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Conference Paper: Differential regulation of retinoic acid signaling in adult spinal cord neural progenitors

TitleDifferential regulation of retinoic acid signaling in adult spinal cord neural progenitors
Authors
Issue Date2011
PublisherInternational Society for Stem Cell Research.
Citation
The 9th Annual Meeting of the International Society for Stem Cell Research (ISSCR 2011), Toronto, ON., 15-18 June 2011. In Friday Poster Abstracts of the 9th ISSCR, 2011, p. 56, poster board no. 2028 How to Cite?
AbstractNeural stem cells from the adult subventricular zone (SVZ) are highly heterogeneous, with their position of origin being a key factor in determining the neuronal subtype they can give rise to. Whether this diversity extends to other regions in the adult CNS has not been demonstrated. In vitro studies with directed neuronal differentiation of ES cells suggest that subtype specification may be regulated by the intrinsic positional identity of the ES-derived cell, since altering the positional identity leads to corresponding changes in motor neuron subtype. This limited plasticity suggests the position identity of the original stem cell source is a critical factor for the generation of the desired neuronal subtype. The adult spinal cord consists of endogenous stem/progenitor cells which are activated upon injury, with potential for repair in CNS diseases and spinal cord injury. However, our knowledge and understanding of these cells are limited. Our research is aimed at understanding the properties of endogenous progenitor cells in the adult spinal cord and how they can be utilized for neuronal regeneration. In this study, we identified multiple subpopulations of spinal cord progenitor cells (SCPCs) based on their position along the anterior/posterior (A/P) axis of the adult spinal cord. These subpopulations can be distinguished by the expression of distinct combinatorial Hox genes in a manner reminiscent of their expression in the developing neural tube. Moreover, different progenitor subpopulations display varying cellular properties, such as a higher neurogenic potential and higher neurosphere-forming ability observed in lumbar-derived progenitor cells. We further demonstrate that axial-derived SCPCs are differentially responsive to the neurogenic agent retinoic acid (RA). In the presence of RA, neurogenesis was increased by two-fold during differentiation of cervical-derived spheres, while no increase was observed for lumbar-derived cells. Expression profile analysis of RA signaling components revealed that the RA degrading enzyme cyp26b1, absent in cervical SCPCs but highly expressed in lumbar SCPCs, is likely to regulate RA signaling in these SCPC populations. Our study suggests that different niche factor regimens are required for site-specific neuronal regeneration of endogenous SCPCs from distinct spatial regions.
DescriptionPoster Board No. 2028
Persistent Identifierhttp://hdl.handle.net/10722/137688

 

DC FieldValueLanguage
dc.contributor.authorLeung, Cen_US
dc.contributor.authorChan, SCLen_US
dc.contributor.authorTsang, SLen_US
dc.contributor.authorWu, Wen_US
dc.contributor.authorSham, MHen_US
dc.date.accessioned2011-08-26T14:31:38Z-
dc.date.available2011-08-26T14:31:38Z-
dc.date.issued2011en_US
dc.identifier.citationThe 9th Annual Meeting of the International Society for Stem Cell Research (ISSCR 2011), Toronto, ON., 15-18 June 2011. In Friday Poster Abstracts of the 9th ISSCR, 2011, p. 56, poster board no. 2028en_US
dc.identifier.urihttp://hdl.handle.net/10722/137688-
dc.descriptionPoster Board No. 2028-
dc.description.abstractNeural stem cells from the adult subventricular zone (SVZ) are highly heterogeneous, with their position of origin being a key factor in determining the neuronal subtype they can give rise to. Whether this diversity extends to other regions in the adult CNS has not been demonstrated. In vitro studies with directed neuronal differentiation of ES cells suggest that subtype specification may be regulated by the intrinsic positional identity of the ES-derived cell, since altering the positional identity leads to corresponding changes in motor neuron subtype. This limited plasticity suggests the position identity of the original stem cell source is a critical factor for the generation of the desired neuronal subtype. The adult spinal cord consists of endogenous stem/progenitor cells which are activated upon injury, with potential for repair in CNS diseases and spinal cord injury. However, our knowledge and understanding of these cells are limited. Our research is aimed at understanding the properties of endogenous progenitor cells in the adult spinal cord and how they can be utilized for neuronal regeneration. In this study, we identified multiple subpopulations of spinal cord progenitor cells (SCPCs) based on their position along the anterior/posterior (A/P) axis of the adult spinal cord. These subpopulations can be distinguished by the expression of distinct combinatorial Hox genes in a manner reminiscent of their expression in the developing neural tube. Moreover, different progenitor subpopulations display varying cellular properties, such as a higher neurogenic potential and higher neurosphere-forming ability observed in lumbar-derived progenitor cells. We further demonstrate that axial-derived SCPCs are differentially responsive to the neurogenic agent retinoic acid (RA). In the presence of RA, neurogenesis was increased by two-fold during differentiation of cervical-derived spheres, while no increase was observed for lumbar-derived cells. Expression profile analysis of RA signaling components revealed that the RA degrading enzyme cyp26b1, absent in cervical SCPCs but highly expressed in lumbar SCPCs, is likely to regulate RA signaling in these SCPC populations. Our study suggests that different niche factor regimens are required for site-specific neuronal regeneration of endogenous SCPCs from distinct spatial regions.-
dc.languageengen_US
dc.publisherInternational Society for Stem Cell Research.-
dc.relation.ispartofAnnual Meeting of the International Society for Stem Cell Research, ISSCR 2011en_US
dc.titleDifferential regulation of retinoic acid signaling in adult spinal cord neural progenitorsen_US
dc.typeConference_Paperen_US
dc.identifier.emailLeung, C: leungcar@hkucc.hku.hken_US
dc.identifier.emailTsang, SL: sltsang@hku.hken_US
dc.identifier.emailWu, W: wtwu@hkucc.hku.hken_US
dc.identifier.emailSham, MH: mhsham@hku.hken_US
dc.identifier.authorityWu, W=rp00419en_US
dc.identifier.authoritySham, MH=rp00380en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros190273en_US
dc.identifier.spage56-
dc.identifier.epage56-
dc.description.otherThe 9th Annual Meeting of the International Society for Stem Cell Research (ISSCR 2011), Toronto, ON., 15-18 June 2011. In Friday Poster Abstracts of the 9th ISSCR, 2011, p. 56, poster board no. 2028-

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