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Article: Human female reproductive tract epithelial cell culture

TitleHuman female reproductive tract epithelial cell culture
Authors
Issue Date2013
PublisherHumana Press, Inc.
Citation
Methods in Molecular Biology, 2013, v. 945, p. 347-363 How to Cite?
AbstractThe female reproductive system is a complex system. Epithelia of the female reproductive system including the ovaries, the oviduct, and the uterus are important sites for follicular development, ovulation, fertilization, implantation, and embryo development. They are also able to synthesize and secrete various hormones, growth factors, and cytokines, which are essential to women's health, sexuality, and reproduction. Conversely, their dysfunction has been implicated in disorders such as infertility, endometriosis, and many other gynecological diseases, as well as cancer. In this chapter, we describe detailed procedures for establishing and maintaining primary cultures of human ovarian surface epithelium, oviductal epithelium, and endometrium. We also provide protocols for cell immortalization, clonal isolation, and in coculture with stromal cells. These cultures can be useful models for investigating the molecular and cellular functions of these epithelia in both normal and pathological states.
Persistent Identifierhttp://hdl.handle.net/10722/136662
ISSN
2015 SCImago Journal Rankings: 0.549

 

DC FieldValueLanguage
dc.contributor.authorChan, RWSen_US
dc.contributor.authorMak, ASC-
dc.contributor.authorYeung, WSB-
dc.contributor.authorLee, CKF-
dc.contributor.authorCheung, ANY-
dc.contributor.authorNgan, HYS-
dc.contributor.authorWong, AST-
dc.date.accessioned2011-07-27T02:27:17Z-
dc.date.available2011-07-27T02:27:17Z-
dc.date.issued2013en_US
dc.identifier.citationMethods in Molecular Biology, 2013, v. 945, p. 347-363-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/136662-
dc.description.abstractThe female reproductive system is a complex system. Epithelia of the female reproductive system including the ovaries, the oviduct, and the uterus are important sites for follicular development, ovulation, fertilization, implantation, and embryo development. They are also able to synthesize and secrete various hormones, growth factors, and cytokines, which are essential to women's health, sexuality, and reproduction. Conversely, their dysfunction has been implicated in disorders such as infertility, endometriosis, and many other gynecological diseases, as well as cancer. In this chapter, we describe detailed procedures for establishing and maintaining primary cultures of human ovarian surface epithelium, oviductal epithelium, and endometrium. We also provide protocols for cell immortalization, clonal isolation, and in coculture with stromal cells. These cultures can be useful models for investigating the molecular and cellular functions of these epithelia in both normal and pathological states.-
dc.languageengen_US
dc.publisherHumana Press, Inc.en_US
dc.relation.ispartofMethods in Molecular Biology-
dc.rightsThe original publication is available at www.springerlink.com-
dc.subject.meshAntigens, Polyomavirus Transforming - genetics-
dc.subject.meshCell Culture Techniques - methods-
dc.subject.meshCell Proliferation-
dc.subject.meshEpithelial Cells - cytology - metabolism-
dc.subject.meshGenitalia, Female - cytology-
dc.titleHuman female reproductive tract epithelial cell cultureen_US
dc.typeArticleen_US
dc.identifier.emailChan, RWS: rwschan@hku.hken_US
dc.identifier.emailMak, ASC: ascmak@hkucc.hku.hk-
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hk-
dc.identifier.emailLee, CKF: ckflee@hkucc.hku.hk-
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hk-
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hk-
dc.identifier.emailWong, AST: awong1@hku.hk-
dc.identifier.authorityYeung, WSB=rp00331en_US
dc.identifier.doi10.1007/978-1-62703-125-7_21-
dc.identifier.pmid23097117-
dc.identifier.scopuseid_2-s2.0-84870537180-
dc.identifier.hkuros188972en_US
dc.identifier.hkuros205904-
dc.identifier.hkuros221091-
dc.identifier.hkuros238628-
dc.identifier.volume945-
dc.identifier.spage347-
dc.identifier.epage363en_US
dc.publisher.placeUnited States-

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