Article: Dendritic cells engineered to express GITRL enhance therapeutic immunity in murine Lewis lung carcinoma

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TitleDendritic cells engineered to express GITRL enhance therapeutic immunity in murine Lewis lung carcinoma
AuthorsMa, J1
Wang, S1
Ma, B1
Mao, C1
Tong, J1
Yang, M2
Wu, C1
Jiao, Z1
Lu, L2
Xu, H1
Issue Date2011
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canlet
CitationCancer Letters, 2011, v. 301 n. 2, p. 142-150 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.canlet.2010.11.005
AbstractGlucocorticoid-induced tumor necrosis factor receptor and its ligand (GITRL) are critically involved in the regulation of immune response. In this study, we aimed to generate bone marrow-derived dendritic cells (BMDCs) transfected with recombinant adenovirus expressing GITRL (pAd-GITRL-BMDCs) and explore their therapeutic efficacy in murine Lewis lung carcinoma. In vitro, pAd-GITRL-BMDCs greatly enhanced effector T cells proliferation but markedly abrogate the suppression of Treg cells. Moreover, vaccination with pAd-GITRL-BMDCs significantly retarded tumor growth, which was accompanied with increased IFN-γ-producing CD8+ T cells and markedly decreased Treg cells in vivo. These findings suggest GITRL could enhance the immune stimulation of DC and might facilitate the potential development of DCs-based anti-tumor therapies. © 2010 Elsevier Ireland Ltd.
ISSN0304-3835
2011 Impact Factor: 4.238
2011 SCImago Journal Rankings: 0.494
DOIhttp://dx.doi.org/10.1016/j.canlet.2010.11.005
ISI Accession Number IDWOS:000287554900003
Funding AgencyGrant Number
National Natural Science Foundation of China81072453
30871193
30972748
30910103087
Natural Science Foundation of Jiangsu ProvinceBK2004405
Natural Science Foundation of Jiangsu Province Educational Commission08KJB320002
Health Department Foundation of Jiangsu ProvinceH200952
Jiangsu University
Funding Information:

This study was supported by National Natural Science Foundation of China (Grant Nos. 81072453, 30871193, 30972748, 30910103087), Natural Science Foundation of Jiangsu Province (Grant No. BK2004405), Natural Science Foundation of Jiangsu Province Educational Commission (Grant No. 08KJB320002), Health Department Foundation of Jiangsu Province (Grant No. H200952), and Top Talent Program of Jiangsu University and SCI-Tech Innovation Team of Jiangsu University.

ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorMa, J
dc.contributor.authorWang, S
dc.contributor.authorMa, B
dc.contributor.authorMao, C
dc.contributor.authorTong, J
dc.contributor.authorYang, M
dc.contributor.authorWu, C
dc.contributor.authorJiao, Z
dc.contributor.authorLu, L
dc.contributor.authorXu, H
dc.date.accessioned2011-08-26T14:29:48Z
dc.date.available2011-08-26T14:29:48Z
dc.date.issued2011
dc.description.abstractGlucocorticoid-induced tumor necrosis factor receptor and its ligand (GITRL) are critically involved in the regulation of immune response. In this study, we aimed to generate bone marrow-derived dendritic cells (BMDCs) transfected with recombinant adenovirus expressing GITRL (pAd-GITRL-BMDCs) and explore their therapeutic efficacy in murine Lewis lung carcinoma. In vitro, pAd-GITRL-BMDCs greatly enhanced effector T cells proliferation but markedly abrogate the suppression of Treg cells. Moreover, vaccination with pAd-GITRL-BMDCs significantly retarded tumor growth, which was accompanied with increased IFN-γ-producing CD8+ T cells and markedly decreased Treg cells in vivo. These findings suggest GITRL could enhance the immune stimulation of DC and might facilitate the potential development of DCs-based anti-tumor therapies. © 2010 Elsevier Ireland Ltd.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationCancer Letters, 2011, v. 301 n. 2, p. 142-150 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.canlet.2010.11.005
dc.identifier.doihttp://dx.doi.org/10.1016/j.canlet.2010.11.005
dc.identifier.epage150
dc.identifier.hkuros191305
dc.identifier.isiWOS:000287554900003
Funding AgencyGrant Number
National Natural Science Foundation of China81072453
30871193
30972748
30910103087
Natural Science Foundation of Jiangsu ProvinceBK2004405
Natural Science Foundation of Jiangsu Province Educational Commission08KJB320002
Health Department Foundation of Jiangsu ProvinceH200952
Jiangsu University
Funding Information:

This study was supported by National Natural Science Foundation of China (Grant Nos. 81072453, 30871193, 30972748, 30910103087), Natural Science Foundation of Jiangsu Province (Grant No. BK2004405), Natural Science Foundation of Jiangsu Province Educational Commission (Grant No. 08KJB320002), Health Department Foundation of Jiangsu Province (Grant No. H200952), and Top Talent Program of Jiangsu University and SCI-Tech Innovation Team of Jiangsu University.

dc.identifier.issn0304-3835
2011 Impact Factor: 4.238
2011 SCImago Journal Rankings: 0.494
dc.identifier.issue2
dc.identifier.pmid21186078
dc.identifier.scopuseid_2-s2.0-78651462881
dc.identifier.spage142
dc.identifier.urihttp://hdl.handle.net/10722/137632
dc.identifier.volume301
dc.languageeng
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canlet
dc.publisher.placeIreland
dc.relation.ispartofCancer Letters
dc.relation.referencesReferences in Scopus
dc.subject.meshAdenoviridae - genetics
dc.subject.meshAnimals
dc.subject.meshBone Marrow Cells - immunology - metabolism
dc.subject.meshCD4-Positive T-Lymphocytes - immunology - metabolism
dc.subject.meshCD8-Positive T-Lymphocytes - immunology - metabolism
dc.subject.meshCarcinoma, Lewis Lung - immunology - pathology - therapy
dc.subject.meshCell Line, Tumor
dc.subject.meshCell Proliferation
dc.subject.meshCells, Cultured
dc.subject.meshCoculture Techniques
dc.subject.meshDendritic Cells - immunology - metabolism - transplantation
dc.subject.meshFlow Cytometry
dc.subject.meshFluorescent Antibody Technique
dc.subject.meshGenetic Vectors - genetics
dc.subject.meshHEK293 Cells
dc.subject.meshHumans
dc.subject.meshImmunophenotyping
dc.subject.meshImmunotherapy, Adoptive
dc.subject.meshInterferon-gamma - immunology - metabolism
dc.subject.meshMice
dc.subject.meshMice, Inbred C57BL
dc.subject.meshT-Lymphocytes, Regulatory - immunology - metabolism
dc.subject.meshTransduction, Genetic
dc.subject.meshTumor Burden - immunology
dc.subject.meshTumor Necrosis Factors - genetics - immunology - metabolism
dc.titleDendritic cells engineered to express GITRL enhance therapeutic immunity in murine Lewis lung carcinoma
dc.typeArticle
Author Affiliations
  1. Jiangsu University
  2. The University of Hong Kong