File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: MicroRNA-125b suppressesed human liver cancer cell proliferation and metastasis by directly targeting oncogene LIN28B2

TitleMicroRNA-125b suppressesed human liver cancer cell proliferation and metastasis by directly targeting oncogene LIN28B2
Authors
Issue Date2010
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/
Citation
Hepatology, 2010, v. 52 n. 5, p. 1731-1740 How to Cite?
AbstractMicroRNAs (miRNAs) are small, noncoding RNAs that can act as oncogenes or tumor suppressors in human cancer. Our previous study showed that miR-125b was a prognostic indicator for patients with hepatocellular carcinoma (HCC), but its functions and exact mechanisms in hepatic carcinogenesis are still unknown. Here we demonstrate that miR-125b suppressed HCC cell growth in vitro and in vivo. Moreover, miR-125b increased p21Cip1/Waf1 expression and arrested cell cycle at G 1 to S transition. In addition, miR-125b inhibited HCC cell migration and invasion. Further studies revealed that LIN28B was a downstream target of miR-125b in HCC cells as miR-125b bound directly to the 3 untranslated region of LIN28B, thus reducing both the messenger RNA and protein levels of LIN28B. Silencing of LIN28B recapitulated the effects of miR-125b overexpression, whereas enforced expression of LIN28B reversed the suppressive effects of miR-125b. Conclusion: These findings indicate that miR-125b exerts tumor-suppressive effects in hepatic carcinogenesis through the suppression of oncogene LIN28B expression and suggest a therapeutic application of miR-125b in HCC. © 2010 American Association for the Study of Liver Diseases.
Persistent Identifierhttp://hdl.handle.net/10722/137627
ISSN
2014 Impact Factor: 11.055
2014 SCImago Journal Rankings: 4.310
ISI Accession Number ID
Funding AgencyGrant Number
Ministry of Health of China2008ZX10002-017
Science & Technology Commission of Shanghai Municipality07DJ14006
Ministry of Human Resources and Social Security of China2007-170
Funding Information:

Supported by grants from The Ministry of Health of China (2008ZX10002-017), the Science & Technology Commission of Shanghai Municipality (07DJ14006), and the Ministry of Human Resources and Social Security of China (2007-170).

References

 

DC FieldValueLanguage
dc.contributor.authorLiang, Len_HK
dc.contributor.authorWong, CMen_HK
dc.contributor.authorYing, Qen_HK
dc.contributor.authorFan, DNYen_HK
dc.contributor.authorHuang, Sen_HK
dc.contributor.authorDing, Jen_HK
dc.contributor.authorYao, Jen_HK
dc.contributor.authorYan, Men_HK
dc.contributor.authorLi, Jen_HK
dc.contributor.authorYao, Men_HK
dc.contributor.authorNg, IOLen_HK
dc.contributor.authorHe, Xen_HK
dc.date.accessioned2011-08-26T14:29:41Z-
dc.date.available2011-08-26T14:29:41Z-
dc.date.issued2010en_HK
dc.identifier.citationHepatology, 2010, v. 52 n. 5, p. 1731-1740en_HK
dc.identifier.issn0270-9139en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137627-
dc.description.abstractMicroRNAs (miRNAs) are small, noncoding RNAs that can act as oncogenes or tumor suppressors in human cancer. Our previous study showed that miR-125b was a prognostic indicator for patients with hepatocellular carcinoma (HCC), but its functions and exact mechanisms in hepatic carcinogenesis are still unknown. Here we demonstrate that miR-125b suppressed HCC cell growth in vitro and in vivo. Moreover, miR-125b increased p21Cip1/Waf1 expression and arrested cell cycle at G 1 to S transition. In addition, miR-125b inhibited HCC cell migration and invasion. Further studies revealed that LIN28B was a downstream target of miR-125b in HCC cells as miR-125b bound directly to the 3 untranslated region of LIN28B, thus reducing both the messenger RNA and protein levels of LIN28B. Silencing of LIN28B recapitulated the effects of miR-125b overexpression, whereas enforced expression of LIN28B reversed the suppressive effects of miR-125b. Conclusion: These findings indicate that miR-125b exerts tumor-suppressive effects in hepatic carcinogenesis through the suppression of oncogene LIN28B expression and suggest a therapeutic application of miR-125b in HCC. © 2010 American Association for the Study of Liver Diseases.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/en_HK
dc.relation.ispartofHepatologyen_HK
dc.rightsHepatology. Copyright © John Wiley & Sons, Inc.-
dc.subject.meshCell Cycle - drug effects-
dc.subject.meshCell Division - drug effects-
dc.subject.meshDNA-Binding Proteins - antagonists and inhibitors - drug effects-
dc.subject.meshLiver Neoplasms - genetics - pathology-
dc.subject.meshMicroRNAs - genetics - pharmacology-
dc.titleMicroRNA-125b suppressesed human liver cancer cell proliferation and metastasis by directly targeting oncogene LIN28B2en_HK
dc.typeArticleen_HK
dc.identifier.emailWong, CM:jackwong@pathology.hku.hken_HK
dc.identifier.emailNg, IOL:iolng@hkucc.hku.hken_HK
dc.identifier.authorityWong, CM=rp00231en_HK
dc.identifier.authorityNg, IOL=rp00335en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/hep.23904en_HK
dc.identifier.pmid20827722en_HK
dc.identifier.scopuseid_2-s2.0-78049521995en_HK
dc.identifier.hkuros190839en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78049521995&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume52en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1731en_HK
dc.identifier.epage1740en_HK
dc.identifier.isiWOS:000283764800023-
dc.publisher.placeUnited Statesen_HK
dc.identifier.citeulike8799928-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats