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Conference Paper: Tumor necrosis factor-α up-regulates the microRNA-21 expression and promotes cell migration in human hepatocellular carcinoma cells MHCC97-L

TitleTumor necrosis factor-α up-regulates the microRNA-21 expression and promotes cell migration in human hepatocellular carcinoma cells MHCC97-L
Authors
KeywordsMedical sciences
Oncology
Issue Date2011
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
The 2010 Excellence in Oncology Conference, Athens, Greece, 18-20 November 2010. In International Journal of Cancer, 2011, v. 128 suppl. 1, p. 38-39, abstract no. PP14 How to Cite?
AbstractINTRODUCTION: Inflammation of the liver is often observed in the progression of hepatocellular carcinoma. The tumor necrosis factor-a (TNF-a) is one of the major cytokine that may be involved in the inflammation process. microRNA-21 has been reported to play a role in the invasion and metastasis in different kinds of human cancers. PURPOSE: The aim of this study is to involvement of microRNA-21 in the inflammation induced human hepatocellular carcinoma invasion and metastasis in vitro. Material: The human hepatocellular carcinoma cell line with high metastatic property MHCC97-L was used. METHODS: The microRNA expression profile of MHCC97-L upon sustained 10 ng/mL TNF-a exposure was obtained by on-chip microRNA array. The expression of altered microRNAs was validated by quantitative real-time PCR. The cell migration of MHCC97-L cells was measured by wound healing and invasion chamber assay. Inhibition of microRNA-21 was conducted by introducing an antisense oligonucleotide specifically complementary to microRNA-21 into cells. RESULTS: The expression profile of microRNAs in MHCC97-L cells is altered upon 48hr exposure to TNF-a. Up-regulations of microRNA-21, microRNA-146a, microRNA-200b, microRNA-25 and microRNA-29a are observed. The increased expression of microRNA-21 (metastatic related) and microRNA-146a (inflammation related) is validated by qPCR analysis. Increase of cell migration is confirmed in both wound healing assay and invasion chamber assay. Transfection with microRNA-21 antisense oligonucleotide attenuates the migration activity of MHCC97-L induced by TNF-a. CONCLUSION: Exposure of TNF-a could promote the metastatic property of the hepatocellular carcinoma cells MHCC97-L and the over expression of microRNA-21 may play a role.
DescriptionConference Theme: Cutting Edge Findings Into Clinical Practice
This FREE journal suppl. entitled: Supplement: Abstracts of the Excellence in Oncology 2010 Conference 18–20 November 2010, Hilton Hotel, Athens, Greece
Poster Presentations: no. PP14
Persistent Identifierhttp://hdl.handle.net/10722/138265
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.657

 

DC FieldValueLanguage
dc.contributor.authorWang, Nen_US
dc.contributor.authorZhu, Men_US
dc.contributor.authorTsao, SWen_US
dc.contributor.authorTong, Yen_US
dc.contributor.authorFeng, Yen_US
dc.date.accessioned2011-08-26T14:43:58Z-
dc.date.available2011-08-26T14:43:58Z-
dc.date.issued2011en_US
dc.identifier.citationThe 2010 Excellence in Oncology Conference, Athens, Greece, 18-20 November 2010. In International Journal of Cancer, 2011, v. 128 suppl. 1, p. 38-39, abstract no. PP14en_US
dc.identifier.issn0020-7136-
dc.identifier.urihttp://hdl.handle.net/10722/138265-
dc.descriptionConference Theme: Cutting Edge Findings Into Clinical Practice-
dc.descriptionThis FREE journal suppl. entitled: Supplement: Abstracts of the Excellence in Oncology 2010 Conference 18–20 November 2010, Hilton Hotel, Athens, Greece-
dc.descriptionPoster Presentations: no. PP14-
dc.description.abstractINTRODUCTION: Inflammation of the liver is often observed in the progression of hepatocellular carcinoma. The tumor necrosis factor-a (TNF-a) is one of the major cytokine that may be involved in the inflammation process. microRNA-21 has been reported to play a role in the invasion and metastasis in different kinds of human cancers. PURPOSE: The aim of this study is to involvement of microRNA-21 in the inflammation induced human hepatocellular carcinoma invasion and metastasis in vitro. Material: The human hepatocellular carcinoma cell line with high metastatic property MHCC97-L was used. METHODS: The microRNA expression profile of MHCC97-L upon sustained 10 ng/mL TNF-a exposure was obtained by on-chip microRNA array. The expression of altered microRNAs was validated by quantitative real-time PCR. The cell migration of MHCC97-L cells was measured by wound healing and invasion chamber assay. Inhibition of microRNA-21 was conducted by introducing an antisense oligonucleotide specifically complementary to microRNA-21 into cells. RESULTS: The expression profile of microRNAs in MHCC97-L cells is altered upon 48hr exposure to TNF-a. Up-regulations of microRNA-21, microRNA-146a, microRNA-200b, microRNA-25 and microRNA-29a are observed. The increased expression of microRNA-21 (metastatic related) and microRNA-146a (inflammation related) is validated by qPCR analysis. Increase of cell migration is confirmed in both wound healing assay and invasion chamber assay. Transfection with microRNA-21 antisense oligonucleotide attenuates the migration activity of MHCC97-L induced by TNF-a. CONCLUSION: Exposure of TNF-a could promote the metastatic property of the hepatocellular carcinoma cells MHCC97-L and the over expression of microRNA-21 may play a role.-
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home-
dc.relation.ispartofInternational Journal of Canceren_US
dc.rightsInternational Journal of Cancer. Copyright © John Wiley & Sons, Inc..-
dc.subjectMedical sciences-
dc.subjectOncology-
dc.titleTumor necrosis factor-α up-regulates the microRNA-21 expression and promotes cell migration in human hepatocellular carcinoma cells MHCC97-Len_US
dc.typeConference_Paperen_US
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0020-7136&volume=128&issue=Supplement 1&spage=38&epage=39, abstract no. PP14&date=2011&atitle=Tumor+necrosis+factor-α+up-regulates+the+microRNA-21+expression+and+promotes+cell+migration+in+human+hepatocellular+carcinoma+cells+MHCC97-L-
dc.identifier.emailTsao, SW: gswtsao@hkucc.hku.hken_US
dc.identifier.emailTong, Y: tongyao@hku.hken_US
dc.identifier.emailFeng, Y: yfeng@hku.hken_US
dc.identifier.authorityTsao, SW=rp00399en_US
dc.identifier.authorityTong, Y=rp00509en_US
dc.identifier.authorityFeng, Y=rp00466en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/ijc.25877-
dc.identifier.hkuros191235en_US
dc.identifier.volume128-
dc.identifier.issuesuppl. 1-
dc.identifier.spage38, abstract no. PP14-
dc.identifier.epage39-
dc.description.otherExcellence in Oncology: Cutting Edge Findings Into Clinical Practice, Athens, Greece, 18-20 November 2010. In International Journal of Cancer, 2011, v. 128 suppl. 1, p. 38-39, abstract no. PP14-

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