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Article: Deregulation of microRNA expression occurs early and accumulates in early stages of HBV-associated multistep hepatocarcinogenesis

TitleDeregulation of microRNA expression occurs early and accumulates in early stages of HBV-associated multistep hepatocarcinogenesis
Authors
KeywordsHBV-associated multistep
Hepatocarcinogenesis
microRNA expression
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jhep
Citation
Journal Of Hepatology, 2011, v. 54 n. 6, p. 1177-1184 How to Cite?
AbstractBackground & Aims: Deregulation of microRNAs (miRNAs) plays an important role in human carcinogenesis. However, miRNA deregulation in the pre-malignant lesions and expression changes during multistep hepatocarcinogenesis remain elusive. Methods: In this study, we investigated the expression changes of seven cancer-related miRNAs during the early stages of HBV related hepatocarcinogenesis. miRNA was extracted from formalin fixed paraffin embedded (FFPE) dysplastic nodules (DN), small HCCs, and their corresponding non-tumorous livers. Expression changes of miRNAs were examined by real-time RT-qPCR. Results: We found that down-regulation of miR-145 and miR-199b and up-regulation of miR-224 were frequently observed in pre-malignant DNs and these changes persisted throughout HCC development. Restoration of miR-145 in both HepG2 and Hep3B HCC cells significantly inhibited cell proliferation and reduced cell migration and cell invasion. Furthermore, these inhibitory functions of miR-145 could be substantially reduced by an anti-miR-145 inhibitor. Conclusions: Our results showed that miRNA deregulation was an early event and accumulated throughout the various steps of HBV-associated hepatocarcinogenesis. Our findings also suggest that miR-145 is a candidate tumor suppressive miRNA and may play an important role in HCC development. © 2010 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/135689
ISSN
2015 Impact Factor: 10.59
2015 SCImago Journal Rankings: 4.570
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 1/06C
HKU7/CRG/09
Funding Information:

This study was supported in part by a Hong Kong Research Grants Council Collaborative Research Fund (HKU 1/06C and HKU7/CRG/09). P. Gao was a Cheng Yu Tung Fellow at the University of Hong Kong. IOL Ng is Loke Yew Professor in Pathology.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorGao, Pen_HK
dc.contributor.authorWong, CCLen_HK
dc.contributor.authorTung, EKKen_HK
dc.contributor.authorLee, JMFen_HK
dc.contributor.authorWong, CMen_HK
dc.contributor.authorNg, IOLen_HK
dc.date.accessioned2011-07-27T01:39:29Z-
dc.date.available2011-07-27T01:39:29Z-
dc.date.issued2011en_HK
dc.identifier.citationJournal Of Hepatology, 2011, v. 54 n. 6, p. 1177-1184en_HK
dc.identifier.issn0168-8278en_HK
dc.identifier.urihttp://hdl.handle.net/10722/135689-
dc.description.abstractBackground & Aims: Deregulation of microRNAs (miRNAs) plays an important role in human carcinogenesis. However, miRNA deregulation in the pre-malignant lesions and expression changes during multistep hepatocarcinogenesis remain elusive. Methods: In this study, we investigated the expression changes of seven cancer-related miRNAs during the early stages of HBV related hepatocarcinogenesis. miRNA was extracted from formalin fixed paraffin embedded (FFPE) dysplastic nodules (DN), small HCCs, and their corresponding non-tumorous livers. Expression changes of miRNAs were examined by real-time RT-qPCR. Results: We found that down-regulation of miR-145 and miR-199b and up-regulation of miR-224 were frequently observed in pre-malignant DNs and these changes persisted throughout HCC development. Restoration of miR-145 in both HepG2 and Hep3B HCC cells significantly inhibited cell proliferation and reduced cell migration and cell invasion. Furthermore, these inhibitory functions of miR-145 could be substantially reduced by an anti-miR-145 inhibitor. Conclusions: Our results showed that miRNA deregulation was an early event and accumulated throughout the various steps of HBV-associated hepatocarcinogenesis. Our findings also suggest that miR-145 is a candidate tumor suppressive miRNA and may play an important role in HCC development. © 2010 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jhepen_HK
dc.relation.ispartofJournal of Hepatologyen_HK
dc.subjectHBV-associated multistepen_HK
dc.subjectHepatocarcinogenesisen_HK
dc.subjectmicroRNA expressionen_HK
dc.titleDeregulation of microRNA expression occurs early and accumulates in early stages of HBV-associated multistep hepatocarcinogenesisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0168-8278&volume=54&issue=6&spage=1177&epage=1184&date=2011&atitle=Deregulation+of+microRNA+expression+occurs+early+and+accumulates+in+early+stages+of+HBV-associated+multistep+hepatocarcinogenesis-
dc.identifier.emailWong, CCL:carmencl@pathology.hku.hken_HK
dc.identifier.emailWong, CM:jackwong@pathology.hku.hken_HK
dc.identifier.emailNg, IOL:iolng@hkucc.hku.hken_HK
dc.identifier.authorityWong, CCL=rp01602en_HK
dc.identifier.authorityWong, CM=rp00231en_HK
dc.identifier.authorityNg, IOL=rp00335en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jhep.2010.09.023en_HK
dc.identifier.pmid21145831-
dc.identifier.scopuseid_2-s2.0-79956081820en_HK
dc.identifier.hkuros187401en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79956081820&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume54en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1177en_HK
dc.identifier.epage1184en_HK
dc.identifier.isiWOS:000291523000015-
dc.publisher.placeNetherlandsen_HK
dc.relation.projectMolecular pathology of liver cancer - a multidisciplinary study-
dc.identifier.scopusauthoridGao, P=35974917600en_HK
dc.identifier.scopusauthoridWong, CCL=24823630000en_HK
dc.identifier.scopusauthoridTung, EKK=7003519614en_HK
dc.identifier.scopusauthoridLee, JMF=39561284400en_HK
dc.identifier.scopusauthoridWong, CM=16314668400en_HK
dc.identifier.scopusauthoridNg, IOL=7102753722en_HK
dc.identifier.citeulike8186085-

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