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Conference Paper: 3Alpha,7beta-dihydroxy-5beta-cholan-24-oic acid N-(2-sulfoethyl)amide alters the microRNAs expression and promotes cell migration of human hepatocellular carcinoma cells

Title3Alpha,7beta-dihydroxy-5beta-cholan-24-oic acid N-(2-sulfoethyl)amide alters the microRNAs expression and promotes cell migration of human hepatocellular carcinoma cells
Authors
KeywordsMedical sciences
Oncology
Issue Date2011
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
The 2010 Excellence in Oncology Conference, Athens, Greece, 18-20 November 2010. In International Journal of Cancer, 2011, v. 128 suppl. 1, p. 39, abstract no. PP15a How to Cite?
AbstractINTRODUCTION: Hepatocellular carcinoma is one of the most malignant cancers with dismal outcomes and the therapeutic strategies remain poor and far from developed. 3alpha,7beta-Dihydroxy-5beta-cholan-24-oic Acid N-(2-Sulfoethyl) amide (Tauroursodeoxycholic Acid, TUDCA) is currently in frequent used for protect the hepatocyte in several kinds of liver disorders including liver cancer. MicroRNA-21 (miR-21) overexpression was reported correlated with the high invasive property of cancer cells. PURPOSE: To profile the microRNA expression alteration in hepatocarcinoma cells with treatment of TUDCA and observe the effect of TUDCA in the invasion and metastasis of hepatocellular carcinoma. Material: Human hepatocellular carcinoma cell line with high metastatic property MHCC97-L. METHODS: We developed a sensitive microRNA on-chip array to detect the miRNAs expression profiling in MHCC97-L cells with or without TUDCA exposure. A quantitative real-time PCR method was introduced to validate the on-chip result. The cell migration was detected by wound healing the invasion chamber assay. Inhibition of miR-21 was conducted by introducing an antisense oligonucleotide complementary to the miRNA. RESULTS: Three microRNAs, let-7f, miR-21 and miR-23a were up-regulated in MHCC97-L with 100 lM TUDCA exposure for 48 hr. The result of the qPCR validates the increase of miR-21 expression in MHCC97-L cells treated with TUDCA comparing with the control. Increased cell migration was observed in MHCC97-L cells with 100 lM TUDCA exposure. Addition of miR-21 inhibitor attenuates the effect of TUDCA on MHCC97-L cell migration.
DescriptionConference Theme: Cutting Edge Findings Into Clinical Practice
This FREE journal suppl. entitled: Supplement: Abstracts of the Excellence in Oncology 2010 Conference 18–20 November 2010, Hilton Hotel, Athens, Greece
Poster Presentations: no. PP15a
Persistent Identifierhttp://hdl.handle.net/10722/138266
ISSN
2014 Impact Factor: 5.085
2014 SCImago Journal Rankings: 2.175

 

DC FieldValueLanguage
dc.contributor.authorZhu, Men_US
dc.contributor.authorWang, Nen_US
dc.contributor.authorTsao, SWen_US
dc.contributor.authorTong, Yen_US
dc.contributor.authorFeng, Yen_US
dc.date.accessioned2011-08-26T14:43:58Z-
dc.date.available2011-08-26T14:43:58Z-
dc.date.issued2011en_US
dc.identifier.citationThe 2010 Excellence in Oncology Conference, Athens, Greece, 18-20 November 2010. In International Journal of Cancer, 2011, v. 128 suppl. 1, p. 39, abstract no. PP15aen_US
dc.identifier.issn0020-7136-
dc.identifier.urihttp://hdl.handle.net/10722/138266-
dc.descriptionConference Theme: Cutting Edge Findings Into Clinical Practice-
dc.descriptionThis FREE journal suppl. entitled: Supplement: Abstracts of the Excellence in Oncology 2010 Conference 18–20 November 2010, Hilton Hotel, Athens, Greece-
dc.descriptionPoster Presentations: no. PP15a-
dc.description.abstractINTRODUCTION: Hepatocellular carcinoma is one of the most malignant cancers with dismal outcomes and the therapeutic strategies remain poor and far from developed. 3alpha,7beta-Dihydroxy-5beta-cholan-24-oic Acid N-(2-Sulfoethyl) amide (Tauroursodeoxycholic Acid, TUDCA) is currently in frequent used for protect the hepatocyte in several kinds of liver disorders including liver cancer. MicroRNA-21 (miR-21) overexpression was reported correlated with the high invasive property of cancer cells. PURPOSE: To profile the microRNA expression alteration in hepatocarcinoma cells with treatment of TUDCA and observe the effect of TUDCA in the invasion and metastasis of hepatocellular carcinoma. Material: Human hepatocellular carcinoma cell line with high metastatic property MHCC97-L. METHODS: We developed a sensitive microRNA on-chip array to detect the miRNAs expression profiling in MHCC97-L cells with or without TUDCA exposure. A quantitative real-time PCR method was introduced to validate the on-chip result. The cell migration was detected by wound healing the invasion chamber assay. Inhibition of miR-21 was conducted by introducing an antisense oligonucleotide complementary to the miRNA. RESULTS: Three microRNAs, let-7f, miR-21 and miR-23a were up-regulated in MHCC97-L with 100 lM TUDCA exposure for 48 hr. The result of the qPCR validates the increase of miR-21 expression in MHCC97-L cells treated with TUDCA comparing with the control. Increased cell migration was observed in MHCC97-L cells with 100 lM TUDCA exposure. Addition of miR-21 inhibitor attenuates the effect of TUDCA on MHCC97-L cell migration.-
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home-
dc.relation.ispartofInternational Journal of Canceren_US
dc.rightsInternational Journal of Cancer . Copyright © John Wiley & Sons, Inc..-
dc.subjectMedical sciences-
dc.subjectOncology-
dc.title3Alpha,7beta-dihydroxy-5beta-cholan-24-oic acid N-(2-sulfoethyl)amide alters the microRNAs expression and promotes cell migration of human hepatocellular carcinoma cellsen_US
dc.typeConference_Paperen_US
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0020-7136&volume=128&issue=Supplement 1&spage=39, abstract no. PP15a&epage=&date=2011&atitle=3Alpha,7beta-dihydroxy-5beta-cholan-24-oic+acid+N-(2-sulfoethyl)amide+alters+the+microRNAs+expression+and+promotes+cell+migration+of+human+hepatocellular+carcinoma+cells-
dc.identifier.emailTsao, SW: gswtsao@hkucc.hku.hken_US
dc.identifier.emailTong, Y: tongyao@hku.hken_US
dc.identifier.emailFeng, Y: yfeng@hku.hken_US
dc.identifier.authorityTsao, SW=rp00399en_US
dc.identifier.authorityTong, Y=rp00509en_US
dc.identifier.authorityFeng, Y=rp00466en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/ijc.25877-
dc.identifier.hkuros191236en_US
dc.identifier.volume128-
dc.identifier.issuesuppl. 1-
dc.identifier.spage39, abstract no. PP15a-
dc.identifier.epage39, abstract no. PP15a-
dc.description.otherExcellence in Oncology: Cutting Edge Findings Into Clinical Practice, Athens, Greece, 18-20 November 2010. In International Journal of Cancer, 2011, v. 128 suppl. 1, p. 39, abstract no. PP15a-

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