Article: MicroRNA-125b suppressesed human liver cancer cell proliferation and metastasis by directly targeting oncogene LIN28B2

File Download Links for fulltext
(May Require Subscription)
Supplementary
  • Basic View
  • Metadata View
  • XML View
TitleMicroRNA-125b suppressesed human liver cancer cell proliferation and metastasis by directly targeting oncogene LIN28B2
AuthorsLiang, L2
Wong, CM1
Ying, Q2
Fan, DNY1
Huang, S2
Ding, J2
Yao, J2
Yan, M2
Li, J2
Yao, M2
Ng, IOL1
He, X2
Issue Date2010
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/
CitationHepatology, 2010, v. 52 n. 5, p. 1731-1740 [How to Cite?]
DOI: http://dx.doi.org/10.1002/hep.23904
AbstractMicroRNAs (miRNAs) are small, noncoding RNAs that can act as oncogenes or tumor suppressors in human cancer. Our previous study showed that miR-125b was a prognostic indicator for patients with hepatocellular carcinoma (HCC), but its functions and exact mechanisms in hepatic carcinogenesis are still unknown. Here we demonstrate that miR-125b suppressed HCC cell growth in vitro and in vivo. Moreover, miR-125b increased p21Cip1/Waf1 expression and arrested cell cycle at G 1 to S transition. In addition, miR-125b inhibited HCC cell migration and invasion. Further studies revealed that LIN28B was a downstream target of miR-125b in HCC cells as miR-125b bound directly to the 3 untranslated region of LIN28B, thus reducing both the messenger RNA and protein levels of LIN28B. Silencing of LIN28B recapitulated the effects of miR-125b overexpression, whereas enforced expression of LIN28B reversed the suppressive effects of miR-125b. Conclusion: These findings indicate that miR-125b exerts tumor-suppressive effects in hepatic carcinogenesis through the suppression of oncogene LIN28B expression and suggest a therapeutic application of miR-125b in HCC. © 2010 American Association for the Study of Liver Diseases.
ISSN0270-9139
2011 Impact Factor: 11.665
2011 SCImago Journal Rankings: 1.278
DOIhttp://dx.doi.org/10.1002/hep.23904
ISI Accession Number IDWOS:000283764800023
Funding AgencyGrant Number
Ministry of Health of China2008ZX10002-017
Science & Technology Commission of Shanghai Municipality07DJ14006
Ministry of Human Resources and Social Security of China2007-170
Funding Information:

Supported by grants from The Ministry of Health of China (2008ZX10002-017), the Science & Technology Commission of Shanghai Municipality (07DJ14006), and the Ministry of Human Resources and Social Security of China (2007-170).

ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorLiang, L
dc.contributor.authorWong, CM
dc.contributor.authorYing, Q
dc.contributor.authorFan, DNY
dc.contributor.authorHuang, S
dc.contributor.authorDing, J
dc.contributor.authorYao, J
dc.contributor.authorYan, M
dc.contributor.authorLi, J
dc.contributor.authorYao, M
dc.contributor.authorNg, IOL
dc.contributor.authorHe, X
dc.date.accessioned2011-08-26T14:29:41Z
dc.date.available2011-08-26T14:29:41Z
dc.date.issued2010
dc.description.abstractMicroRNAs (miRNAs) are small, noncoding RNAs that can act as oncogenes or tumor suppressors in human cancer. Our previous study showed that miR-125b was a prognostic indicator for patients with hepatocellular carcinoma (HCC), but its functions and exact mechanisms in hepatic carcinogenesis are still unknown. Here we demonstrate that miR-125b suppressed HCC cell growth in vitro and in vivo. Moreover, miR-125b increased p21Cip1/Waf1 expression and arrested cell cycle at G 1 to S transition. In addition, miR-125b inhibited HCC cell migration and invasion. Further studies revealed that LIN28B was a downstream target of miR-125b in HCC cells as miR-125b bound directly to the 3 untranslated region of LIN28B, thus reducing both the messenger RNA and protein levels of LIN28B. Silencing of LIN28B recapitulated the effects of miR-125b overexpression, whereas enforced expression of LIN28B reversed the suppressive effects of miR-125b. Conclusion: These findings indicate that miR-125b exerts tumor-suppressive effects in hepatic carcinogenesis through the suppression of oncogene LIN28B expression and suggest a therapeutic application of miR-125b in HCC. © 2010 American Association for the Study of Liver Diseases.
dc.description.naturelink_to_OA_fulltext
dc.identifier.citationHepatology, 2010, v. 52 n. 5, p. 1731-1740 [How to Cite?]
DOI: http://dx.doi.org/10.1002/hep.23904
dc.identifier.citeulike8799928
dc.identifier.doihttp://dx.doi.org/10.1002/hep.23904
dc.identifier.epage1740
dc.identifier.hkuros190839
dc.identifier.isiWOS:000283764800023
Funding AgencyGrant Number
Ministry of Health of China2008ZX10002-017
Science & Technology Commission of Shanghai Municipality07DJ14006
Ministry of Human Resources and Social Security of China2007-170
Funding Information:

Supported by grants from The Ministry of Health of China (2008ZX10002-017), the Science & Technology Commission of Shanghai Municipality (07DJ14006), and the Ministry of Human Resources and Social Security of China (2007-170).

dc.identifier.issn0270-9139
2011 Impact Factor: 11.665
2011 SCImago Journal Rankings: 1.278
dc.identifier.issue5
dc.identifier.pmid20827722
dc.identifier.scopuseid_2-s2.0-78049521995
dc.identifier.spage1731
dc.identifier.urihttp://hdl.handle.net/10722/137627
dc.identifier.volume52
dc.languageeng
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/
dc.publisher.placeUnited States
dc.relation.ispartofHepatology
dc.relation.referencesReferences in Scopus
dc.rightsHepatology. Copyright © John Wiley & Sons, Inc.
dc.subject.meshCell Cycle - drug effects
dc.subject.meshCell Division - drug effects
dc.subject.meshDNA-Binding Proteins - antagonists and inhibitors - drug effects
dc.subject.meshLiver Neoplasms - genetics - pathology
dc.subject.meshMicroRNAs - genetics - pharmacology
dc.titleMicroRNA-125b suppressesed human liver cancer cell proliferation and metastasis by directly targeting oncogene LIN28B2
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Shanghai Jiao Tong University School of Medicine