Article: Effect of allogeneic bone marrow-derived mesenchymal stem cells transplantation in a polyI:C-induced primary biliary cirrhosis mouse model

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TitleEffect of allogeneic bone marrow-derived mesenchymal stem cells transplantation in a polyI:C-induced primary biliary cirrhosis mouse model
AuthorsWang, D1
Zhang, H1
Liang, J1
Gu, Z1
Ma, X1
Huang, J1
Lin, J1
Hou, Y1
Lu, L2
Sun, L1
Issue Date2011
PublisherSpringer-Verlag Italia Srl. The Journal's web site is located at http://www.springer.it/libri_libro.asp?id=238
CitationClinical And Experimental Medicine, 2011, v. 11 n. 1, p. 25-32 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10238-010-0105-6
AbstractPrimary biliary cirrhosis (PBC) is a slowly progressive autoimmune disease of unknown mechanism. We established a PBC animal model by injecting C57BL/6 mice with polyinosinic-polycytidylic acid sodium (polyI:C) to investigate the therapeutic effect of bone marrow-derived mesenchymal stem cells (BM-MSC) on this model. After 6 weeks of MSC infusion, serum aminotransferase and autoimmune antibodies declined, and histological examination by hematoxylin and eosin staining showed significant amelioration of monocytes infiltration around bile ducts of mice treated with BM-MSC. Interestingly, allogeneic BM-MSC transplantation markedly increased CD4+Foxp3+ regulatory T cells in peripheral blood as well as in lymph nodes when analyzed by flow cytometry. Further examination showed serum TGF-β1 increased but IFN-γ decreased significantly in PBC mice treated with MSC, while with no obvious change in IL-10 expression. Our results for the first time suggested that BM-MSC transplantation could regulate systemic immune response and enhance recovery in liver inflammation of PBC mice, raising the possibility for clinical application of allogeneic MSC in treatment of early-stage PBC patients. © 2010 Springer-Verlag.
ISSN1591-8890
2011 Impact Factor: 2.0
2011 SCImago Journal Rankings: 0.137
DOIhttp://dx.doi.org/10.1007/s10238-010-0105-6
ISI Accession Number IDWOS:000286982400004
Funding AgencyGrant Number
Jiangsu Province 135 Talent FoundationRC2007004
Funding Information:

This work was supported by a grant from the Jiangsu Province 135 Talent Foundation (RC2007004).

ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorWang, D
dc.contributor.authorZhang, H
dc.contributor.authorLiang, J
dc.contributor.authorGu, Z
dc.contributor.authorMa, X
dc.contributor.authorHuang, J
dc.contributor.authorLin, J
dc.contributor.authorHou, Y
dc.contributor.authorLu, L
dc.contributor.authorSun, L
dc.date.accessioned2011-08-26T14:29:47Z
dc.date.available2011-08-26T14:29:47Z
dc.date.issued2011
dc.description.abstractPrimary biliary cirrhosis (PBC) is a slowly progressive autoimmune disease of unknown mechanism. We established a PBC animal model by injecting C57BL/6 mice with polyinosinic-polycytidylic acid sodium (polyI:C) to investigate the therapeutic effect of bone marrow-derived mesenchymal stem cells (BM-MSC) on this model. After 6 weeks of MSC infusion, serum aminotransferase and autoimmune antibodies declined, and histological examination by hematoxylin and eosin staining showed significant amelioration of monocytes infiltration around bile ducts of mice treated with BM-MSC. Interestingly, allogeneic BM-MSC transplantation markedly increased CD4+Foxp3+ regulatory T cells in peripheral blood as well as in lymph nodes when analyzed by flow cytometry. Further examination showed serum TGF-β1 increased but IFN-γ decreased significantly in PBC mice treated with MSC, while with no obvious change in IL-10 expression. Our results for the first time suggested that BM-MSC transplantation could regulate systemic immune response and enhance recovery in liver inflammation of PBC mice, raising the possibility for clinical application of allogeneic MSC in treatment of early-stage PBC patients. © 2010 Springer-Verlag.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationClinical And Experimental Medicine, 2011, v. 11 n. 1, p. 25-32 [How to Cite?]
DOI: http://dx.doi.org/10.1007/s10238-010-0105-6
dc.identifier.citeulike7774631
dc.identifier.doihttp://dx.doi.org/10.1007/s10238-010-0105-6
dc.identifier.epage32
dc.identifier.hkuros191303
dc.identifier.isiWOS:000286982400004
Funding AgencyGrant Number
Jiangsu Province 135 Talent FoundationRC2007004
Funding Information:

This work was supported by a grant from the Jiangsu Province 135 Talent Foundation (RC2007004).

dc.identifier.issn1591-8890
2011 Impact Factor: 2.0
2011 SCImago Journal Rankings: 0.137
dc.identifier.issue1
dc.identifier.pmid20661620
dc.identifier.scopuseid_2-s2.0-79951949990
dc.identifier.spage25
dc.identifier.urihttp://hdl.handle.net/10722/137631
dc.identifier.volume11
dc.languageeng
dc.publisherSpringer-Verlag Italia Srl. The Journal's web site is located at http://www.springer.it/libri_libro.asp?id=238
dc.publisher.placeItaly
dc.relation.ispartofClinical and Experimental Medicine
dc.relation.referencesReferences in Scopus
dc.subject.meshAnimals
dc.subject.meshAntigens, CD4 - analysis
dc.subject.meshAutoantibodies - blood
dc.subject.meshFemale
dc.subject.meshFlow Cytometry
dc.subject.meshForkhead Transcription Factors - analysis
dc.subject.meshHistocytochemistry
dc.subject.meshInterleukin-10 - biosynthesis
dc.subject.meshLiver - pathology
dc.subject.meshLiver Cirrhosis, Biliary - chemically induced - therapy
dc.subject.meshMale
dc.subject.meshMesenchymal Stem Cell Transplantation
dc.subject.meshMice
dc.subject.meshMice, Inbred C57BL
dc.subject.meshPoly I-C - toxicity
dc.subject.meshT-Lymphocytes, Regulatory - chemistry - immunology
dc.subject.meshTransaminases - blood
dc.subject.meshTransplantation, Homologous
dc.titleEffect of allogeneic bone marrow-derived mesenchymal stem cells transplantation in a polyI:C-induced primary biliary cirrhosis mouse model
dc.typeArticle
Author Affiliations
  1. Nanjing University, School of Medicine
  2. The University of Hong Kong