessment of insulin resistance (HOMA-IR) and quantitative insulin-sensitivity check index (QUICKI) were analyzed with repeated measure ANOVA. The mean duration between pre- and post-operative assessment was 4.0±0.7 months. BMI was marginally reduced in the post-operative period (25.2 ± 4.0 kg/m2 pre-op vs. 24.8 ± 4.3 kg/m2 post-op; p = 0.048). There were no significant changes in WC or percentage body fat The marked post-operative reduction in plasma estradiol levels (225 [109-418] pmol/l pre-op vs. 32 [19-50] pmol/l post-op; p < 0.001) was associated with increases in plasma PEDF levels (7.7 ± 1.6 ng/ml pre-op vs. 8.8 ± 1.5 ng/ml post-op; p = 0.001). An inverse relationship was found between Δestradiol and ΔPEDF (r = -0.497; p = 0.022). Insulin resistance increased post-operatively, as indicated by HOMA-IR (1.06 [0.73-1.93] pre-op vs. 1.63 [1.06-2.26] post-op; p = 0.002) and QUICKI (0.38 ± 0.04 pre-op vs. 0.36 ± 0.03 post-op; p = 0.006). ΔHOMA-IR and ΔQUICKI became insignificant (p = 0.101 and 0.141 respectively) after adjusting for ΔPEDF. In conclusion, the reduction in plasma estradiol levels following oophorectomy was significantly associated with increases in plasma PEDF levels and insulin resistance. Our data suggest that estradiol is a physiological regulator of PEDF expression in humans and that an increase in circulating PEDF contributes to the increase in insulin resistance in these women with estrogen deficiency following bilateral oophorectomy.
 
DescriptionPoster Presentation: abstract no. 1780-P
 
DC FieldValue
dc.contributor.authorYuen, MM
 
dc.contributor.authorChow, WS
 
dc.contributor.authorCheng, C
 
dc.contributor.authorPun, TC
 
dc.contributor.authorLaw, LSC
 
dc.contributor.authorTso, AWK
 
dc.contributor.authorXu, A
 
dc.contributor.authorLam, KSL
 
dc.date.accessioned2011-09-23T06:11:53Z
 
dc.date.available2011-09-23T06:11:53Z
 
dc.date.issued2011
 
dc.description.abstractPigment epithelium-derived factor (PEDF) is implicated in murine insulin resistance and metabolic dysfunctions. Women have lower serum PEDF levels and 17β-estradiol inhibits the transcription of PEDF in human ovarian epithelial cells. To investigate whether estradiol regulates PEDF in vivo, we studied the changes in PEDF levels in 21 pre-menopausal women (age = 49.6 ± 3.5 years) following bilateral oophorectomy for benign gynaecological conditions. Body mass index (BMI), waist circumference (WC), percentage body fat, and plasma PEDF (ng/ml) and estradiol (pmol/l) levels were measured before and after oophorectomy. The changes (Δ) in PEDF, estradiol, homeostatic model assessment of insulin resistance (HOMA-IR) and quantitative insulin-sensitivity check index (QUICKI) were analyzed with repeated measure ANOVA. The mean duration between pre- and post-operative assessment was 4.0±0.7 months. BMI was marginally reduced in the post-operative period (25.2 ± 4.0 kg/m2 pre-op vs. 24.8 ± 4.3 kg/m2 post-op; p = 0.048). There were no significant changes in WC or percentage body fat The marked post-operative reduction in plasma estradiol levels (225 [109-418] pmol/l pre-op vs. 32 [19-50] pmol/l post-op; p < 0.001) was associated with increases in plasma PEDF levels (7.7 ± 1.6 ng/ml pre-op vs. 8.8 ± 1.5 ng/ml post-op; p = 0.001). An inverse relationship was found between Δestradiol and ΔPEDF (r = -0.497; p = 0.022). Insulin resistance increased post-operatively, as indicated by HOMA-IR (1.06 [0.73-1.93] pre-op vs. 1.63 [1.06-2.26] post-op; p = 0.002) and QUICKI (0.38 ± 0.04 pre-op vs. 0.36 ± 0.03 post-op; p = 0.006). ΔHOMA-IR and ΔQUICKI became insignificant (p = 0.101 and 0.141 respectively) after adjusting for ΔPEDF. In conclusion, the reduction in plasma estradiol levels following oophorectomy was significantly associated with increases in plasma PEDF levels and insulin resistance. Our data suggest that estradiol is a physiological regulator of PEDF expression in humans and that an increase in circulating PEDF contributes to the increase in insulin resistance in these women with estrogen deficiency following bilateral oophorectomy.
 
dc.descriptionPoster Presentation: abstract no. 1780-P
 
dc.identifier.citationThe 71st American Diabetes Association Scientific Sessions, San Diego, CA., 24-28 June 2011. [How to Cite?]
 
