File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The double-stranded RNA-binding protein PACT functions as a cellular activator of RIG-I to facilitate innate antiviral response

TitleThe double-stranded RNA-binding protein PACT functions as a cellular activator of RIG-I to facilitate innate antiviral response
Authors
Issue Date2011
PublisherCell Press. The Journal's web site is located at http://www.cellhostandmicrobe.com
Citation
Cell Host And Microbe, 2011, v. 9 n. 4, p. 299-309 How to Cite?
AbstractRIG-I, a virus sensor that triggers innate antiviral response, is a DExD/H box RNA helicase bearing structural similarity with Dicer, an RNase III-type nuclease that mediates RNA interference. Dicer requires double-stranded RNA-binding protein partners, such as PACT, for optimal activity. Here we show that PACT physically binds to the C-terminal repression domain of RIG-I and potently stimulates RIG-I-induced type I interferon production. PACT potentiates the activation of RIG-I by poly(I:C) of intermediate length. PACT also cooperates with RIG-I to sustain the activation of antiviral defense. Depletion of PACT substantially attenuates viral induction of interferons. The activation of RIG-I by PACT does not require double-stranded RNA-dependent protein kinase or Dicer, but is mediated by a direct interaction that leads to stimulation of its ATPase activity. Our findings reveal PACT as an important component in initiating and sustaining the RIG-I-dependent antiviral response. © 2011 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/138962
ISSN
2015 Impact Factor: 12.552
2015 SCImago Journal Rankings: 8.283
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Fund for the Control of Infectious DiseasesHSI/Lab-17
10091202
Hong Kong Research Grants CouncilHKU 7677/10M
HKU 7/CRF/09
Funding Information:

This work was supported by Hong Kong Research Fund for the Control of Infectious Diseases (HSI/Lab-17 and 10091202) and Hong Kong Research Grants Council (HKU 7677/10M and HKU 7/CRF/09). We thank Takashi Fujita, Dominique Garcin, Greg Hannon, Brian Lichty, and Jacques Perrault for gifts of reagent; and Abel Chun, Kuan-Teh Jeang, Allan Lau, Ting Lei, Vincent Tang, and Chi-Ming Wong for critical reading of the manuscript. K.-H.K., S.W.N.A., and D.-Y.J. designed the study. K.-H.K., P.-Y.L., M.-H.J.N., and K.-L.S. performed experiments. K.-H.K., S.W.N.A., and D.-Y.J. analyzed the data. K.-H.K. and D.-Y.J. wrote the paper.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorKok, KHen_HK
dc.contributor.authorLui, PYen_HK
dc.contributor.authorNg, MHJen_HK
dc.contributor.authorSiu, KLen_HK
dc.contributor.authorAu, SWNen_HK
dc.contributor.authorJin, DYen_HK
dc.date.accessioned2011-09-23T05:43:10Z-
dc.date.available2011-09-23T05:43:10Z-
dc.date.issued2011en_HK
dc.identifier.citationCell Host And Microbe, 2011, v. 9 n. 4, p. 299-309en_HK
dc.identifier.issn1931-3128en_HK
dc.identifier.urihttp://hdl.handle.net/10722/138962-
dc.description.abstractRIG-I, a virus sensor that triggers innate antiviral response, is a DExD/H box RNA helicase bearing structural similarity with Dicer, an RNase III-type nuclease that mediates RNA interference. Dicer requires double-stranded RNA-binding protein partners, such as PACT, for optimal activity. Here we show that PACT physically binds to the C-terminal repression domain of RIG-I and potently stimulates RIG-I-induced type I interferon production. PACT potentiates the activation of RIG-I by poly(I:C) of intermediate length. PACT also cooperates with RIG-I to sustain the activation of antiviral defense. Depletion of PACT substantially attenuates viral induction of interferons. The activation of RIG-I by PACT does not require double-stranded RNA-dependent protein kinase or Dicer, but is mediated by a direct interaction that leads to stimulation of its ATPase activity. Our findings reveal PACT as an important component in initiating and sustaining the RIG-I-dependent antiviral response. © 2011 Elsevier Inc.en_HK
dc.languageengen_US
dc.publisherCell Press. The Journal's web site is located at http://www.cellhostandmicrobe.comen_HK
dc.relation.ispartofCell Host and Microbeen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCercopithecus aethiopsen_HK
dc.subject.meshDEAD-box RNA Helicases - immunology - metabolismen_HK
dc.subject.meshHEK293 Cellsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunity, Innateen_HK
dc.subject.meshInterferon Type I - biosynthesisen_HK
dc.subject.meshMiceen_HK
dc.subject.meshPlasmids - geneticsen_HK
dc.subject.meshPoly I-C - immunologyen_HK
dc.subject.meshRNA Interferenceen_HK
dc.subject.meshRNA, Double-Stranded - metabolismen_HK
dc.subject.meshRNA, Small Interferingen_HK
dc.subject.meshRNA-Binding Proteins - metabolismen_HK
dc.subject.meshSignal Transduction - physiologyen_HK
dc.subject.meshVero Cellsen_HK
dc.subject.meshViruses - immunology - metabolismen_HK
dc.subject.mesheIF-2 Kinase - metabolism - physiologyen_HK
dc.titleThe double-stranded RNA-binding protein PACT functions as a cellular activator of RIG-I to facilitate innate antiviral responseen_HK
dc.typeArticleen_HK
dc.identifier.emailKok, KH:khkok@hku.hken_HK
dc.identifier.emailJin, DY:dyjin@hkucc.hku.hken_HK
dc.identifier.authorityKok, KH=rp01455en_HK
dc.identifier.authorityJin, DY=rp00452en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.chom.2011.03.007en_HK
dc.identifier.pmid21501829-
dc.identifier.scopuseid_2-s2.0-79955075657en_HK
dc.identifier.hkuros193194en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79955075657&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume9en_HK
dc.identifier.issue4en_HK
dc.identifier.spage299en_HK
dc.identifier.epage309en_HK
dc.identifier.eissn1934-6069-
dc.identifier.isiWOS:000290002500008-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectInhibition of RIG-I-dependent innate immunity by herpes simplex virus type 1 Us11 protein-
dc.relation.projectMolecular Pathology of Liver Cancer - a Multidisciplinary Study-
dc.identifier.scopusauthoridKok, KH=7006862631en_HK
dc.identifier.scopusauthoridLui, PY=53983090700en_HK
dc.identifier.scopusauthoridNg, MHJ=27067825500en_HK
dc.identifier.scopusauthoridSiu, KL=35327996300en_HK
dc.identifier.scopusauthoridAu, SWN=7005457819en_HK
dc.identifier.scopusauthoridJin, DY=7201973614en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats