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Article: Transmissibility of hepatitis B virus (HBV) infection through blood transfusion from blood donors with occult HBV infection
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TitleTransmissibility of hepatitis B virus (HBV) infection through blood transfusion from blood donors with occult HBV infection
 
AuthorsYuen, MF3
Wong, DKH3
Lee, CK1
Tanaka, Y2
Allain, JP5
Fung, J3
Leung, J1
Lin, CK1
Sugiyama, M2
Sugauchi, F2
Mizokami, M4
Lai, CL3
 
Issue Date2011
 
PublisherOxford University Press. The Journal's web site is located at http://www.oxfordjournals.org/our_journals/cid/
 
CitationClinical Infectious Diseases, 2011, v. 52 n. 5, p. 624-632 [How to Cite?]
DOI: http://dx.doi.org/10.1093/cid/ciq247
 
AbstractBackground: Studies of the transmissibility of hepatitis B virus (HBV) in occult hepatitis B (OHB) through blood transfusion are scarce. We aimed to determine the transmissibility of HBV in blood donors with OHB through transfusion in animal and human studies. Methods: Among 217,595 blood donors, 67 donors with OHB were identified. Four chimeric mice populated with human hepatocytes were inoculated with 2 donor serum samples. Serial serum and liver HBV DNA levels were measured. Forty-nine recipients of blood transfusions traced from 10 donors with OHB (9 of whom were positive for antibody to hepatitis B surface antigen [anti-HBs]) were tested for HBV infection. Homology and phylogenetic analyses between the HBV genomic sequences of donors and recipients were performed. Results: Serum HBV DNA was detectable (10 4 copies/mL) in 1 mouse at weeks 5 and 7 after inoculation. Total HBV DNA and HBV replication template (covalently closed circular DNA) and hepatitis B core antigen were detected in the mouse liver. After transfusion, 45 recipients (91.8%) had no HBV infection (ie, they tested negative for hepatitis B surface antigen and HBV DNA). Four tested positive for HBV DNA. In 3 recipients, 83%-86% homology and distant phylogenetic relatedness with their donor HBV excluded transmission through transfusion. The remaining recipient HBV had 95% sequence homology with her donor HBV, compatible with acquisition of HBV infection from the transfusion. High anti-HBs levels in 7 other recipients suggested recent transfusion-related HBV immune response. Conclusions: OHB donor blood infectivity was shown in our animal and human studies. However, the risk of HBV transmission in humans was low, especially from blood products obtained from donors with OHB who were anti-HBs positive. © The Author 2011.
 
ISSN1058-4838
2013 Impact Factor: 9.416
 
DOIhttp://dx.doi.org/10.1093/cid/ciq247
 
ISI Accession Number IDWOS:000286991700016
Funding AgencyGrant Number
Research Grants Council, Hong Kong781108M
Ministry of Health, Labour and Welfare of Japan
Ministry of Education, Culture, Sports, Science and Technology of Japan
LG Life Sciences and FibroGen
Funding Information:

The study was supported by General Research Fund, Research Grants Council, Hong Kong (reference number 781108M) and a grant-in-aid from the Ministry of Health, Labour and Welfare of Japan, and a grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYuen, MF
 
