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Conference Paper: Sirtuin 1 is involved in the generation of induced pluripotent stem cells

TitleSirtuin 1 is involved in the generation of induced pluripotent stem cells
Authors
Issue Date2011
PublisherInternational Society for Stem Cell Research.
Citation
The 9th Annual Meeting of the International Society for Stem Cell Research (ISSCR 2011), Toronto, Canada, 15-18 June 2011. In Friday Abstracts Book, p. 185-186, poster board no. 3196 How to Cite?
AbstractSomatic cells can be reprogrammed into induced pluripotent stem cells (iPSC) by forced-expression of 4 transcription factors. Recent studies show that the reprogramming efficiency can be improved by inclusion of small molecules that modulate epigenetic enzyme activities. We report here that sirtuin 1 (SIRT1), a member of the sirtuin family of NAD+-dependent protein deacetylases, is involved in iPSC formation. Our data indicated that Sirt1 protein levels in human and mouse embryonic stem cells (ESCs) decreased upon differentiation, while that of mouse fibroblasts increased during reprogramming. By using an efficient mouse fibroblasts reprogramming system using doxycycline (DOX) inducible Yamanaka’s transcription factors delivered by piggyBac (PB) transposition, we further showed that Sirt1 was involved in reprogramming. Suppression of Sirt1 level decreased the efficiency of iPSC formation and increased the level of acetylated p53, a known substrate of Sirt1, during reprogramming. Conversely, resveratrol (RSV), an activator of Sirt1, increased the efficiency of iPSC formation. Consistently, the stimulatory activity of RSV on Sirt1 was associated with a reduction of acetylated p53. Furthermore, RSV and Sirt1 siRNA were applied at different periods during reprogramming. They were most effective in influencing the number of iPSC colonies formation in the initiation phase of reprogramming. The results in this study provide support on the possible use of epigenetic regulatory molecules in improving the efficiency of iPSC production.
DescriptionPoster Board Number: 3196
The Conference program's website is located at http://www.isscr.org/home/annual-meeting/past-future-meetings/isscr-9th-annual-meeting-(2011)
Persistent Identifierhttp://hdl.handle.net/10722/136278

 

DC FieldValueLanguage
dc.contributor.authorLee, YLen_US
dc.contributor.authorFong, SWen_US
dc.contributor.authorPeng, Qen_US
dc.contributor.authorLee, KFen_US
dc.contributor.authorNagy, Aen_US
dc.contributor.authorNg, EHYen_US
dc.contributor.authorYeung, WSBen_US
dc.date.accessioned2011-07-27T02:12:10Z-
dc.date.available2011-07-27T02:12:10Z-
dc.date.issued2011en_US
dc.identifier.citationThe 9th Annual Meeting of the International Society for Stem Cell Research (ISSCR 2011), Toronto, Canada, 15-18 June 2011. In Friday Abstracts Book, p. 185-186, poster board no. 3196en_US
dc.identifier.urihttp://hdl.handle.net/10722/136278-
dc.descriptionPoster Board Number: 3196-
dc.descriptionThe Conference program's website is located at http://www.isscr.org/home/annual-meeting/past-future-meetings/isscr-9th-annual-meeting-(2011)-
dc.description.abstractSomatic cells can be reprogrammed into induced pluripotent stem cells (iPSC) by forced-expression of 4 transcription factors. Recent studies show that the reprogramming efficiency can be improved by inclusion of small molecules that modulate epigenetic enzyme activities. We report here that sirtuin 1 (SIRT1), a member of the sirtuin family of NAD+-dependent protein deacetylases, is involved in iPSC formation. Our data indicated that Sirt1 protein levels in human and mouse embryonic stem cells (ESCs) decreased upon differentiation, while that of mouse fibroblasts increased during reprogramming. By using an efficient mouse fibroblasts reprogramming system using doxycycline (DOX) inducible Yamanaka’s transcription factors delivered by piggyBac (PB) transposition, we further showed that Sirt1 was involved in reprogramming. Suppression of Sirt1 level decreased the efficiency of iPSC formation and increased the level of acetylated p53, a known substrate of Sirt1, during reprogramming. Conversely, resveratrol (RSV), an activator of Sirt1, increased the efficiency of iPSC formation. Consistently, the stimulatory activity of RSV on Sirt1 was associated with a reduction of acetylated p53. Furthermore, RSV and Sirt1 siRNA were applied at different periods during reprogramming. They were most effective in influencing the number of iPSC colonies formation in the initiation phase of reprogramming. The results in this study provide support on the possible use of epigenetic regulatory molecules in improving the efficiency of iPSC production.-
dc.languageengen_US
dc.publisherInternational Society for Stem Cell Research.-
dc.relation.ispartofISSCR 9th Annual Meeting 2011en_US
dc.titleSirtuin 1 is involved in the generation of induced pluripotent stem cellsen_US
dc.typeConference_Paperen_US
dc.identifier.emailLee, YL: cherielee@hku.hken_US
dc.identifier.emailFong, SW: szewan11@hku.hken_US
dc.identifier.emailLee, KF: ckflee@hku.hken_US
dc.identifier.emailNg, EHY: nghye@hku.hken_US
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_US
dc.identifier.authorityLee, YL=rp00308en_US
dc.identifier.authorityLee, KF=rp00458en_US
dc.identifier.authorityNg, EHY=rp00426en_US
dc.identifier.authorityYeung, WSB=rp00331en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros187901en_US
dc.identifier.volumeFriday Abstracts Book-
dc.identifier.spage185-
dc.identifier.epage186-
dc.publisher.placeCanada-

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