Article: Chemical and biological analysis of active free and conjugated bile acids in animal bile using HPLC-ELSD and MTT methods

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TitleChemical and biological analysis of active free and conjugated bile acids in animal bile using HPLC-ELSD and MTT methods
AuthorsWang, N1
Feng, Y1
Xie, TN3
Su, W3
Zhu, M1
Chow, O1
Zhang, Y1
WanMing, NG2
Leung, CH2
Tong, Y1
KeywordsAnimal bile
Bile acids
Cytotoxicity
Hepatocellular carcinoma
High performance liquid chromatography-evaporative light scattering detector system
Issue Date2011
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com
CitationExperimental And Therapeutic Medicine, 2011, v. 2 n. 1, p. 125-130 [How to Cite?]
DOI: http://dx.doi.org/10.3892/etm.2010.178
AbstractThe aim of the present study was to determine the chemical composition and in vitro cytotoxic activity of seven bile samples and bile acids using the high-performance liquid chromatography (HPLC)-evaporative light scattering detector (ELSD) method. Free and conjugated bile acid standards were used to identify and quantify the chemical components of the seven animal bile samples. The MTT assay was used to determine the cytotoxic effect of the animal bile samples and the free and conjugated bile acids on hepatocellular carcinoma MHCC97-Lcells. Chemical analysis revealed that the bile samples from the different animals shared little similarity in terms of their composition. A cell viability assay revealed that cattle bile, as well as its major components, DCA, CDCA and TCDCA, exhibited a marked cytotoxic effect on the hepatocellular carcinoma MHCC97-L cells. The bear bile samples that originated from the Asian black bear and the American black bear contained a unique component, TUDCA, which distinguished them from the other animal bile, though their inhibitory action on MHCC97-Lcells was not markedly distinct. The present study reveals that cattle bile may be a potential alternative to bear bile for hepatocarcinoma therapy.
ISSN1792-0981
2011 Impact Factor: 0.206
DOIhttp://dx.doi.org/10.3892/etm.2010.178
ISI Accession Number IDWOS:000286365000019
Funding AgencyGrant Number
Research Council of the University of Hong Kong200811159197
200907176140
Research Grant Council (RGC) of Hong Kong SAR, China764708M
Pong Ding Yueng Endowment Fund for Education and Research in Chinese-Western Medicine20005274
Hong Kong Government20740314
Funding Information:

This study was supported by grants from the Research Council of the University of Hong Kong (project codes 200811159197 and 200907176140), the Research Grant Council (RGC) of Hong Kong SAR, China (project code 764708M), Pong Ding Yueng Endowment Fund for Education and Research in Chinese-Western Medicine (project code: 20005274) and Hong Kong Government-Matching Grant Scheme (4th phase, project code: 20740314). The cell line MHCC97-L was a kind gift from the Liver Cancer Institute of Fudan University, Shanghai, China. The authors are grateful for the support of Professors Sai-Wah Tsao, Kwan Man, Yung-Chi Cheng, Chi-Ming Che and Allan S.Y. Lau. The authors would like to express thanks to Dr Ka-Yu Siu, Ms. Cindy Lee, Mr. Keith Wong and Mr. Freddy Tsang for their technical support.

