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Article: Chemical and biological analysis of active free and conjugated bile acids in animal bile using HPLC-ELSD and MTT methods
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TitleChemical and biological analysis of active free and conjugated bile acids in animal bile using HPLC-ELSD and MTT methods
 
AuthorsWang, N1
Feng, Y1
Xie, TN3
Su, W3
Zhu, M1
Chow, O1
Zhang, Y1
WanMing, NG2
Leung, CH2
Tong, Y1
 
KeywordsAnimal bile
Bile acids
Cytotoxicity
Hepatocellular carcinoma
High performance liquid chromatography-evaporative light scattering detector system
 
Issue Date2011
 
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com
 
CitationExperimental And Therapeutic Medicine, 2011, v. 2 n. 1, p. 125-130 [How to Cite?]
DOI: http://dx.doi.org/10.3892/etm.2010.178
 
AbstractThe aim of the present study was to determine the chemical composition and in vitro cytotoxic activity of seven bile samples and bile acids using the high-performance liquid chromatography (HPLC)-evaporative light scattering detector (ELSD) method. Free and conjugated bile acid standards were used to identify and quantify the chemical components of the seven animal bile samples. The MTT assay was used to determine the cytotoxic effect of the animal bile samples and the free and conjugated bile acids on hepatocellular carcinoma MHCC97-Lcells. Chemical analysis revealed that the bile samples from the different animals shared little similarity in terms of their composition. A cell viability assay revealed that cattle bile, as well as its major components, DCA, CDCA and TCDCA, exhibited a marked cytotoxic effect on the hepatocellular carcinoma MHCC97-L cells. The bear bile samples that originated from the Asian black bear and the American black bear contained a unique component, TUDCA, which distinguished them from the other animal bile, though their inhibitory action on MHCC97-Lcells was not markedly distinct. The present study reveals that cattle bile may be a potential alternative to bear bile for hepatocarcinoma therapy.
 
ISSN1792-0981
2012 Impact Factor: 0.344
 
DOIhttp://dx.doi.org/10.3892/etm.2010.178
 
PubMed Central IDPMC3440638
 
ISI Accession Number IDWOS:000286365000019
Funding AgencyGrant Number
Research Council of the University of Hong Kong200811159197
200907176140
Research Grant Council (RGC) of Hong Kong SAR, China764708M
Pong Ding Yueng Endowment Fund for Education and Research in Chinese-Western Medicine20005274
Hong Kong Government20740314
Funding Information:

This study was supported by grants from the Research Council of the University of Hong Kong (project codes 200811159197 and 200907176140), the Research Grant Council (RGC) of Hong Kong SAR, China (project code 764708M), Pong Ding Yueng Endowment Fund for Education and Research in Chinese-Western Medicine (project code: 20005274) and Hong Kong Government-Matching Grant Scheme (4th phase, project code: 20740314). The cell line MHCC97-L was a kind gift from the Liver Cancer Institute of Fudan University, Shanghai, China. The authors are grateful for the support of Professors Sai-Wah Tsao, Kwan Man, Yung-Chi Cheng, Chi-Ming Che and Allan S.Y. Lau. The authors would like to express thanks to Dr Ka-Yu Siu, Ms. Cindy Lee, Mr. Keith Wong and Mr. Freddy Tsang for their technical support.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorWang, N
 
dc.contributor.authorFeng, Y
 
dc.contributor.authorXie, TN
 
dc.contributor.authorSu, W
 
dc.contributor.authorZhu, M
 
dc.contributor.authorChow, O
 
dc.contributor.authorZhang, Y
 
dc.contributor.authorWanMing, NG
 
dc.contributor.authorLeung, CH
 
dc.contributor.authorTong, Y
 
dc.date.accessioned2011-08-26T14:41:21Z
 
dc.date.available2011-08-26T14:41:21Z
 
dc.date.issued2011
 
dc.description.abstractThe aim of the present study was to determine the chemical composition and in vitro cytotoxic activity of seven bile samples and bile acids using the high-performance liquid chromatography (HPLC)-evaporative light scattering detector (ELSD) method. Free and conjugated bile acid standards were used to identify and quantify the chemical components of the seven animal bile samples. The MTT assay was used to determine the cytotoxic effect of the animal bile samples and the free and conjugated bile acids on hepatocellular carcinoma MHCC97-Lcells. Chemical analysis revealed that the bile samples from the different animals shared little similarity in terms of their composition. A cell viability assay revealed that cattle bile, as well as its major components, DCA, CDCA and TCDCA, exhibited a marked cytotoxic effect on the hepatocellular carcinoma MHCC97-L cells. The bear bile samples that originated from the Asian black bear and the American black bear contained a unique component, TUDCA, which distinguished them from the other animal bile, though their inhibitory action on MHCC97-Lcells was not markedly distinct. The present study reveals that cattle bile may be a potential alternative to bear bile for hepatocarcinoma therapy.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationExperimental And Therapeutic Medicine, 2011, v. 2 n. 1, p. 125-130 [How to Cite?]
DOI: http://dx.doi.org/10.3892/etm.2010.178
 
dc.identifier.doihttp://dx.doi.org/10.3892/etm.2010.178
 
dc.identifier.epage130
 
dc.identifier.hkuros191221
 
dc.identifier.isiWOS:000286365000019
Funding AgencyGrant Number
Research Council of the University of Hong Kong200811159197
200907176140
Research Grant Council (RGC) of Hong Kong SAR, China764708M
Pong Ding Yueng Endowment Fund for Education and Research in Chinese-Western Medicine20005274
Hong Kong Government20740314
Funding Information:

This study was supported by grants from the Research Council of the University of Hong Kong (project codes 200811159197 and 200907176140), the Research Grant Council (RGC) of Hong Kong SAR, China (project code 764708M), Pong Ding Yueng Endowment Fund for Education and Research in Chinese-Western Medicine (project code: 20005274) and Hong Kong Government-Matching Grant Scheme (4th phase, project code: 20740314). The cell line MHCC97-L was a kind gift from the Liver Cancer Institute of Fudan University, Shanghai, China. The authors are grateful for the support of Professors Sai-Wah Tsao, Kwan Man, Yung-Chi Cheng, Chi-Ming Che and Allan S.Y. Lau. The authors would like to express thanks to Dr Ka-Yu Siu, Ms. Cindy Lee, Mr. Keith Wong and Mr. Freddy Tsang for their technical support.

 
dc.identifier.issn1792-0981
2012 Impact Factor: 0.344
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.pmcidPMC3440638
 
dc.identifier.scopuseid_2-s2.0-78751637141
 
dc.identifier.spage125
 
dc.identifier.urihttp://hdl.handle.net/10722/138138
 
dc.identifier.volume2
 
dc.languageeng
 
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com
 
dc.publisher.placeGreece
 
dc.relation.ispartofExperimental and Therapeutic Medicine
 
dc.relation.referencesReferences in Scopus
 
dc.subjectAnimal bile
 
dc.subjectBile acids
 
dc.subjectCytotoxicity
 
dc.subjectHepatocellular carcinoma
 
dc.subjectHigh performance liquid chromatography-evaporative light scattering detector system
 
dc.titleChemical and biological analysis of active free and conjugated bile acids in animal bile using HPLC-ELSD and MTT methods
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. Institute of Molecular Technology for Drug Discovery and Synthesis, Hong Kong
  3. Sun Yat-Sen University