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- Publisher Website: 10.3892/etm.2010.178
- Scopus: eid_2-s2.0-78751637141
- PMID: 22977479
- WOS: WOS:000286365000019
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Article: Chemical and biological analysis of active free and conjugated bile acids in animal bile using HPLC-ELSD and MTT methods
Title | Chemical and biological analysis of active free and conjugated bile acids in animal bile using HPLC-ELSD and MTT methods | ||||||||||
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Authors | |||||||||||
Keywords | Animal bile Bile acids Cytotoxicity Hepatocellular carcinoma High performance liquid chromatography-evaporative light scattering detector system | ||||||||||
Issue Date | 2011 | ||||||||||
Publisher | Spandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com | ||||||||||
Citation | Experimental and Therapeutic Medicine, 2011, v. 2 n. 1, p. 125-130 How to Cite? | ||||||||||
Abstract | The aim of the present study was to determine the chemical composition and in vitro cytotoxic activity of seven bile samples and bile acids using the high-performance liquid chromatography (HPLC)-evaporative light scattering detector (ELSD) method. Free and conjugated bile acid standards were used to identify and quantify the chemical components of the seven animal bile samples. The MTT assay was used to determine the cytotoxic effect of the animal bile samples and the free and conjugated bile acids on hepatocellular carcinoma MHCC97-Lcells. Chemical analysis revealed that the bile samples from the different animals shared little similarity in terms of their composition. A cell viability assay revealed that cattle bile, as well as its major components, DCA, CDCA and TCDCA, exhibited a marked cytotoxic effect on the hepatocellular carcinoma MHCC97-L cells. The bear bile samples that originated from the Asian black bear and the American black bear contained a unique component, TUDCA, which distinguished them from the other animal bile, though their inhibitory action on MHCC97-Lcells was not markedly distinct. The present study reveals that cattle bile may be a potential alternative to bear bile for hepatocarcinoma therapy. | ||||||||||
Persistent Identifier | http://hdl.handle.net/10722/138138 | ||||||||||
ISSN | 2023 Impact Factor: 2.4 2019 SCImago Journal Rankings: 0.508 | ||||||||||
PubMed Central ID | |||||||||||
ISI Accession Number ID |
Funding Information: This study was supported by grants from the Research Council of the University of Hong Kong (project codes 200811159197 and 200907176140), the Research Grant Council (RGC) of Hong Kong SAR, China (project code 764708M), Pong Ding Yueng Endowment Fund for Education and Research in Chinese-Western Medicine (project code: 20005274) and Hong Kong Government-Matching Grant Scheme (4th phase, project code: 20740314). The cell line MHCC97-L was a kind gift from the Liver Cancer Institute of Fudan University, Shanghai, China. The authors are grateful for the support of Professors Sai-Wah Tsao, Kwan Man, Yung-Chi Cheng, Chi-Ming Che and Allan S.Y. Lau. The authors would like to express thanks to Dr Ka-Yu Siu, Ms. Cindy Lee, Mr. Keith Wong and Mr. Freddy Tsang for their technical support. | ||||||||||
References | |||||||||||
Grants |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wang, N | en_HK |
dc.contributor.author | Feng, Y | en_HK |
dc.contributor.author | Xie, TN | en_HK |
dc.contributor.author | Su, W | en_HK |
dc.contributor.author | Zhu, M | en_HK |
dc.contributor.author | Chow, O | en_HK |
dc.contributor.author | Zhang, Y | en_HK |
dc.contributor.author | Ng, KM | en_HK |
dc.contributor.author | Leung, CH | en_HK |
dc.contributor.author | Tong, Y | en_HK |
dc.date.accessioned | 2011-08-26T14:41:21Z | - |
dc.date.available | 2011-08-26T14:41:21Z | - |
dc.date.issued | 2011 | en_HK |
dc.identifier.citation | Experimental and Therapeutic Medicine, 2011, v. 2 n. 1, p. 125-130 | en_HK |
