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Article: Identification of cells with colony-forming activity, self-renewal capacity, and multipotency in ovarian endometriosis
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TitleIdentification of cells with colony-forming activity, self-renewal capacity, and multipotency in ovarian endometriosis
 
AuthorsChan, RWS1 2
Ng, EHY1
Yeung, WSB1
 
Issue Date2011
 
PublisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.org
 
CitationAmerican Journal Of Pathology, 2011, v. 178 n. 6, p. 2832-2844 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ajpath.2011.02.025
 
AbstractEndometriosis, the growth of endometrial tissue outside the uterine cavity, is a common gynecological disorder affecting 10% to 15% of women in their reproductive years. Retrograde menstrual shedding containing endometrial stem/progenitor cells has been postulated to be involved in its pathogenesis. In this study, we identified putative endometriotic stem/ progenitor cells by their colony-forming potential, self-renewal capacity, and multipotency. Purified epithelial and stromal cells isolated from ovarian endometriotic cysts formed large and small colony-forming units (CFUs) in clonogenic assay. The colony-forming activity of epithelial and stromal cells was found to differ greatly between autologous endometrium and ovarian endometrioma samples. The large CFUs could propagate more than the small CFUs. The endometriotic epithelial small CFUs expressed epithelial markers (epithelial cell adhesion molecule, cytokeratin, and α6 integrin); only occasional large CFUs expressed α6 integrin. Aside from the expression of fibroblast markers, stromal CFUs also expressed three somatic stem cell markers: sal-like 4, CD133, and Musashi-1. Endometriotic stromal cells derived from large CFUs could differentiate into four mesenchymal lineages when cultured in the respective inducing-media, as determined by histochemical staining and RT-PCR of lineage specific markers. These findings demonstrate that ovarian endometrioma contains a subset of cells displaying somatic stem cell properties.
 
ISSN0002-9440
2012 Impact Factor: 4.522
2012 SCImago Journal Rankings: 2.148
 
DOIhttp://dx.doi.org/10.1016/j.ajpath.2011.02.025
 
ISI Accession Number IDWOS:000298306900038
Funding AgencyGrant Number
Research Grants CouncilHKU 7822/09M
Funding Information:

Supported by a grant from the General Research Fund (GRF), Research Grants Council (HKU 7822/09M to W.S.B.Y.).

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChan, RWS
 
dc.contributor.authorNg, EHY
 
dc.contributor.authorYeung, WSB
 
dc.date.accessioned2011-07-27T01:39:08Z
 
dc.date.available2011-07-27T01:39:08Z
 
dc.date.issued2011
 
dc.description.abstractEndometriosis, the growth of endometrial tissue outside the uterine cavity, is a common gynecological disorder affecting 10% to 15% of women in their reproductive years. Retrograde menstrual shedding containing endometrial stem/progenitor cells has been postulated to be involved in its pathogenesis. In this study, we identified putative endometriotic stem/ progenitor cells by their colony-forming potential, self-renewal capacity, and multipotency. Purified epithelial and stromal cells isolated from ovarian endometriotic cysts formed large and small colony-forming units (CFUs) in clonogenic assay. The colony-forming activity of epithelial and stromal cells was found to differ greatly between autologous endometrium and ovarian endometrioma samples. The large CFUs could propagate more than the small CFUs. The endometriotic epithelial small CFUs expressed epithelial markers (epithelial cell adhesion molecule, cytokeratin, and α6 integrin); only occasional large CFUs expressed α6 integrin. Aside from the expression of fibroblast markers, stromal CFUs also expressed three somatic stem cell markers: sal-like 4, CD133, and Musashi-1. Endometriotic stromal cells derived from large CFUs could differentiate into four mesenchymal lineages when cultured in the respective inducing-media, as determined by histochemical staining and RT-PCR of lineage specific markers. These findings demonstrate that ovarian endometrioma contains a subset of cells displaying somatic stem cell properties.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationAmerican Journal Of Pathology, 2011, v. 178 n. 6, p. 2832-2844 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ajpath.2011.02.025
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.ajpath.2011.02.025
 
dc.identifier.eissn1525-2191
 
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dc.identifier.hkuros185956
 
dc.identifier.isiWOS:000298306900038
Funding AgencyGrant Number
Research Grants CouncilHKU 7822/09M
Funding Information:

Supported by a grant from the General Research Fund (GRF), Research Grants Council (HKU 7822/09M to W.S.B.Y.).

 
dc.identifier.issn0002-9440
2012 Impact Factor: 4.522
2012 SCImago Journal Rankings: 2.148
 
dc.identifier.issue6
 
dc.identifier.pmid21641404
 
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dc.identifier.urihttp://hdl.handle.net/10722/135668
 
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dc.languageeng
 
dc.publisherAmerican Society for Investigative Pathology. The Journal's web site is located at http://www.amjpathol.org
 
dc.publisher.placeUnited States
 
dc.relation.ispartofAmerican Journal of Pathology
 
dc.relation.referencesReferences in Scopus
 
dc.titleIdentification of cells with colony-forming activity, self-renewal capacity, and multipotency in ovarian endometriosis
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong