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Conference Paper: Development of nucleic acid aptamers against Plasmodium falciparum lactate dehydrogenase for improving the diagnosis of malaria

TitleDevelopment of nucleic acid aptamers against Plasmodium falciparum lactate dehydrogenase for improving the diagnosis of malaria
Authors
Issue Date2011
PublisherThe University of Hong Kong.
Citation
The 2011 Hong Kong Inter-University Biochemistry Postgraduate Symposium, Hong Kong, China, 11 June 2011. How to Cite?
AbstractPrompt confirmation of malaria infection by microscopy and/or rapid diagnostic tests (RDT) is critical to prevent the evolution of drug resistant Plasmodia strains, and also for effective use of artemesin-based combination therapies (ACT). However, malaria diagnosis remains a significant challenge as many malaria endemic regions have inadequate access to microscopy, and antibody-based RDTs are restricted by their poor stability under sustained tropical temperatures and by their cost. The development of nucleic acid aptamers against diagnostic markers for malaria may improve on conventional diagnostic approaches. In this study, DNA aptamers against the diagnostic marker, Plasmodium falciparum lactate dehydrogenase (PfLDH), were identified by Systematic Evolution of Ligands by Exponential Enrichment (SELEX). These DNA aptamers were observed to bind with nanomolar affinity and were highly specific to the Plasmodium LDH and did not bind to the human LDH. This work lays the foundation for further development of rapid diagnostic tests based on novel nucleic acid aptamers for robust and cost-effective malaria diagnosis.
DescriptionOral Presentation: S-H06
Persistent Identifierhttp://hdl.handle.net/10722/137696

 

DC FieldValueLanguage
dc.contributor.authorCheung, YWen_US
dc.contributor.authorTanner, JAen_US
dc.date.accessioned2011-08-26T14:31:42Z-
dc.date.available2011-08-26T14:31:42Z-
dc.date.issued2011en_US
dc.identifier.citationThe 2011 Hong Kong Inter-University Biochemistry Postgraduate Symposium, Hong Kong, China, 11 June 2011.en_US
dc.identifier.urihttp://hdl.handle.net/10722/137696-
dc.descriptionOral Presentation: S-H06-
dc.description.abstractPrompt confirmation of malaria infection by microscopy and/or rapid diagnostic tests (RDT) is critical to prevent the evolution of drug resistant Plasmodia strains, and also for effective use of artemesin-based combination therapies (ACT). However, malaria diagnosis remains a significant challenge as many malaria endemic regions have inadequate access to microscopy, and antibody-based RDTs are restricted by their poor stability under sustained tropical temperatures and by their cost. The development of nucleic acid aptamers against diagnostic markers for malaria may improve on conventional diagnostic approaches. In this study, DNA aptamers against the diagnostic marker, Plasmodium falciparum lactate dehydrogenase (PfLDH), were identified by Systematic Evolution of Ligands by Exponential Enrichment (SELEX). These DNA aptamers were observed to bind with nanomolar affinity and were highly specific to the Plasmodium LDH and did not bind to the human LDH. This work lays the foundation for further development of rapid diagnostic tests based on novel nucleic acid aptamers for robust and cost-effective malaria diagnosis.-
dc.languageengen_US
dc.publisherThe University of Hong Kong.-
dc.relation.ispartofHong Kong Inter-University Biochemistry Postgraduate Symposiumen_US
dc.titleDevelopment of nucleic acid aptamers against Plasmodium falciparum lactate dehydrogenase for improving the diagnosis of malariaen_US
dc.typeConference_Paperen_US
dc.identifier.emailTanner, JA: jatanner@hku.hken_US
dc.identifier.authorityTanner, JA=rp00495en_US
dc.identifier.hkuros190345en_US
dc.description.otherThe 2011 Hong Kong Inter-University Biochemistry Postgraduate Symposium, Hong Kong, China, 11 June 2011.-

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