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Article: Circadian rhythm of circulating fibroblast growth factor 21 is related to diurnal changes in fatty acids in humans

TitleCircadian rhythm of circulating fibroblast growth factor 21 is related to diurnal changes in fatty acids in humans
Authors
Issue Date2011
PublisherAmerican Association for Clinical Chemistry, Inc. The Journal's web site is located at http://www.clinchem.org
Citation
Clinical Chemistry, 2011, v. 57 n. 5, p. 691-700 How to Cite?
AbstractBACKGROUND: Fibroblast growth factor (FGF) 21 is an endocrine factor actively involved in glucose and lipid metabolism in rodents. However, little is known about its physiological function and regulation in humans. This study investigated the diurnal changes in circulating FGF21 concentrations and their association with other metabolic markers in both obese and lean individuals. METHODS: A total of 36 volunteers were assigned to 2 groups. One group received 3 standardized meals and another group was fasted for 24 h. Blood samples were drawn every 30 min throughout a 24-h period. Circulating FGF21 concentrations were measured with an in-house chemiluminescence immunoassay. The effects of fatty acids on hepatic production of FGF21 were determined by using real-time PCR. RESULTS: In both the fasting and standardized meals groups, circulating FGF21 began to rise at midnight, reaching a peak in the early morning and then declining to basal concentrations early in the afternoon. Baseline concentrations of circulating FGF21 were much higher in obese individuals than in lean individuals (P < 0.05). However, the magnitude of the nocturnal rise in circulating FGF21 was significantly blunted in obese individuals. The 24-h oscillatory pattern of circulating FGF21 resembled that of free fatty acids and cortisol, but was opposite to the patterns of insulin and glucose. Unsaturated fatty acids induced time-dependent expression of FGF21 mRNA in human hepatocytes. CONCLUSIONS: These findings support the role of FGF21 as an important metabolic regulator that integrates the circadian rhythm with energy homeostasis in humans. Diurnal rhythms of circulating FGF21 could be partly caused by the oscillation of free fatty acids. © 2011 American Association for Clinical Chemistry.
Persistent Identifierhttp://hdl.handle.net/10722/139448
ISSN
2015 Impact Factor: 7.457
2015 SCImago Journal Rankings: 2.472
ISI Accession Number ID
Funding AgencyGrant Number
Collaborative Research FundHKU3/09C
National 973 Project of China2011CB504001
Shanghai Municipality for Basic Research
National Basic Research Program of China2011CB504004
Funding Information:

K.S. Lam, Collaborative Research Fund (HKU3/09C); W. Jia, National 973 Project of China (2011CB504001) and Major Program of Shanghai Municipality for Basic Research; A. Xu, National Basic Research Program of China (2011CB504004). Expert Testimony: None declared.

References

 

DC FieldValueLanguage
dc.contributor.authorYu, Hen_HK
dc.contributor.authorXia, Fen_HK
dc.contributor.authorLam, KSLen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorBao, Yen_HK
dc.contributor.authorZhang, Jen_HK
dc.contributor.authorGu, Yen_HK
dc.contributor.authorZhou, Pen_HK
dc.contributor.authorLu, Jen_HK
dc.contributor.authorJia, Wen_HK
dc.contributor.authorXu, Aen_HK
dc.date.accessioned2011-09-23T05:50:03Z-
dc.date.available2011-09-23T05:50:03Z-
dc.date.issued2011en_HK
dc.identifier.citationClinical Chemistry, 2011, v. 57 n. 5, p. 691-700en_HK
dc.identifier.issn0009-9147en_HK
dc.identifier.urihttp://hdl.handle.net/10722/139448-
dc.description.abstractBACKGROUND: Fibroblast growth factor (FGF) 21 is an endocrine factor actively involved in glucose and lipid metabolism in rodents. However, little is known about its physiological function and regulation in humans. This study investigated the diurnal changes in circulating FGF21 concentrations and their association with other metabolic markers in both obese and lean individuals. METHODS: A total of 36 volunteers were assigned to 2 groups. One group received 3 standardized meals and another group was fasted for 24 h. Blood samples were drawn every 30 min throughout a 24-h period. Circulating FGF21 concentrations were measured with an in-house chemiluminescence immunoassay. The effects of fatty acids on hepatic production of FGF21 were determined by using real-time PCR. RESULTS: In both the fasting and standardized meals groups, circulating FGF21 began to rise at midnight, reaching a peak in the early morning and then declining to basal concentrations early in the afternoon. Baseline concentrations of circulating FGF21 were much higher in obese individuals than in lean individuals (P < 0.05). However, the magnitude of the nocturnal rise in circulating FGF21 was significantly blunted in obese individuals. The 24-h oscillatory pattern of circulating FGF21 resembled that of free fatty acids and cortisol, but was opposite to the patterns of insulin and glucose. Unsaturated fatty acids induced time-dependent expression of FGF21 mRNA in human hepatocytes. CONCLUSIONS: These findings support the role of FGF21 as an important metabolic regulator that integrates the circadian rhythm with energy homeostasis in humans. Diurnal rhythms of circulating FGF21 could be partly caused by the oscillation of free fatty acids. © 2011 American Association for Clinical Chemistry.en_HK
dc.languageengen_US
dc.publisherAmerican Association for Clinical Chemistry, Inc. The Journal's web site is located at http://www.clinchem.orgen_HK
dc.relation.ispartofClinical Chemistryen_HK
dc.subject.meshBlood Glucose - metabolism-
dc.subject.meshCircadian Rhythm-
dc.subject.meshFatty Acids - blood-
dc.subject.meshFibroblast Growth Factors - blood-
dc.subject.meshObesity - blood-
dc.titleCircadian rhythm of circulating fibroblast growth factor 21 is related to diurnal changes in fatty acids in humansen_HK
dc.typeArticleen_HK
dc.identifier.emailLam, KSL: ksllam@hku.hken_HK
dc.identifier.emailWang, Y: yuwanghk@hku.hken_HK
dc.identifier.emailXu, A: amxu@hkucc.hku.hken_HK
dc.identifier.authorityLam, KSL=rp00343en_HK
dc.identifier.authorityWang, Y=rp00239en_HK
dc.identifier.authorityXu, A=rp00485en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1373/clinchem.2010.155184en_HK
dc.identifier.pmid21325103-
dc.identifier.scopuseid_2-s2.0-79956113302en_HK
dc.identifier.hkuros193989en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79956113302&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume57en_HK
dc.identifier.issue5en_HK
dc.identifier.spage691en_HK
dc.identifier.epage700en_HK
dc.identifier.isiWOS:000289985100009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYu, H=12770509900en_HK
dc.identifier.scopusauthoridXia, F=53165129700en_HK
dc.identifier.scopusauthoridLam, KSL=8082870600en_HK
dc.identifier.scopusauthoridWang, Y=34973733700en_HK
dc.identifier.scopusauthoridBao, Y=36062711100en_HK
dc.identifier.scopusauthoridZhang, J=35504391800en_HK
dc.identifier.scopusauthoridGu, Y=53163822100en_HK
dc.identifier.scopusauthoridZhou, P=53165348900en_HK
dc.identifier.scopusauthoridLu, J=53164288700en_HK
dc.identifier.scopusauthoridJia, W=34768292900en_HK
dc.identifier.scopusauthoridXu, A=7202655409en_HK

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