Article: Transmissibility of hepatitis B virus (HBV) infection through blood transfusion from blood donors with occult HBV infection
| Title | Transmissibility of hepatitis B virus (HBV) infection through blood transfusion from blood donors with occult HBV infection | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Authors | Yuen, MF3 Wong, DKH3 Lee, CK1 Tanaka, Y2 Allain, JP5 Fung, J3 Leung, J1 Lin, CK1 Sugiyama, M2 Sugauchi, F2 Mizokami, M4 Lai, CL3 | ||||||||||
| Issue Date | 2011 | ||||||||||
| Publisher | Oxford University Press. The Journal's web site is located at http://www.oxfordjournals.org/our_journals/cid/ | ||||||||||
| Citation | Clinical Infectious Diseases, 2011, v. 52 n. 5, p. 624-632 [How to Cite?] DOI: http://dx.doi.org/10.1093/cid/ciq247 | ||||||||||
| Abstract | Background: Studies of the transmissibility of hepatitis B virus (HBV) in occult hepatitis B (OHB) through blood transfusion are scarce. We aimed to determine the transmissibility of HBV in blood donors with OHB through transfusion in animal and human studies. Methods: Among 217,595 blood donors, 67 donors with OHB were identified. Four chimeric mice populated with human hepatocytes were inoculated with 2 donor serum samples. Serial serum and liver HBV DNA levels were measured. Forty-nine recipients of blood transfusions traced from 10 donors with OHB (9 of whom were positive for antibody to hepatitis B surface antigen [anti-HBs]) were tested for HBV infection. Homology and phylogenetic analyses between the HBV genomic sequences of donors and recipients were performed. Results: Serum HBV DNA was detectable (10 4 copies/mL) in 1 mouse at weeks 5 and 7 after inoculation. Total HBV DNA and HBV replication template (covalently closed circular DNA) and hepatitis B core antigen were detected in the mouse liver. After transfusion, 45 recipients (91.8%) had no HBV infection (ie, they tested negative for hepatitis B surface antigen and HBV DNA). Four tested positive for HBV DNA. In 3 recipients, 83%-86% homology and distant phylogenetic relatedness with their donor HBV excluded transmission through transfusion. The remaining recipient HBV had 95% sequence homology with her donor HBV, compatible with acquisition of HBV infection from the transfusion. High anti-HBs levels in 7 other recipients suggested recent transfusion-related HBV immune response. Conclusions: OHB donor blood infectivity was shown in our animal and human studies. However, the risk of HBV transmission in humans was low, especially from blood products obtained from donors with OHB who were anti-HBs positive. © The Author 2011. | ||||||||||
| ISSN | 1058-4838 2011 Impact Factor: 9.154 2011 SCImago Journal Rankings: 0.821 | ||||||||||
| DOI | http://dx.doi.org/10.1093/cid/ciq247 | ||||||||||
| ISI Accession Number ID | WOS:000286991700016
Funding Information: The study was supported by General Research Fund, Research Grants Council, Hong Kong (reference number 781108M) and a grant-in-aid from the Ministry of Health, Labour and Welfare of Japan, and a grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan. | ||||||||||
| References | References in Scopus |
| dc.contributor.author | Yuen, MF | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| dc.contributor.author | Wong, DKH | ||||||||||
| dc.contributor.author | Lee, CK | ||||||||||
| dc.contributor.author | Tanaka, Y | ||||||||||
| dc.contributor.author | Allain, JP | ||||||||||
| dc.contributor.author | Fung, J | ||||||||||
| dc.contributor.author | Leung, J | ||||||||||
| dc.contributor.author | Lin, CK | ||||||||||
| dc.contributor.author | Sugiyama, M | ||||||||||
| dc.contributor.author | Sugauchi, F | ||||||||||
| dc.contributor.author | Mizokami, M | ||||||||||
| dc.contributor.author | Lai, CL | ||||||||||
| dc.date.accessioned | 2011-08-26T14:24:11Z | ||||||||||
| dc.date.available | 2011-08-26T14:24:11Z | ||||||||||
| dc.date.issued | 2011 | ||||||||||
| dc.description.abstract | Background: Studies of the transmissibility of hepatitis B virus (HBV) in occult hepatitis B (OHB) through blood transfusion are scarce. We aimed to determine the transmissibility of HBV in blood donors with OHB through transfusion in animal and human studies. Methods: Among 217,595 blood donors, 67 donors with OHB were identified. Four chimeric mice populated with human hepatocytes were inoculated with 2 donor serum samples. Serial serum and liver HBV DNA levels were measured. Forty-nine recipients of blood transfusions traced from 10 donors with OHB (9 of whom were positive for antibody to hepatitis B surface antigen [anti-HBs]) were tested for HBV infection. Homology and phylogenetic analyses between the HBV genomic sequences of donors and recipients were performed. Results: Serum HBV DNA was detectable (10 4 copies/mL) in 1 mouse at weeks 5 and 7 after inoculation. Total HBV DNA and HBV replication template (covalently closed circular DNA) and hepatitis B core antigen were detected in the mouse liver. After transfusion, 45 recipients (91.8%) had no HBV infection (ie, they tested negative for hepatitis B surface antigen and HBV DNA). Four tested positive for HBV DNA. In 3 recipients, 83%-86% homology and distant phylogenetic relatedness with their donor HBV excluded transmission through transfusion. The remaining recipient HBV had 95% sequence homology with her donor HBV, compatible with acquisition of HBV infection from the transfusion. High anti-HBs levels in 7 other recipients suggested recent transfusion-related HBV immune response. Conclusions: OHB donor blood infectivity was shown in our animal and human studies. However, the risk of HBV transmission in humans was low, especially from blood products obtained from donors with OHB who were anti-HBs positive. © The Author 2011. | ||||||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||||||
| dc.identifier.citation | Clinical Infectious Diseases, 2011, v. 52 n. 5, p. 624-632 [How to Cite?] DOI: http://dx.doi.org/10.1093/cid/ciq247 | ||||||||||
| dc.identifier.doi | http://dx.doi.org/10.1093/cid/ciq247 | ||||||||||
| dc.identifier.epage | 632 | ||||||||||
| dc.identifier.hkuros | 189849 | ||||||||||
| dc.identifier.isi | WOS:000286991700016
Funding Information: The study was supported by General Research Fund, Research Grants Council, Hong Kong (reference number 781108M) and a grant-in-aid from the Ministry of Health, Labour and Welfare of Japan, and a grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan. | ||||||||||
| dc.identifier.issn | 1058-4838 2011 Impact Factor: 9.154 2011 SCImago Journal Rankings: 0.821 | ||||||||||
| dc.identifier.issue | 5 | ||||||||||
| dc.identifier.pmid | 21245155 | ||||||||||
| dc.identifier.scopus | eid_2-s2.0-79951939032 | ||||||||||
| dc.identifier.spage | 624 | ||||||||||
| dc.identifier.uri | http://hdl.handle.net/10722/137382 | ||||||||||
| dc.identifier.volume | 52 | ||||||||||
| dc.language | eng | ||||||||||
| dc.publisher | Oxford University Press. The Journal's web site is located at http://www.oxfordjournals.org/our_journals/cid/ | ||||||||||
| dc.publisher.place | United States | ||||||||||
| dc.relation.ispartof | Clinical Infectious Diseases | ||||||||||
| dc.relation.references | References in Scopus | ||||||||||
| dc.subject.mesh | Blood - virology | ||||||||||
| dc.subject.mesh | Blood Transfusion - adverse effects | ||||||||||
| dc.subject.mesh | DNA, Viral - genetics - isolation and purification | ||||||||||
| dc.subject.mesh | Hepatitis B - transmission | ||||||||||
| dc.subject.mesh | Hepatitis B virus - classification - genetics - isolation and purification | ||||||||||
| dc.title | Transmissibility of hepatitis B virus (HBV) infection through blood transfusion from blood donors with occult HBV infection | ||||||||||
| dc.type | Article |
Author Affiliations
- Hong Kong Hospital Authority
- Nagoya City University
- The University of Hong Kong
- International Medical Center of Japan
- University of Cambridge