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Article: Dendritic cells engineered to express GITRL enhance therapeutic immunity in murine Lewis lung carcinoma

TitleDendritic cells engineered to express GITRL enhance therapeutic immunity in murine Lewis lung carcinoma
Authors
Issue Date2011
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canlet
Citation
Cancer Letters, 2011, v. 301 n. 2, p. 142-150 How to Cite?
AbstractGlucocorticoid-induced tumor necrosis factor receptor and its ligand (GITRL) are critically involved in the regulation of immune response. In this study, we aimed to generate bone marrow-derived dendritic cells (BMDCs) transfected with recombinant adenovirus expressing GITRL (pAd-GITRL-BMDCs) and explore their therapeutic efficacy in murine Lewis lung carcinoma. In vitro, pAd-GITRL-BMDCs greatly enhanced effector T cells proliferation but markedly abrogate the suppression of Treg cells. Moreover, vaccination with pAd-GITRL-BMDCs significantly retarded tumor growth, which was accompanied with increased IFN-γ-producing CD8+ T cells and markedly decreased Treg cells in vivo. These findings suggest GITRL could enhance the immune stimulation of DC and might facilitate the potential development of DCs-based anti-tumor therapies. © 2010 Elsevier Ireland Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/137632
ISSN
2014 Impact Factor: 5.621
ISI Accession Number ID
Funding AgencyGrant Number
National Natural Science Foundation of China81072453
30871193
30972748
30910103087
Natural Science Foundation of Jiangsu ProvinceBK2004405
Natural Science Foundation of Jiangsu Province Educational Commission08KJB320002
Health Department Foundation of Jiangsu ProvinceH200952
Jiangsu University
Funding Information:

This study was supported by National Natural Science Foundation of China (Grant Nos. 81072453, 30871193, 30972748, 30910103087), Natural Science Foundation of Jiangsu Province (Grant No. BK2004405), Natural Science Foundation of Jiangsu Province Educational Commission (Grant No. 08KJB320002), Health Department Foundation of Jiangsu Province (Grant No. H200952), and Top Talent Program of Jiangsu University and SCI-Tech Innovation Team of Jiangsu University.

References

 

DC FieldValueLanguage
dc.contributor.authorMa, Jen_HK
dc.contributor.authorWang, Sen_HK
dc.contributor.authorMa, Ben_HK
dc.contributor.authorMao, Cen_HK
dc.contributor.authorTong, Jen_HK
dc.contributor.authorYang, Men_HK
dc.contributor.authorWu, Cen_HK
dc.contributor.authorJiao, Zen_HK
dc.contributor.authorLu, Len_HK
dc.contributor.authorXu, Hen_HK
dc.date.accessioned2011-08-26T14:29:48Z-
dc.date.available2011-08-26T14:29:48Z-
dc.date.issued2011en_HK
dc.identifier.citationCancer Letters, 2011, v. 301 n. 2, p. 142-150en_HK
dc.identifier.issn0304-3835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137632-
dc.description.abstractGlucocorticoid-induced tumor necrosis factor receptor and its ligand (GITRL) are critically involved in the regulation of immune response. In this study, we aimed to generate bone marrow-derived dendritic cells (BMDCs) transfected with recombinant adenovirus expressing GITRL (pAd-GITRL-BMDCs) and explore their therapeutic efficacy in murine Lewis lung carcinoma. In vitro, pAd-GITRL-BMDCs greatly enhanced effector T cells proliferation but markedly abrogate the suppression of Treg cells. Moreover, vaccination with pAd-GITRL-BMDCs significantly retarded tumor growth, which was accompanied with increased IFN-γ-producing CD8+ T cells and markedly decreased Treg cells in vivo. These findings suggest GITRL could enhance the immune stimulation of DC and might facilitate the potential development of DCs-based anti-tumor therapies. © 2010 Elsevier Ireland Ltd.en_HK
dc.languageengen_US
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canleten_HK
dc.relation.ispartofCancer Lettersen_HK
dc.subject.meshAdenoviridae - geneticsen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBone Marrow Cells - immunology - metabolismen_HK
dc.subject.meshCD4-Positive T-Lymphocytes - immunology - metabolismen_HK
dc.subject.meshCD8-Positive T-Lymphocytes - immunology - metabolismen_HK
dc.subject.meshCarcinoma, Lewis Lung - immunology - pathology - therapyen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshCell Proliferationen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshCoculture Techniquesen_HK
dc.subject.meshDendritic Cells - immunology - metabolism - transplantationen_HK
dc.subject.meshFlow Cytometryen_HK
dc.subject.meshFluorescent Antibody Techniqueen_HK
dc.subject.meshGenetic Vectors - geneticsen_HK
dc.subject.meshHEK293 Cellsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunophenotypingen_HK
dc.subject.meshImmunotherapy, Adoptiveen_HK
dc.subject.meshInterferon-gamma - immunology - metabolismen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred C57BLen_HK
dc.subject.meshT-Lymphocytes, Regulatory - immunology - metabolismen_HK
dc.subject.meshTransduction, Geneticen_HK
dc.subject.meshTumor Burden - immunologyen_HK
dc.subject.meshTumor Necrosis Factors - genetics - immunology - metabolismen_HK
dc.titleDendritic cells engineered to express GITRL enhance therapeutic immunity in murine Lewis lung carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.emailLu, L:liweilu@hkucc.hku.hken_HK
dc.identifier.authorityLu, L=rp00477en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.canlet.2010.11.005en_HK
dc.identifier.pmid21186078en_HK
dc.identifier.scopuseid_2-s2.0-78651462881en_HK
dc.identifier.hkuros191305en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78651462881&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume301en_HK
dc.identifier.issue2en_HK
dc.identifier.spage142en_HK
dc.identifier.epage150en_HK
dc.identifier.isiWOS:000287554900003-
dc.publisher.placeIrelanden_HK

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