ReferencesReferences in Scopus
GrantsThe role of autophagy and mitochondrial apoptosis in berberine induced hepatocellular caricinoma cell death and its underlying mechanism
The effects of berberine and its mechanism on angiogenesis in hepatocellular carcinoma in vitro and in vivo systems
DC Field
Value
dc.contributor.authorWang, N
dc.contributor.authorFeng, Y
dc.contributor.authorXie, TN
dc.contributor.authorSu, W
dc.contributor.authorZhu, M
dc.contributor.authorChow, O
dc.contributor.authorZhang, Y
dc.contributor.authorWanMing, NG
dc.contributor.authorLeung, CH
dc.contributor.authorTong, Y
dc.date.accessioned2011-08-26T14:41:21Z
dc.date.available2011-08-26T14:41:21Z
dc.date.issued2011
dc.description.abstractThe aim of the present study was to determine the chemical composition and in vitro cytotoxic activity of seven bile samples and bile acids using the high-performance liquid chromatography (HPLC)-evaporative light scattering detector (ELSD) method. Free and conjugated bile acid standards were used to identify and quantify the chemical components of the seven animal bile samples. The MTT assay was used to determine the cytotoxic effect of the animal bile samples and the free and conjugated bile acids on hepatocellular carcinoma MHCC97-Lcells. Chemical analysis revealed that the bile samples from the different animals shared little similarity in terms of their composition. A cell viability assay revealed that cattle bile, as well as its major components, DCA, CDCA and TCDCA, exhibited a marked cytotoxic effect on the hepatocellular carcinoma MHCC97-L cells. The bear bile samples that originated from the Asian black bear and the American black bear contained a unique component, TUDCA, which distinguished them from the other animal bile, though their inhibitory action on MHCC97-Lcells was not markedly distinct. The present study reveals that cattle bile may be a potential alternative to bear bile for hepatocarcinoma therapy.
dc.description.grantThe role of autophagy and mitochondrial apoptosis in berberine induced hepatocellular caricinoma cell death and its underlying mechanism
dc.description.grantThe effects of berberine and its mechanism on angiogenesis in hepatocellular carcinoma in vitro and in vivo systems
dc.description.grantcode101411
dc.description.grantcode99722
dc.description.naturelink_to_OA_fulltext
dc.identifier.citationExperimental And Therapeutic Medicine, 2011, v. 2 n. 1, p. 125-130 [How to Cite?]
DOI: http://dx.doi.org/10.3892/etm.2010.178
dc.identifier.doihttp://dx.doi.org/10.3892/etm.2010.178
dc.identifier.epage130
dc.identifier.hkuros191221
dc.identifier.isiWOS:000286365000019
Funding AgencyGrant Number
Research Council of the University of Hong Kong200811159197
200907176140
Research Grant Council (RGC) of Hong Kong SAR, China764708M
Pong Ding Yueng Endowment Fund for Education and Research in Chinese-Western Medicine20005274
Hong Kong Government20740314
Funding Information:

This study was supported by grants from the Research Council of the University of Hong Kong (project codes 200811159197 and 200907176140), the Research Grant Council (RGC) of Hong Kong SAR, China (project code 764708M), Pong Ding Yueng Endowment Fund for Education and Research in Chinese-Western Medicine (project code: 20005274) and Hong Kong Government-Matching Grant Scheme (4th phase, project code: 20740314). The cell line MHCC97-L was a kind gift from the Liver Cancer Institute of Fudan University, Shanghai, China. The authors are grateful for the support of Professors Sai-Wah Tsao, Kwan Man, Yung-Chi Cheng, Chi-Ming Che and Allan S.Y. Lau. The authors would like to express thanks to Dr Ka-Yu Siu, Ms. Cindy Lee, Mr. Keith Wong and Mr. Freddy Tsang for their technical support.

dc.identifier.issn1792-0981
2011 Impact Factor: 0.206
dc.identifier.issue1
dc.identifier.openurl
dc.identifier.scopuseid_2-s2.0-78751637141
dc.identifier.spage125
dc.identifier.urihttp://hdl.handle.net/10722/138138
dc.identifier.volume2
dc.languageeng
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com
dc.publisher.placeGreece
dc.relation.ispartofExperimental and Therapeutic Medicine
dc.relation.referencesReferences in Scopus
dc.subjectAnimal bile
dc.subjectBile acids
dc.subjectCytotoxicity
dc.subjectHepatocellular carcinoma
dc.subjectHigh performance liquid chromatography-evaporative light scattering detector system
dc.titleChemical and biological analysis of active free and conjugated bile acids in animal bile using HPLC-ELSD and MTT methods
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Institute of Molecular Technology for Drug Discovery and Synthesis, Hong Kong
  3. Sun Yat-Sen University