File Download

There are no files associated with this item.

Conference Paper: Characterization of human mesenchymal stem cells selected by collagen barrier

TitleCharacterization of human mesenchymal stem cells selected by collagen barrier
Authors
Issue Date2011
Citation
The 2011 Annual Meeting of the Asia Pacific Chapter of the Tissue Engineering and Regenerative Medicine International Society (TERMIS), Waterfront Conference Centre, Singapore, 3-5 August 2011. In Proceedings of the TERMIS Asia Pacific Meeting, 2011, p. 182 How to Cite?
AbstractBACKGROUND: Stem cell therapy is a promising approach for tissue regeneration but there exists a problem of low engraftment rate to the injury site. Our laboratory has shown that human mesenchymal stem cells selected by collagen barrier (3D migrated cells) have higher migration capacity than those remained (3D remained cells). These 3D migrated cells might be hopeful candidate for improving engraftment efficacy. In order to investigate the underlying cause of the superior migratory activities of the selected cells, the cell-extracellular matrix-interacting molecules including matrix metalloproteinases (MMP) and integrins will be characterized in this study. METHODS: Human mesenchymal stem cells were encapsulated as collagen microspheres and allowed to migrate out via plating. Unplated microspheres and unencapsulated cells serve as three-dimensional (3D control) and two-dimensional (2D control) cell culture controls. These cells were characterized with MMP-1, -2, -9, -13, -14 and integrin alpha-2, alpha-v, beta-1 and beta-3 secretion and expression profile. Self-renewal assay, MTT assay for proliferative activity and multiple differentiation potential of these cells were also carried out to ensure their stem cell properties. RESULTS: 3D migrated cells secrete more MMP-1 than other experimental groups and less MMP-2 than 3D remained cells. 3D migrated cells have similar self-renewal capacity with 2D control, but higher proliferative activity than other groups in MTT assay in 72 hrs. CONCLUSION: 3D migrated cells have a different MMP secretion profile from other experimental groups, with higher proliferation rate and without compromising their self-renewing capacity.
DescriptionPoster Presentation
Persistent Identifierhttp://hdl.handle.net/10722/140128

 

DC FieldValueLanguage
dc.contributor.authorWong, YKen_US
dc.contributor.authorChan, SCHen_US
dc.contributor.authorShum, Den_US
dc.contributor.authorChan, GCFen_US
dc.contributor.authorChan, BPen_US
dc.date.accessioned2011-09-23T06:06:56Z-
dc.date.available2011-09-23T06:06:56Z-
dc.date.issued2011en_US
dc.identifier.citationThe 2011 Annual Meeting of the Asia Pacific Chapter of the Tissue Engineering and Regenerative Medicine International Society (TERMIS), Waterfront Conference Centre, Singapore, 3-5 August 2011. In Proceedings of the TERMIS Asia Pacific Meeting, 2011, p. 182en_US
dc.identifier.urihttp://hdl.handle.net/10722/140128-
dc.descriptionPoster Presentation-
dc.description.abstractBACKGROUND: Stem cell therapy is a promising approach for tissue regeneration but there exists a problem of low engraftment rate to the injury site. Our laboratory has shown that human mesenchymal stem cells selected by collagen barrier (3D migrated cells) have higher migration capacity than those remained (3D remained cells). These 3D migrated cells might be hopeful candidate for improving engraftment efficacy. In order to investigate the underlying cause of the superior migratory activities of the selected cells, the cell-extracellular matrix-interacting molecules including matrix metalloproteinases (MMP) and integrins will be characterized in this study. METHODS: Human mesenchymal stem cells were encapsulated as collagen microspheres and allowed to migrate out via plating. Unplated microspheres and unencapsulated cells serve as three-dimensional (3D control) and two-dimensional (2D control) cell culture controls. These cells were characterized with MMP-1, -2, -9, -13, -14 and integrin alpha-2, alpha-v, beta-1 and beta-3 secretion and expression profile. Self-renewal assay, MTT assay for proliferative activity and multiple differentiation potential of these cells were also carried out to ensure their stem cell properties. RESULTS: 3D migrated cells secrete more MMP-1 than other experimental groups and less MMP-2 than 3D remained cells. 3D migrated cells have similar self-renewal capacity with 2D control, but higher proliferative activity than other groups in MTT assay in 72 hrs. CONCLUSION: 3D migrated cells have a different MMP secretion profile from other experimental groups, with higher proliferation rate and without compromising their self-renewing capacity.-
dc.languageengen_US
dc.relation.ispartofProceedings of the TERMIS Asia Pacific Meetingen_US
dc.titleCharacterization of human mesenchymal stem cells selected by collagen barrieren_US
dc.typeConference_Paperen_US
dc.identifier.emailChan, SCH: bcsty99@hkucc.hku.hken_US
dc.identifier.emailShum, D: shumdkhk@hkucc.hku.hken_US
dc.identifier.emailChan, GCF: gcfchan@hku.hken_US
dc.identifier.emailChan, BP: bpchan@hkucc.hku.hken_US
dc.identifier.authorityShum, D=rp00321en_US
dc.identifier.authorityChan, GCF=rp00431en_US
dc.identifier.authorityChan, BP=rp00087en_US
dc.identifier.hkuros196507en_US
dc.identifier.spage182en_US
dc.identifier.epage182en_US
dc.description.otherThe 2011 Annual Meeting of the Asia Pacific Chapter of the Tissue Engineering and Regenerative Medicine International Society (TERMIS), Waterfront Conference Centre, Singapore, 3-5 August 2011. In Proceedings of the TERMIS Asia Pacific Meeting, 2011, p. 182-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats