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Article: Transmissibility of hepatitis B virus (HBV) infection through blood transfusion from blood donors with occult HBV infection

TitleTransmissibility of hepatitis B virus (HBV) infection through blood transfusion from blood donors with occult HBV infection
Authors
Issue Date2011
PublisherOxford University Press. The Journal's web site is located at http://www.oxfordjournals.org/our_journals/cid/
Citation
Clinical Infectious Diseases, 2011, v. 52 n. 5, p. 624-632 How to Cite?
Abstract
Background: Studies of the transmissibility of hepatitis B virus (HBV) in occult hepatitis B (OHB) through blood transfusion are scarce. We aimed to determine the transmissibility of HBV in blood donors with OHB through transfusion in animal and human studies. Methods: Among 217,595 blood donors, 67 donors with OHB were identified. Four chimeric mice populated with human hepatocytes were inoculated with 2 donor serum samples. Serial serum and liver HBV DNA levels were measured. Forty-nine recipients of blood transfusions traced from 10 donors with OHB (9 of whom were positive for antibody to hepatitis B surface antigen [anti-HBs]) were tested for HBV infection. Homology and phylogenetic analyses between the HBV genomic sequences of donors and recipients were performed. Results: Serum HBV DNA was detectable (10 4 copies/mL) in 1 mouse at weeks 5 and 7 after inoculation. Total HBV DNA and HBV replication template (covalently closed circular DNA) and hepatitis B core antigen were detected in the mouse liver. After transfusion, 45 recipients (91.8%) had no HBV infection (ie, they tested negative for hepatitis B surface antigen and HBV DNA). Four tested positive for HBV DNA. In 3 recipients, 83%-86% homology and distant phylogenetic relatedness with their donor HBV excluded transmission through transfusion. The remaining recipient HBV had 95% sequence homology with her donor HBV, compatible with acquisition of HBV infection from the transfusion. High anti-HBs levels in 7 other recipients suggested recent transfusion-related HBV immune response. Conclusions: OHB donor blood infectivity was shown in our animal and human studies. However, the risk of HBV transmission in humans was low, especially from blood products obtained from donors with OHB who were anti-HBs positive. © The Author 2011.
Persistent Identifierhttp://hdl.handle.net/10722/137382
ISSN
2013 Impact Factor: 9.416
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants Council, Hong Kong781108M
Ministry of Health, Labour and Welfare of Japan
Ministry of Education, Culture, Sports, Science and Technology of Japan
LG Life Sciences and FibroGen
Funding Information:

The study was supported by General Research Fund, Research Grants Council, Hong Kong (reference number 781108M) and a grant-in-aid from the Ministry of Health, Labour and Welfare of Japan, and a grant-in-aid from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

References

 

Author Affiliations
  1. Hong Kong Hospital Authority
  2. Nagoya City University
  3. The University of Hong Kong
  4. International Medical Center of Japan
  5. University of Cambridge
DC FieldValueLanguage
dc.contributor.authorYuen, MFen_HK
dc.contributor.authorWong, DKHen_HK
dc.contributor.authorLee, CKen_HK
dc.contributor.authorTanaka, Yen_HK
dc.contributor.authorAllain, JPen_HK
dc.contributor.authorFung, Jen_HK
dc.contributor.authorLeung, Jen_HK
dc.contributor.authorLin, CKen_HK
dc.contributor.authorSugiyama, Men_HK
dc.contributor.authorSugauchi, Fen_HK
dc.contributor.authorMizokami, Men_HK
dc.contributor.authorLai, CLen_HK
dc.date.accessioned2011-08-26T14:24:11Z-
dc.date.available2011-08-26T14:24:11Z-
dc.date.issued2011en_HK
dc.identifier.citationClinical Infectious Diseases, 2011, v. 52 n. 5, p. 624-632en_HK
dc.identifier.issn1058-4838en_HK
dc.identifier.urihttp://hdl.handle.net/10722/137382-
dc.description.abstractBackground: Studies of the transmissibility of hepatitis B virus (HBV) in occult hepatitis B (OHB) through blood transfusion are scarce. We aimed to determine the transmissibility of HBV in blood donors with OHB through transfusion in animal and human studies. Methods: Among 217,595 blood donors, 67 donors with OHB were identified. Four chimeric mice populated with human hepatocytes were inoculated with 2 donor serum samples. Serial serum and liver HBV DNA levels were measured. Forty-nine recipients of blood transfusions traced from 10 donors with OHB (9 of whom were positive for antibody to hepatitis B surface antigen [anti-HBs]) were tested for HBV infection. Homology and phylogenetic analyses between the HBV genomic sequences of donors and recipients were performed. Results: Serum HBV DNA was detectable (10 4 copies/mL) in 1 mouse at weeks 5 and 7 after inoculation. Total HBV DNA and HBV replication template (covalently closed circular DNA) and hepatitis B core antigen were detected in the mouse liver. After transfusion, 45 recipients (91.8%) had no HBV infection (ie, they tested negative for hepatitis B surface antigen and HBV DNA). Four tested positive for HBV DNA. In 3 recipients, 83%-86% homology and distant phylogenetic relatedness with their donor HBV excluded transmission through transfusion. The remaining recipient HBV had 95% sequence homology with her donor HBV, compatible with acquisition of HBV infection from the transfusion. High anti-HBs levels in 7 other recipients suggested recent transfusion-related HBV immune response. Conclusions: OHB donor blood infectivity was shown in our animal and human studies. However, the risk of HBV transmission in humans was low, especially from blood products obtained from donors with OHB who were anti-HBs positive. © The Author 2011.en_HK
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://www.oxfordjournals.org/our_journals/cid/en_HK
dc.relation.ispartofClinical Infectious Diseasesen_HK
dc.subject.meshBlood - virology-
dc.subject.meshBlood Transfusion - adverse effects-
dc.subject.meshDNA, Viral - genetics - isolation and purification-
dc.subject.meshHepatitis B - transmission-
dc.subject.meshHepatitis B virus - classification - genetics - isolation and purification-
dc.titleTransmissibility of hepatitis B virus (HBV) infection through blood transfusion from blood donors with occult HBV infectionen_HK
dc.typeArticleen_HK
dc.identifier.emailYuen, MF:mfyuen@hkucc.hku.hken_HK
dc.identifier.emailWong, DKH:danywong@hku.hken_HK
dc.identifier.emailFung, J:jfung@sicklehut.comen_HK
dc.identifier.emailLai, CL:hrmelcl@hku.hken_HK
dc.identifier.authorityYuen, MF=rp00479en_HK
dc.identifier.authorityWong, DKH=rp00492en_HK
dc.identifier.authorityFung, J=rp00518en_HK
dc.identifier.authorityLai, CL=rp00314en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/cid/ciq247en_HK
dc.identifier.pmid21245155en_HK
dc.identifier.scopuseid_2-s2.0-79951939032en_HK
dc.identifier.hkuros189849en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79951939032&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume52en_HK
dc.identifier.issue5en_HK
dc.identifier.spage624en_HK
dc.identifier.epage632en_HK
dc.identifier.eissn1537-6591-
dc.identifier.isiWOS:000286991700016-
dc.publisher.placeUnited Statesen_HK
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dc.identifier.scopusauthoridYuen, MF=7102031955en_HK
dc.identifier.scopusauthoridWong, DKH=7401535819en_HK
dc.identifier.scopusauthoridLee, CK=36087620900en_HK
dc.identifier.scopusauthoridTanaka, Y=35235708000en_HK
dc.identifier.scopusauthoridAllain, JP=7102990257en_HK
dc.identifier.scopusauthoridFung, J=23091109300en_HK
dc.identifier.scopusauthoridLeung, J=37021587000en_HK
dc.identifier.scopusauthoridLin, CK=12752556900en_HK
dc.identifier.scopusauthoridSugiyama, M=8108431700en_HK
dc.identifier.scopusauthoridSugauchi, F=7004837927en_HK
dc.identifier.scopusauthoridMizokami, M=7103318255en_HK
dc.identifier.scopusauthoridLai, CL=7403086396en_HK

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