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Article: Chemical and biological analysis of active free and conjugated bile acids in animal bile using HPLC-ELSD and MTT methods

TitleChemical and biological analysis of active free and conjugated bile acids in animal bile using HPLC-ELSD and MTT methods
Authors
KeywordsAnimal bile
Bile acids
Cytotoxicity
Hepatocellular carcinoma
High performance liquid chromatography-evaporative light scattering detector system
Issue Date2011
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com
Citation
Experimental And Therapeutic Medicine, 2011, v. 2 n. 1, p. 125-130 How to Cite?
Abstract
The aim of the present study was to determine the chemical composition and in vitro cytotoxic activity of seven bile samples and bile acids using the high-performance liquid chromatography (HPLC)-evaporative light scattering detector (ELSD) method. Free and conjugated bile acid standards were used to identify and quantify the chemical components of the seven animal bile samples. The MTT assay was used to determine the cytotoxic effect of the animal bile samples and the free and conjugated bile acids on hepatocellular carcinoma MHCC97-Lcells. Chemical analysis revealed that the bile samples from the different animals shared little similarity in terms of their composition. A cell viability assay revealed that cattle bile, as well as its major components, DCA, CDCA and TCDCA, exhibited a marked cytotoxic effect on the hepatocellular carcinoma MHCC97-L cells. The bear bile samples that originated from the Asian black bear and the American black bear contained a unique component, TUDCA, which distinguished them from the other animal bile, though their inhibitory action on MHCC97-Lcells was not markedly distinct. The present study reveals that cattle bile may be a potential alternative to bear bile for hepatocarcinoma therapy.
Persistent Identifierhttp://hdl.handle.net/10722/138138
ISSN
2013 Impact Factor: 0.941
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Research Council of the University of Hong Kong200811159197
200907176140
Research Grant Council (RGC) of Hong Kong SAR, China764708M
Pong Ding Yueng Endowment Fund for Education and Research in Chinese-Western Medicine20005274
Hong Kong Government20740314
Funding Information:

This study was supported by grants from the Research Council of the University of Hong Kong (project codes 200811159197 and 200907176140), the Research Grant Council (RGC) of Hong Kong SAR, China (project code 764708M), Pong Ding Yueng Endowment Fund for Education and Research in Chinese-Western Medicine (project code: 20005274) and Hong Kong Government-Matching Grant Scheme (4th phase, project code: 20740314). The cell line MHCC97-L was a kind gift from the Liver Cancer Institute of Fudan University, Shanghai, China. The authors are grateful for the support of Professors Sai-Wah Tsao, Kwan Man, Yung-Chi Cheng, Chi-Ming Che and Allan S.Y. Lau. The authors would like to express thanks to Dr Ka-Yu Siu, Ms. Cindy Lee, Mr. Keith Wong and Mr. Freddy Tsang for their technical support.

References

 

Author Affiliations
  1. The University of Hong Kong
  2. Institute of Molecular Technology for Drug Discovery and Synthesis, Hong Kong
  3. Sun Yat-Sen University
DC FieldValueLanguage
dc.contributor.authorWang, Nen_HK
dc.contributor.authorFeng, Yen_HK
dc.contributor.authorXie, TNen_HK
dc.contributor.authorSu, Wen_HK
dc.contributor.authorZhu, Men_HK
dc.contributor.authorChow, Oen_HK
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorWanMing, NGen_HK
dc.contributor.authorLeung, CHen_HK
dc.contributor.authorTong, Yen_HK
dc.date.accessioned2011-08-26T14:41:21Z-
dc.date.available2011-08-26T14:41:21Z-
dc.date.issued2011en_HK
dc.identifier.citationExperimental And Therapeutic Medicine, 2011, v. 2 n. 1, p. 125-130en_HK
dc.identifier.issn1792-0981en_HK
dc.identifier.urihttp://hdl.handle.net/10722/138138-
dc.description.abstractThe aim of the present study was to determine the chemical composition and in vitro cytotoxic activity of seven bile samples and bile acids using the high-performance liquid chromatography (HPLC)-evaporative light scattering detector (ELSD) method. Free and conjugated bile acid standards were used to identify and quantify the chemical components of the seven animal bile samples. The MTT assay was used to determine the cytotoxic effect of the animal bile samples and the free and conjugated bile acids on hepatocellular carcinoma MHCC97-Lcells. Chemical analysis revealed that the bile samples from the different animals shared little similarity in terms of their composition. A cell viability assay revealed that cattle bile, as well as its major components, DCA, CDCA and TCDCA, exhibited a marked cytotoxic effect on the hepatocellular carcinoma MHCC97-L cells. The bear bile samples that originated from the Asian black bear and the American black bear contained a unique component, TUDCA, which distinguished them from the other animal bile, though their inhibitory action on MHCC97-Lcells was not markedly distinct. The present study reveals that cattle bile may be a potential alternative to bear bile for hepatocarcinoma therapy.en_HK
dc.languageengen_US
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.comen_HK
dc.relation.ispartofExperimental and Therapeutic Medicineen_HK
dc.subjectAnimal bileen_HK
dc.subjectBile acidsen_HK
dc.subjectCytotoxicityen_HK
dc.subjectHepatocellular carcinomaen_HK
dc.subjectHigh performance liquid chromatography-evaporative light scattering detector systemen_HK
dc.titleChemical and biological analysis of active free and conjugated bile acids in animal bile using HPLC-ELSD and MTT methodsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1792-0981&volume=2&issue=1&spage=125&epage=130&date=2011&atitle=Chemical+and+biological+analysis+of+active+free+and+conjugated+bile+acids+in+animal+bile+by+using+HPLC-ELSD+and+MTT+methods-
dc.identifier.emailFeng, Y: yfeng@hku.hken_HK
dc.identifier.emailZhang, Y: ybzhang@hku.hken_HK
dc.identifier.emailWanMing, NG: kwanmng@hku.hken_HK
dc.identifier.emailLeung, CH: duncanl@hkucc.hku.hken_HK
dc.identifier.emailTong, Y: tongyao@hku.hken_HK
dc.identifier.authorityFeng, Y=rp00466en_HK
dc.identifier.authorityZhang, Y=rp01410en_HK
dc.identifier.authorityWanMing, NG=rp00766en_HK
dc.identifier.authorityLeung, CH=rp00730en_HK
dc.identifier.authorityTong, Y=rp00509en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.3892/etm.2010.178en_HK
dc.identifier.pmcidPMC3440638-
dc.identifier.scopuseid_2-s2.0-78751637141en_HK
dc.identifier.hkuros191221en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78751637141&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume2en_HK
dc.identifier.issue1en_HK
dc.identifier.spage125en_HK
dc.identifier.epage130en_HK
dc.identifier.isiWOS:000286365000019-
dc.publisher.placeGreeceen_HK
dc.identifier.scopusauthoridWang, N=35072317700en_HK
dc.identifier.scopusauthoridFeng, Y=24467969600en_HK
dc.identifier.scopusauthoridXie, TN=36984406000en_HK
dc.identifier.scopusauthoridSu, W=7402010268en_HK
dc.identifier.scopusauthoridZhu, M=36706949300en_HK
dc.identifier.scopusauthoridChow, O=36983856000en_HK
dc.identifier.scopusauthoridZhang, Y=23483121900en_HK
dc.identifier.scopusauthoridWanMing, NG=26026091100en_HK
dc.identifier.scopusauthoridLeung, CH=7402612570en_HK
dc.identifier.scopusauthoridTong, Y=9045384000en_HK

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