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Conference Paper: Functional significance of the 3’-UTR in the mRNA of the stress-inducible protein CHOP/Gadd153

TitleFunctional significance of the 3’-UTR in the mRNA of the stress-inducible protein CHOP/Gadd153
Authors
Issue Date2011
Citation
The 2011 Hong Kong Inter-University Biochemistry Postgraduate Symposium, The University of Hong Kong, Hong Kong, 11 June 2011. How to Cite?
AbstractHow translation is regulated by the 3’-untranslated region (3’-UTR) of many messenger RNA is presently not fully understood. This issue is addressed in this study with regard to those mRNA species that are specifically expressed in stressed cells, as exemplified by the stress-inducible CHOP/Gadd153 mRNA. A human genomic fragment with DNA sequence corresponding to the entire 3’-UTR plus an additional 500bp immediately downstream from the polyadenylation site of CHOP/Gadd153 mRNA was cloned into the pEGFP-C1 plasmid. The transcription of this pEGFP-CHOP plasmid in HeLa cells produced two mRNA transcripts due to utilization of either the polyA-signal of the CHOP/Gadd153 gene or the pEGFP-C1 plasmid itself. The EGFP protein level in the pEGFP-CHOP transfected cells was several-fold lower than cells expressing the control (pEGFP-C1). The deletion of the first 171bp from the 5’-end of the ...
DescriptionPoster Presentation: no. P-H041
The Conference program's website is located at http://www.biochem.hku.hk/postgraduate/symposium2011/
Persistent Identifierhttp://hdl.handle.net/10722/140092

 

DC FieldValueLanguage
dc.contributor.authorLui, WTen_US
dc.contributor.authorWong, NSen_US
dc.date.accessioned2011-09-23T06:06:31Z-
dc.date.available2011-09-23T06:06:31Z-
dc.date.issued2011en_US
dc.identifier.citationThe 2011 Hong Kong Inter-University Biochemistry Postgraduate Symposium, The University of Hong Kong, Hong Kong, 11 June 2011.en_US
dc.identifier.urihttp://hdl.handle.net/10722/140092-
dc.descriptionPoster Presentation: no. P-H041-
dc.descriptionThe Conference program's website is located at http://www.biochem.hku.hk/postgraduate/symposium2011/-
dc.description.abstractHow translation is regulated by the 3’-untranslated region (3’-UTR) of many messenger RNA is presently not fully understood. This issue is addressed in this study with regard to those mRNA species that are specifically expressed in stressed cells, as exemplified by the stress-inducible CHOP/Gadd153 mRNA. A human genomic fragment with DNA sequence corresponding to the entire 3’-UTR plus an additional 500bp immediately downstream from the polyadenylation site of CHOP/Gadd153 mRNA was cloned into the pEGFP-C1 plasmid. The transcription of this pEGFP-CHOP plasmid in HeLa cells produced two mRNA transcripts due to utilization of either the polyA-signal of the CHOP/Gadd153 gene or the pEGFP-C1 plasmid itself. The EGFP protein level in the pEGFP-CHOP transfected cells was several-fold lower than cells expressing the control (pEGFP-C1). The deletion of the first 171bp from the 5’-end of the ...-
dc.languageengen_US
dc.relation.ispartofHong Kong Inter-University Biochemistry Postgraduate Symposium 2011en_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleFunctional significance of the 3’-UTR in the mRNA of the stress-inducible protein CHOP/Gadd153en_US
dc.typeConference_Paperen_US
dc.identifier.emailWong, NS: nswong@hku.hken_US
dc.identifier.authorityWong, NS=rp00340en_US
dc.description.naturepostprint-
dc.identifier.hkuros192881en_US

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