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Article: Dendritic cells engineered to express GITRL enhance therapeutic immunity in murine Lewis lung carcinoma
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TitleDendritic cells engineered to express GITRL enhance therapeutic immunity in murine Lewis lung carcinoma
 
AuthorsMa, J1
Wang, S1
Ma, B1
Mao, C1
Tong, J1
Yang, M2
Wu, C1
Jiao, Z1
Lu, L2
Xu, H1
 
Issue Date2011
 
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canlet
 
CitationCancer Letters, 2011, v. 301 n. 2, p. 142-150 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.canlet.2010.11.005
 
AbstractGlucocorticoid-induced tumor necrosis factor receptor and its ligand (GITRL) are critically involved in the regulation of immune response. In this study, we aimed to generate bone marrow-derived dendritic cells (BMDCs) transfected with recombinant adenovirus expressing GITRL (pAd-GITRL-BMDCs) and explore their therapeutic efficacy in murine Lewis lung carcinoma. In vitro, pAd-GITRL-BMDCs greatly enhanced effector T cells proliferation but markedly abrogate the suppression of Treg cells. Moreover, vaccination with pAd-GITRL-BMDCs significantly retarded tumor growth, which was accompanied with increased IFN-γ-producing CD8+ T cells and markedly decreased Treg cells in vivo. These findings suggest GITRL could enhance the immune stimulation of DC and might facilitate the potential development of DCs-based anti-tumor therapies. © 2010 Elsevier Ireland Ltd.
 
ISSN0304-3835
2013 Impact Factor: 5.016
 
DOIhttp://dx.doi.org/10.1016/j.canlet.2010.11.005
 
ISI Accession Number IDWOS:000287554900003
Funding AgencyGrant Number
National Natural Science Foundation of China81072453
30871193
30972748
30910103087
Natural Science Foundation of Jiangsu ProvinceBK2004405
Natural Science Foundation of Jiangsu Province Educational Commission08KJB320002
Health Department Foundation of Jiangsu ProvinceH200952
Jiangsu University
Funding Information:

This study was supported by National Natural Science Foundation of China (Grant Nos. 81072453, 30871193, 30972748, 30910103087), Natural Science Foundation of Jiangsu Province (Grant No. BK2004405), Natural Science Foundation of Jiangsu Province Educational Commission (Grant No. 08KJB320002), Health Department Foundation of Jiangsu Province (Grant No. H200952), and Top Talent Program of Jiangsu University and SCI-Tech Innovation Team of Jiangsu University.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorMa, J
 
dc.contributor.authorWang, S
 
dc.contributor.authorMa, B
 
dc.contributor.authorMao, C
 
dc.contributor.authorTong, J
 
dc.contributor.authorYang, M
 
dc.contributor.authorWu, C
 
dc.contributor.authorJiao, Z
 
dc.contributor.authorLu, L
 
dc.contributor.authorXu, H
 
dc.date.accessioned2011-08-26T14:29:48Z
 
dc.date.available2011-08-26T14:29:48Z
 
dc.date.issued2011
 
dc.description.abstractGlucocorticoid-induced tumor necrosis factor receptor and its ligand (GITRL) are critically involved in the regulation of immune response. In this study, we aimed to generate bone marrow-derived dendritic cells (BMDCs) transfected with recombinant adenovirus expressing GITRL (pAd-GITRL-BMDCs) and explore their therapeutic efficacy in murine Lewis lung carcinoma. In vitro, pAd-GITRL-BMDCs greatly enhanced effector T cells proliferation but markedly abrogate the suppression of Treg cells. Moreover, vaccination with pAd-GITRL-BMDCs significantly retarded tumor growth, which was accompanied with increased IFN-γ-producing CD8+ T cells and markedly decreased Treg cells in vivo. These findings suggest GITRL could enhance the immune stimulation of DC and might facilitate the potential development of DCs-based anti-tumor therapies. © 2010 Elsevier Ireland Ltd.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationCancer Letters, 2011, v. 301 n. 2, p. 142-150 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.canlet.2010.11.005
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.canlet.2010.11.005
 
dc.identifier.epage150
 
dc.identifier.hkuros191305
 
dc.identifier.isiWOS:000287554900003
Funding AgencyGrant Number
National Natural Science Foundation of China81072453
30871193
30972748
30910103087
Natural Science Foundation of Jiangsu ProvinceBK2004405
Natural Science Foundation of Jiangsu Province Educational Commission08KJB320002
Health Department Foundation of Jiangsu ProvinceH200952
Jiangsu University
Funding Information:

This study was supported by National Natural Science Foundation of China (Grant Nos. 81072453, 30871193, 30972748, 30910103087), Natural Science Foundation of Jiangsu Province (Grant No. BK2004405), Natural Science Foundation of Jiangsu Province Educational Commission (Grant No. 08KJB320002), Health Department Foundation of Jiangsu Province (Grant No. H200952), and Top Talent Program of Jiangsu University and SCI-Tech Innovation Team of Jiangsu University.

 
dc.identifier.issn0304-3835
2013 Impact Factor: 5.016
 
dc.identifier.issue2
 
dc.identifier.pmid21186078
 
dc.identifier.scopuseid_2-s2.0-78651462881
 
dc.identifier.spage142
 
dc.identifier.urihttp://hdl.handle.net/10722/137632
 
dc.identifier.volume301
 
dc.languageeng
 
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canlet
 
dc.publisher.placeIreland
 
dc.relation.ispartofCancer Letters
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAdenoviridae - genetics
 
dc.subject.meshAnimals
 
dc.subject.meshBone Marrow Cells - immunology - metabolism
 
dc.subject.meshCD4-Positive T-Lymphocytes - immunology - metabolism
 
dc.subject.meshCD8-Positive T-Lymphocytes - immunology - metabolism
 
dc.subject.meshCarcinoma, Lewis Lung - immunology - pathology - therapy
 
dc.subject.meshCell Line, Tumor
 
dc.subject.meshCell Proliferation
 
dc.subject.meshCells, Cultured
 
dc.subject.meshCoculture Techniques
 
dc.subject.meshDendritic Cells - immunology - metabolism - transplantation
 
dc.subject.meshFlow Cytometry
 
dc.subject.meshFluorescent Antibody Technique
 
dc.subject.meshGenetic Vectors - genetics
 
dc.subject.meshHEK293 Cells
 
dc.subject.meshHumans
 
dc.subject.meshImmunophenotyping
 
dc.subject.meshImmunotherapy, Adoptive
 
dc.subject.meshInterferon-gamma - immunology - metabolism
 
dc.subject.meshMice
 
dc.subject.meshMice, Inbred C57BL
 
dc.subject.meshT-Lymphocytes, Regulatory - immunology - metabolism
 
dc.subject.meshTransduction, Genetic
 
dc.subject.meshTumor Burden - immunology
 
dc.subject.meshTumor Necrosis Factors - genetics - immunology - metabolism
 
dc.titleDendritic cells engineered to express GITRL enhance therapeutic immunity in murine Lewis lung carcinoma
 
dc.typeArticle
 
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Author Affiliations
  1. Jiangsu University
  2. The University of Hong Kong