dc.contributor.authorWong, DKH
 
dc.contributor.authorLee, CK
 
dc.contributor.authorTanaka, Y
 
dc.contributor.authorAllain, JP
 
dc.contributor.authorFung, J
 
dc.contributor.authorLeung, J
 
dc.contributor.authorLin, CK
 
dc.contributor.authorSugiyama, M
 
dc.contributor.authorSugauchi, F
 
dc.contributor.authorMizokami, M
 
dc.contributor.authorLai, CL
 
dc.date.accessioned2011-08-26T14:24:11Z
 
dc.date.available2011-08-26T14:24:11Z
 
dc.date.issued2011
 
dc.description.abstractBackground: Studies of the transmissibility of hepatitis B virus (HBV) in occult hepatitis B (OHB) through blood transfusion are scarce. We aimed to determine the transmissibility of HBV in blood donors with OHB through transfusion in animal and human studies. Methods: Among 217,595 blood donors, 67 donors with OHB were identified. Four chimeric mice populated with human hepatocytes were inoculated with 2 donor serum samples. Serial serum and liver HBV DNA levels were measured. Forty-nine recipients of blood transfusions traced from 10 donors with OHB (9 of whom were positive for antibody to hepatitis B surface antigen [anti-HBs]) were tested for HBV infection. Homology and phylogenetic analyses between the HBV genomic sequences of donors and recipients were performed. Results: Serum HBV DNA was detectable (10 4 copies/mL) in 1 mouse at weeks 5 and 7 after inoculation. Total HBV DNA and HBV replication template (covalently closed circular DNA) and hepatitis B core antigen were detected in the mouse liver. After transfusion, 45 recipients (91.8%) had no HBV infection (ie, they tested negative for hepatitis B surface antigen and HBV DNA). Four tested positive for HBV DNA. In 3 recipients, 83%-86% homology and distant phylogenetic relatedness with their donor HBV excluded transmission through transfusion. The remaining recipient HBV had 95% sequence homology with her donor HBV, compatible with acquisition of HBV infection from the transfusion. High anti-HBs levels in 7 other recipients suggested recent transfusion-related HBV immune response. Conclusions: OHB donor blood infectivity was shown in our animal and human studies. However, the risk of HBV transmission in humans was low, especially from blood products obtained from donors with OHB who were anti-HBs positive. © The Author 2011.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationClinical Infectious Diseases, 2011, v. 52 n. 5, p. 624-632 [How to Cite?]
DOI: http://dx.doi.org/10.1093/cid/ciq247
 
dc.identifier.doihttp://dx.doi.org/10.1093/cid/ciq247
 
dc.identifier.eissn1537-6591
 
dc.identifier.epage632
 
dc.identifier.f10009719956
 
dc.identifier.f10009719956
 
dc.identifier.f10009719956
 
dc.identifier.hkuros189849
 
dc.identifier.isiWOS:000286991700016
Funding AgencyGrant Number
Research Grants Council, Hong Kong781108M
Ministry of Health, Labour and Welfare of Japan
Ministry of Education, Culture, Sports, Science and Technology of Japan
LG Life Sciences and FibroGen
Funding Information:

The study was supported by General Research Fund, Research Grants Council, Hong Kong (reference number 781108M) and a grant-in-aid from the Ministry of Health, Labour and Welfare of Japan, and a grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

 
dc.identifier.issn1058-4838
2013 Impact Factor: 9.416
 
dc.identifier.issue5
 
dc.identifier.pmid21245155
 
dc.identifier.scopuseid_2-s2.0-79951939032
 
dc.identifier.spage624
 
dc.identifier.urihttp://hdl.handle.net/10722/137382
 
dc.identifier.volume52
 
dc.languageeng
 
dc.publisherOxford University Press. The Journal's web site is located at http://www.oxfordjournals.org/our_journals/cid/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofClinical Infectious Diseases
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshBlood - virology
 
dc.subject.meshBlood Transfusion - adverse effects
 
dc.subject.meshDNA, Viral - genetics - isolation and purification
 
dc.subject.meshHepatitis B - transmission
 
dc.subject.meshHepatitis B virus - classification - genetics - isolation and purification
 
dc.titleTransmissibility of hepatitis B virus (HBV) infection through blood transfusion from blood donors with occult HBV infection
 
dc.typeArticle
 
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<contributor.author>Tanaka, Y</contributor.author>
<contributor.author>Allain, JP</contributor.author>
<contributor.author>Fung, J</contributor.author>
<contributor.author>Leung, J</contributor.author>
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<contributor.author>Sugiyama, M</contributor.author>
<contributor.author>Sugauchi, F</contributor.author>
<contributor.author>Mizokami, M</contributor.author>
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Author Affiliations
  1. Hong Kong Hospital Authority
  2. Nagoya City University
  3. The University of Hong Kong
  4. International Medical Center of Japan
  5. University of Cambridge