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Article: Hormonal regulation of endometrial olfactomedin expression and its suppressive effect on spheroid attachment onto endometrial epithelial cells

TitleHormonal regulation of endometrial olfactomedin expression and its suppressive effect on spheroid attachment onto endometrial epithelial cells
Authors
Keywordsadhesions
attachment
endometrial receptivity
olfactomedin
spheroids
Issue Date2011
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 2011, v. 26 n. 1, p. 167-175 How to Cite?
AbstractBackground Olfactomedin (Olfm) is a member of a diverse group of extracellular matrix proteins important for neuronal growth. Recent microarray studies identified Olfm as one of the down-regulated transcripts in receptive endometrium at the time of embryo attachment and implantation. However, the underlying molecular mechanisms that govern Olfm expression and its effect on embryo attachment and implantation remain unknown. Methods The expression of Olfm in the human endometrium was investigated by real-time PCR, western blotting and immunohistochemistry on human endometrial biopsies from natural and ovarian stimulated cycles. To investigate the function of Olfm in trophoblastendometrial cell attachment, an in vitro spheroid-endometrial cell co-culture study was performed. Results Human endometrial Olfactomedin-1 and -2(Olfm-1 and -2) transcripts decreased significantly from the proliferative to the secretory phases of the menstrual cycle. Olfm protein was strongly expressed in the luminal and glandular epithelium and moderately in the stromal cells of human endometria. Ovarian stimulation significantly decreased (P < 0.05) the expression of endometrial Olfm-1 and -2 transcripts in patients receiving IVF treatment when compared with those in the natural cycle. Importantly, recombinant Olfm-1 suppressed JAr spheroid attachment onto Ishikawa cells and this was not associated with changes of β-catenin and E-cadherin expression in trophoblast and endometrial cells. Conclusions Decreased expression of Olfm during the receptive phase of the endometrium may allow successful trophoblast attachment for implantation. © 2010 The Author.
Persistent Identifierhttp://hdl.handle.net/10722/135636
ISSN
2015 Impact Factor: 4.621
2015 SCImago Journal Rankings: 2.271
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong
Hong Kong Research Grants CouncilHKU 7514/05M
Committee on Research and Conference
Funding Information:

This work was supported in part by grants from the Committee on Research and Conference Grants, The University of Hong Kong to KFL and the Hong Kong Research Grants Council to PC Ho (HKU 7514/05M).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorKodithuwakku, SPen_HK
dc.contributor.authorNg, PYen_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorNg, EHYen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorLee, KFen_HK
dc.date.accessioned2011-07-27T01:38:12Z-
dc.date.available2011-07-27T01:38:12Z-
dc.date.issued2011en_HK
dc.identifier.citationHuman Reproduction, 2011, v. 26 n. 1, p. 167-175en_HK
dc.identifier.issn0268-1161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/135636-
dc.description.abstractBackground Olfactomedin (Olfm) is a member of a diverse group of extracellular matrix proteins important for neuronal growth. Recent microarray studies identified Olfm as one of the down-regulated transcripts in receptive endometrium at the time of embryo attachment and implantation. However, the underlying molecular mechanisms that govern Olfm expression and its effect on embryo attachment and implantation remain unknown. Methods The expression of Olfm in the human endometrium was investigated by real-time PCR, western blotting and immunohistochemistry on human endometrial biopsies from natural and ovarian stimulated cycles. To investigate the function of Olfm in trophoblastendometrial cell attachment, an in vitro spheroid-endometrial cell co-culture study was performed. Results Human endometrial Olfactomedin-1 and -2(Olfm-1 and -2) transcripts decreased significantly from the proliferative to the secretory phases of the menstrual cycle. Olfm protein was strongly expressed in the luminal and glandular epithelium and moderately in the stromal cells of human endometria. Ovarian stimulation significantly decreased (P < 0.05) the expression of endometrial Olfm-1 and -2 transcripts in patients receiving IVF treatment when compared with those in the natural cycle. Importantly, recombinant Olfm-1 suppressed JAr spheroid attachment onto Ishikawa cells and this was not associated with changes of β-catenin and E-cadherin expression in trophoblast and endometrial cells. Conclusions Decreased expression of Olfm during the receptive phase of the endometrium may allow successful trophoblast attachment for implantation. © 2010 The Author.en_HK
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_HK
dc.relation.ispartofHuman Reproductionen_HK
dc.rightsThis is a pre-copy-editing, author-produced PDF of an article accepted for publication in Human Reproduction following peer review. The definitive publisher-authenticated version Human Reproduction, 2011, v. 26 n. 1, p. 167-175 is available online at: http://humrep.oxfordjournals.org/content/26/1/167-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectadhesionsen_HK
dc.subjectattachmenten_HK
dc.subjectendometrial receptivityen_HK
dc.subjectolfactomedinen_HK
dc.subjectspheroidsen_HK
dc.subject.meshAmino Acid Sequence-
dc.subject.meshEmbryo Implantation-
dc.subject.meshEndometrium - metabolism-
dc.subject.meshExtracellular Matrix Proteins - chemistry - genetics - metabolism-
dc.subject.meshGlycoproteins - chemistry - genetics - metabolism-
dc.titleHormonal regulation of endometrial olfactomedin expression and its suppressive effect on spheroid attachment onto endometrial epithelial cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0268-1161&volume=26&issue=1&spage=167&epage=175&date=2011&atitle=Hormonal+regulation+of+endometrial+olfactomedin+expression+and+its+suppressive+effect+on+spheroid+attachment+onto+endometrial+epithelial+cells-
dc.identifier.emailNg, EHY:nghye@hkucc.hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.emailLee, KF:ckflee@hku.hken_HK
dc.identifier.authorityNg, EHY=rp00426en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1093/humrep/deq298en_HK
dc.identifier.pmid21106493-
dc.identifier.scopuseid_2-s2.0-78650710504en_HK
dc.identifier.hkuros187018en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78650710504&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume26en_HK
dc.identifier.issue1en_HK
dc.identifier.spage167en_HK
dc.identifier.epage175en_HK
dc.identifier.eissn1460-2350-
dc.identifier.isiWOS:000285627200021-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectRole of olfactomedin in implantation-
dc.identifier.scopusauthoridKodithuwakku, SP=8939085200en_HK
dc.identifier.scopusauthoridNg, PY=24174994900en_HK
dc.identifier.scopusauthoridLiu, Y=23134878300en_HK
dc.identifier.scopusauthoridNg, EHY=35238184300en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK

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