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Article: Effect of allogeneic bone marrow-derived mesenchymal stem cells transplantation in a polyI:C-induced primary biliary cirrhosis mouse model
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TitleEffect of allogeneic bone marrow-derived mesenchymal stem cells transplantation in a polyI:C-induced primary biliary cirrhosis mouse model
 
AuthorsWang, D1
Zhang, H1
Liang, J1
Gu, Z1
Ma, X1
Huang, J1
Lin, J1
Hou, Y1
Lu, L2
Sun, L1
 
Issue Date2011
 
PublisherSpringer-Verlag Italia Srl. The Journal's web site is located at http://www.springer.it/libri_libro.asp?id=238
 
CitationClinical And Experimental Medicine, 2011, v. 11 n. 1, p. 25-32 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10238-010-0105-6
 
AbstractPrimary biliary cirrhosis (PBC) is a slowly progressive autoimmune disease of unknown mechanism. We established a PBC animal model by injecting C57BL/6 mice with polyinosinic-polycytidylic acid sodium (polyI:C) to investigate the therapeutic effect of bone marrow-derived mesenchymal stem cells (BM-MSC) on this model. After 6 weeks of MSC infusion, serum aminotransferase and autoimmune antibodies declined, and histological examination by hematoxylin and eosin staining showed significant amelioration of monocytes infiltration around bile ducts of mice treated with BM-MSC. Interestingly, allogeneic BM-MSC transplantation markedly increased CD4+Foxp3+ regulatory T cells in peripheral blood as well as in lymph nodes when analyzed by flow cytometry. Further examination showed serum TGF-β1 increased but IFN-γ decreased significantly in PBC mice treated with MSC, while with no obvious change in IL-10 expression. Our results for the first time suggested that BM-MSC transplantation could regulate systemic immune response and enhance recovery in liver inflammation of PBC mice, raising the possibility for clinical application of allogeneic MSC in treatment of early-stage PBC patients. © 2010 Springer-Verlag.
 
ISSN1591-8890
2012 Impact Factor: 2.397
2012 SCImago Journal Rankings: 0.563
 
DOIhttp://dx.doi.org/10.1007/s10238-010-0105-6
 
ISI Accession Number IDWOS:000286982400004
Funding AgencyGrant Number
Jiangsu Province 135 Talent FoundationRC2007004
Funding Information:

This work was supported by a grant from the Jiangsu Province 135 Talent Foundation (RC2007004).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorWang, D
 
dc.contributor.authorZhang, H
 
dc.contributor.authorLiang, J
 
dc.contributor.authorGu, Z
 
dc.contributor.authorMa, X
 
dc.contributor.authorHuang, J
 
dc.contributor.authorLin, J
 
dc.contributor.authorHou, Y
 
dc.contributor.authorLu, L
 
dc.contributor.authorSun, L
 
dc.date.accessioned2011-08-26T14:29:47Z
 
dc.date.available2011-08-26T14:29:47Z
 
dc.date.issued2011
 
dc.description.abstractPrimary biliary cirrhosis (PBC) is a slowly progressive autoimmune disease of unknown mechanism. We established a PBC animal model by injecting C57BL/6 mice with polyinosinic-polycytidylic acid sodium (polyI:C) to investigate the therapeutic effect of bone marrow-derived mesenchymal stem cells (BM-MSC) on this model. After 6 weeks of MSC infusion, serum aminotransferase and autoimmune antibodies declined, and histological examination by hematoxylin and eosin staining showed significant amelioration of monocytes infiltration around bile ducts of mice treated with BM-MSC. Interestingly, allogeneic BM-MSC transplantation markedly increased CD4+Foxp3+ regulatory T cells in peripheral blood as well as in lymph nodes when analyzed by flow cytometry. Further examination showed serum TGF-β1 increased but IFN-γ decreased significantly in PBC mice treated with MSC, while with no obvious change in IL-10 expression. Our results for the first time suggested that BM-MSC transplantation could regulate systemic immune response and enhance recovery in liver inflammation of PBC mice, raising the possibility for clinical application of allogeneic MSC in treatment of early-stage PBC patients. © 2010 Springer-Verlag.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationClinical And Experimental Medicine, 2011, v. 11 n. 1, p. 25-32 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10238-010-0105-6
 
dc.identifier.citeulike7774631
 
dc.identifier.doihttp://dx.doi.org/10.1007/s10238-010-0105-6
 
dc.identifier.epage32
 
dc.identifier.hkuros191303
 
dc.identifier.isiWOS:000286982400004
Funding AgencyGrant Number
Jiangsu Province 135 Talent FoundationRC2007004
Funding Information:

This work was supported by a grant from the Jiangsu Province 135 Talent Foundation (RC2007004).

 
dc.identifier.issn1591-8890
2012 Impact Factor: 2.397
2012 SCImago Journal Rankings: 0.563
 
dc.identifier.issue1
 
dc.identifier.pmid20661620
 
dc.identifier.scopuseid_2-s2.0-79951949990
 
dc.identifier.spage25
 
dc.identifier.urihttp://hdl.handle.net/10722/137631
 
dc.identifier.volume11
 
dc.languageeng
 
dc.publisherSpringer-Verlag Italia Srl. The Journal's web site is located at http://www.springer.it/libri_libro.asp?id=238
 
dc.publisher.placeItaly
 
dc.relation.ispartofClinical and Experimental Medicine
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAnimals
 
dc.subject.meshAntigens, CD4 - analysis
 
dc.subject.meshAutoantibodies - blood
 
dc.subject.meshFemale
 
dc.subject.meshFlow Cytometry
 
dc.subject.meshForkhead Transcription Factors - analysis
 
dc.subject.meshHistocytochemistry
 
dc.subject.meshInterleukin-10 - biosynthesis
 
dc.subject.meshLiver - pathology
 
dc.subject.meshLiver Cirrhosis, Biliary - chemically induced - therapy
 
dc.subject.meshMale
 
dc.subject.meshMesenchymal Stem Cell Transplantation
 
dc.subject.meshMice
 
dc.subject.meshMice, Inbred C57BL
 
dc.subject.meshPoly I-C - toxicity
 
dc.subject.meshT-Lymphocytes, Regulatory - chemistry - immunology
 
dc.subject.meshTransaminases - blood
 
dc.subject.meshTransplantation, Homologous
 
dc.titleEffect of allogeneic bone marrow-derived mesenchymal stem cells transplantation in a polyI:C-induced primary biliary cirrhosis mouse model
 
dc.typeArticle
 
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Author Affiliations
  1. Nanjing University, School of Medicine
  2. The University of Hong Kong