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Article: Identification of cells with colony-forming activity, self-renewal capacity, and multipotency in ovarian endometriosis

TitleIdentification of cells with colony-forming activity, self-renewal capacity, and multipotency in ovarian endometriosis
Authors
Issue Date2011
PublisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.org
Citation
American Journal Of Pathology, 2011, v. 178 n. 6, p. 2832-2844 How to Cite?
Abstract
Endometriosis, the growth of endometrial tissue outside the uterine cavity, is a common gynecological disorder affecting 10% to 15% of women in their reproductive years. Retrograde menstrual shedding containing endometrial stem/progenitor cells has been postulated to be involved in its pathogenesis. In this study, we identified putative endometriotic stem/ progenitor cells by their colony-forming potential, self-renewal capacity, and multipotency. Purified epithelial and stromal cells isolated from ovarian endometriotic cysts formed large and small colony-forming units (CFUs) in clonogenic assay. The colony-forming activity of epithelial and stromal cells was found to differ greatly between autologous endometrium and ovarian endometrioma samples. The large CFUs could propagate more than the small CFUs. The endometriotic epithelial small CFUs expressed epithelial markers (epithelial cell adhesion molecule, cytokeratin, and α6 integrin); only occasional large CFUs expressed α6 integrin. Aside from the expression of fibroblast markers, stromal CFUs also expressed three somatic stem cell markers: sal-like 4, CD133, and Musashi-1. Endometriotic stromal cells derived from large CFUs could differentiate into four mesenchymal lineages when cultured in the respective inducing-media, as determined by histochemical staining and RT-PCR of lineage specific markers. These findings demonstrate that ovarian endometrioma contains a subset of cells displaying somatic stem cell properties.
Persistent Identifierhttp://hdl.handle.net/10722/135668
ISSN
2013 Impact Factor: 4.602
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Research Grants CouncilHKU 7822/09M
Funding Information:

Supported by a grant from the General Research Fund (GRF), Research Grants Council (HKU 7822/09M to W.S.B.Y.).

References

 

Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorChan, RWSen_HK
dc.contributor.authorNg, EHYen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2011-07-27T01:39:08Z-
dc.date.available2011-07-27T01:39:08Z-
dc.date.issued2011en_HK
dc.identifier.citationAmerican Journal Of Pathology, 2011, v. 178 n. 6, p. 2832-2844en_HK
dc.identifier.issn0002-9440en_HK
dc.identifier.urihttp://hdl.handle.net/10722/135668-
dc.description.abstractEndometriosis, the growth of endometrial tissue outside the uterine cavity, is a common gynecological disorder affecting 10% to 15% of women in their reproductive years. Retrograde menstrual shedding containing endometrial stem/progenitor cells has been postulated to be involved in its pathogenesis. In this study, we identified putative endometriotic stem/ progenitor cells by their colony-forming potential, self-renewal capacity, and multipotency. Purified epithelial and stromal cells isolated from ovarian endometriotic cysts formed large and small colony-forming units (CFUs) in clonogenic assay. The colony-forming activity of epithelial and stromal cells was found to differ greatly between autologous endometrium and ovarian endometrioma samples. The large CFUs could propagate more than the small CFUs. The endometriotic epithelial small CFUs expressed epithelial markers (epithelial cell adhesion molecule, cytokeratin, and α6 integrin); only occasional large CFUs expressed α6 integrin. Aside from the expression of fibroblast markers, stromal CFUs also expressed three somatic stem cell markers: sal-like 4, CD133, and Musashi-1. Endometriotic stromal cells derived from large CFUs could differentiate into four mesenchymal lineages when cultured in the respective inducing-media, as determined by histochemical staining and RT-PCR of lineage specific markers. These findings demonstrate that ovarian endometrioma contains a subset of cells displaying somatic stem cell properties.en_HK
dc.languageengen_US
dc.publisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.orgen_HK
dc.relation.ispartofAmerican Journal of Pathologyen_HK
dc.titleIdentification of cells with colony-forming activity, self-renewal capacity, and multipotency in ovarian endometriosisen_HK
dc.typeArticleen_HK
dc.identifier.emailNg, EHY:nghye@hkucc.hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityNg, EHY=rp00426en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.ajpath.2011.02.025en_HK
dc.identifier.pmid21641404en_HK
dc.identifier.pmcidPMC3123988-
dc.identifier.scopuseid_2-s2.0-79959380907en_HK
dc.identifier.hkuros185956en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79959380907&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume178en_HK
dc.identifier.issue6en_HK
dc.identifier.spage2832en_HK
dc.identifier.epage2844en_HK
dc.identifier.eissn1525-2191-
dc.identifier.isiWOS:000298306900038-
dc.publisher.placeUnited Statesen_HK
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dc.identifier.scopusauthoridChan, RWS=8938013400en_HK
dc.identifier.scopusauthoridNg, EHY=35238184300en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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