dc.identifier.issn | 1792-0981 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/138138 | - |
dc.description.abstract | The aim of the present study was to determine the chemical composition and in vitro cytotoxic activity of seven bile samples and bile acids using the high-performance liquid chromatography (HPLC)-evaporative light scattering detector (ELSD) method. Free and conjugated bile acid standards were used to identify and quantify the chemical components of the seven animal bile samples. The MTT assay was used to determine the cytotoxic effect of the animal bile samples and the free and conjugated bile acids on hepatocellular carcinoma MHCC97-Lcells. Chemical analysis revealed that the bile samples from the different animals shared little similarity in terms of their composition. A cell viability assay revealed that cattle bile, as well as its major components, DCA, CDCA and TCDCA, exhibited a marked cytotoxic effect on the hepatocellular carcinoma MHCC97-L cells. The bear bile samples that originated from the Asian black bear and the American black bear contained a unique component, TUDCA, which distinguished them from the other animal bile, though their inhibitory action on MHCC97-Lcells was not markedly distinct. The present study reveals that cattle bile may be a potential alternative to bear bile for hepatocarcinoma therapy. | en_HK |
dc.language | eng | en_US |
dc.publisher | Spandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com | en_HK |
dc.relation.ispartof | Experimental and Therapeutic Medicine | en_HK |
dc.subject | Animal bile | en_HK |
dc.subject | Bile acids | en_HK |
dc.subject | Cytotoxicity | en_HK |
dc.subject | Hepatocellular carcinoma | en_HK |
dc.subject | High performance liquid chromatography-evaporative light scattering detector system | en_HK |
dc.title | Chemical and biological analysis of active free and conjugated bile acids in animal bile using HPLC-ELSD and MTT methods | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Feng, Y: yfeng@hku.hk | en_HK |
dc.identifier.email | Zhang, Y: ybzhang@hku.hk | en_HK |
dc.identifier.email | Ng, KM: kwanmng@hku.hk | en_HK |
dc.identifier.email | Leung, CH: duncanl@hkucc.hku.hk | en_HK |
dc.identifier.email | Tong, Y: tongyao@hku.hk | en_HK |
dc.identifier.authority | Feng, Y=rp00466 | en_HK |
dc.identifier.authority | Zhang, Y=rp01410 | en_HK |
dc.identifier.authority | Ng, KM=rp00766 | en_HK |
dc.identifier.authority | Leung, CH=rp00730 | en_HK |
dc.identifier.authority | Tong, Y=rp00509 | en_HK |
dc.description.nature | link_to_OA_fulltext | - |
dc.identifier.doi | 10.3892/etm.2010.178 | en_HK |
dc.identifier.pmid | 22977479 | - |
dc.identifier.pmcid | PMC3440638 | - |
dc.identifier.scopus | eid_2-s2.0-78751637141 | en_HK |
dc.identifier.hkuros | 191221 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-78751637141&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 2 | en_HK |
dc.identifier.issue | 1 | en_HK |
dc.identifier.spage | 125 | en_HK |
dc.identifier.epage | 130 | en_HK |
dc.identifier.isi | WOS:000286365000019 | - |
dc.publisher.place | Greece | en_HK |
dc.relation.project | The effects of berberine and its mechanism on angiogenesis in hepatocellular carcinoma in vitro and in vivo systems | - |
dc.relation.project | The role of autophagy and mitochondrial apoptosis in berberine induced hepatocellular caricinoma cell death and its underlying mechanism | - |
dc.identifier.scopusauthorid | Wang, N=35072317700 | en_HK |
dc.identifier.scopusauthorid | Feng, Y=24467969600 | en_HK |
dc.identifier.scopusauthorid | Xie, TN=36984406000 | en_HK |
dc.identifier.scopusauthorid | Su, W=7402010268 | en_HK |
dc.identifier.scopusauthorid | Zhu, M=36706949300 | en_HK |
dc.identifier.scopusauthorid | Chow, O=36983856000 | en_HK |
dc.identifier.scopusauthorid | Zhang, Y=23483121900 | en_HK |
dc.identifier.scopusauthorid | Ng, KM=26026091100 | en_HK |
dc.identifier.scopusauthorid | Leung, CH=7402612570 | en_HK |
dc.identifier.scopusauthorid | Tong, Y=9045384000 | en_HK |
dc.identifier.issnl | 1792-0981 | - |