dc.identifier.hkuros194986
 
dc.identifier.urihttp://hdl.handle.net/10722/140445
 
dc.languageeng
 
dc.publisherAmerican Diabetes Association.
 
dc.publisher.placeUnited States
 
dc.relation.ispartofAmerican Diabetes Association Scientific Sessions, 2011
 
dc.rightsThis is an author-created, uncopyedited electronic version of an article accepted for publication in TITLE [Journal URL] The American Diabetes Association (ADA), publisher of TITLE, is not responsible for any errors or omissions in this version of the manuscript or any version derived from it by third parties. The definitive publisher-authenticated version is available online at [URL]
 
dc.titleCirculating plasma pigment epithelium-derived factor (PEDF) levels are associated with insulin resistance in women with estrogen deficiency
 
dc.typeConference_Paper
 
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<item><contributor.author>Yuen, MM</contributor.author>
<contributor.author>Chow, WS</contributor.author>
<contributor.author>Cheng, C</contributor.author>
<contributor.author>Pun, TC</contributor.author>
<contributor.author>Law, LSC</contributor.author>
<contributor.author>Tso, AWK</contributor.author>
<contributor.author>Xu, A</contributor.author>
<contributor.author>Lam, KSL</contributor.author>
<date.accessioned>2011-09-23T06:11:53Z</date.accessioned>
<date.available>2011-09-23T06:11:53Z</date.available>
<date.issued>2011</date.issued>
<identifier.citation>The 71st American Diabetes Association Scientific Sessions, San Diego, CA., 24-28 June 2011.</identifier.citation>
<identifier.uri>http://hdl.handle.net/10722/140445</identifier.uri>
<description>Poster Presentation: abstract no. 1780-P</description>
<description.abstract>Pigment epithelium-derived factor (PEDF) is implicated in murine insulin resistance and metabolic dysfunctions. Women have lower serum PEDF levels and 17&#946;-estradiol inhibits the transcription of PEDF in human ovarian epithelial cells. To investigate whether estradiol regulates PEDF in vivo, we studied the changes in PEDF levels in 21 pre-menopausal women (age = 49.6 &#177; 3.5 years) following bilateral oophorectomy for benign gynaecological conditions. Body mass index (BMI), waist circumference (WC), percentage body fat, and plasma PEDF (ng/ml) and estradiol (pmol/l) levels were measured before and after oophorectomy. The changes (&#916;) in PEDF, estradiol, homeostatic model assessment of insulin resistance (HOMA-IR) and quantitative insulin-sensitivity check index (QUICKI) were analyzed with repeated measure ANOVA. The mean duration between pre- and post-operative assessment was 4.0&#177;0.7 months. BMI was marginally reduced in the post-operative period (25.2 &#177; 4.0 kg/m2 pre-op vs. 24.8 &#177; 4.3 kg/m2 post-op; p = 0.048). There were no significant changes in WC or percentage body fat The marked post-operative reduction in plasma estradiol levels (225 [109-418] pmol/l pre-op vs. 32 [19-50] pmol/l post-op; p &lt; 0.001) was associated with increases in plasma PEDF levels (7.7 &#177; 1.6 ng/ml pre-op vs. 8.8 &#177; 1.5 ng/ml post-op; p = 0.001). An inverse relationship was found between &#916;estradiol and &#916;PEDF (r = -0.497; p = 0.022). Insulin resistance increased post-operatively, as indicated by HOMA-IR (1.06 [0.73-1.93] pre-op vs. 1.63 [1.06-2.26] post-op; p = 0.002) and QUICKI (0.38 &#177; 0.04 pre-op vs. 0.36 &#177; 0.03 post-op; p = 0.006). &#916;HOMA-IR and &#916;QUICKI became insignificant (p = 0.101 and 0.141 respectively) after adjusting for &#916;PEDF. In conclusion, the reduction in plasma estradiol levels following oophorectomy was significantly associated with increases in plasma PEDF levels and insulin resistance. Our data suggest that estradiol is a physiological regulator of PEDF expression in humans and that an increase in circulating PEDF contributes to the increase in insulin resistance in these women with estrogen deficiency following bilateral oophorectomy.</description.abstract>
<language>eng</language>
<publisher>American Diabetes Association.</publisher>
<relation.ispartof>American Diabetes Association Scientific Sessions, 2011</relation.ispartof>
<rights>This is an author-created, uncopyedited electronic version of an article accepted for publication in TITLE [Journal URL] The American Diabetes Association (ADA), publisher of TITLE, is not responsible for any errors or omissions in this version of the manuscript or any version derived from it by third parties. The definitive publisher-authenticated version is available online at [URL]</rights>
<title>Circulating plasma pigment epithelium-derived factor (PEDF) levels are associated with insulin resistance in women with estrogen deficiency</title>
<type>Conference_Paper</type>
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<publisher.place>United States</publisher.place>
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