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Article: In Vivo Lipid Profiling Using Proton Magnetic Resonance Spectroscopy in an Experimental Liver Fibrosis Model

TitleIn Vivo Lipid Profiling Using Proton Magnetic Resonance Spectroscopy in an Experimental Liver Fibrosis Model
Authors
KeywordsCarbon tetrachloride (CCl 4)
Lipid
Liver fibrosis
Proton magnetic resonance spectroscopy ( 1H MRS)
Saturated fatty acid
Unsaturated fatty acid
Issue Date2011
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/arad
Citation
Academic Radiology, 2011, v. 18 n. 3, p. 377-383 How to Cite?
AbstractRationale and Objectives: The aim of this study was to characterize early hepatic lipid changes in an experimental model of liver fibrosis using proton ( 1H) magnetic resonance spectroscopy (MRS) at high magnetic field in vivo. Materials and Methods: Liver fibrosis was induced in 12 Sprague-Dawley rats by twice-weekly carbon tetrachloride (CCl 4) administration up to 4 weeks. Eight normal rats were used as controls. Single-voxel 1H MRS experiments were performed at 7 Tesla to measure signal integrals of various lipid peaks including -CH 3, (-CH 2-) n, -CH 2-C=C-CH 2-, =C-CH 2-C= and -CH=CH- at 0.9, 1.3, 2.0, 2.8, and 5.3 ppm, respectively, and peak from choline-containing compounds (CCC) at 3.2 ppm. Total lipid, total saturated fatty acid, total unsaturated fatty acid, total unsaturated bond, polyunsaturated bond, and CCC indices were quantified. Results: Significant increases (P<.01) in total lipid and total saturated fatty acid indices were found in animals with CCl 4-induced fibrosis as compared with normal animals. In addition, total unsaturated bond and polyunsaturated bond indices of animals at 4 weeks after CCl 4 insult were significantly higher than (P<.01 and P<.05, respectively) those of normal animals and animals at 2 weeks following insult; whereas there was only significant increase (P<.01) in total unsaturated fatty acid index in animals with 4-week CCl 4 insult as compared with normal animals. Conclusion: The hepatic lipid changes in CCl 4-induced experimental fibrosis model were documented in vivo and longitudinally using 1H MRS at 7 Tesla. The experimental findings suggested that total saturated fatty acid increase contributed mainly to the total lipid increase in animals with CCl 4 insult. This study also demonstrated the potential value of high field MRS to resolve lipid composition and alterations in liver fibrosis. © 2011 AUR.
Persistent Identifierhttp://hdl.handle.net/10722/135548
ISSN
2015 Impact Factor: 1.966
2015 SCImago Journal Rankings: 1.008
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Grant CouncilGRF HKU7808/09M
Funding Information:

From the Laboratory of Biomedical Imaging and Signal Processing (J.S.C., S.J.F., D.S.G., A.M.C., J.Y., E.X.W.), Departments of Electrical and Electronic Engineering (J.S.C., S.J.F., D.S.G., A.M.C., J.Y., E.X.W.), Surgery (KM.), and Anatomy (E.X.W.), The University of Hong Kong, Pokfulam, Hong Kong SAR, China; Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA (J.S.C); Department of Diagnostic Radiology of the First Affiliated Hospital, School of Medicine of Xi'an Jiaotong University, Xi'an, Shannxi Province, China (J.Y.). Received September 12, 2010; accepted October 29, 2010. Supported by the Hong Kong Grant Council (GRF HKU7808/09M). Address correspondence to: E.X.W. e-mail: ewu@eee.hku.hk

References

 

DC FieldValueLanguage
dc.contributor.authorCheung, JSen_HK
dc.contributor.authorFan, SJen_HK
dc.contributor.authorGao, DSen_HK
dc.contributor.authorChow, AMen_HK
dc.contributor.authorYang, Jen_HK
dc.contributor.authorMan, Ken_HK
dc.contributor.authorWu, EXen_HK
dc.date.accessioned2011-07-27T01:36:54Z-
dc.date.available2011-07-27T01:36:54Z-
dc.date.issued2011en_HK
dc.identifier.citationAcademic Radiology, 2011, v. 18 n. 3, p. 377-383en_HK
dc.identifier.issn1076-6332en_HK
dc.identifier.urihttp://hdl.handle.net/10722/135548-
dc.description.abstractRationale and Objectives: The aim of this study was to characterize early hepatic lipid changes in an experimental model of liver fibrosis using proton ( 1H) magnetic resonance spectroscopy (MRS) at high magnetic field in vivo. Materials and Methods: Liver fibrosis was induced in 12 Sprague-Dawley rats by twice-weekly carbon tetrachloride (CCl 4) administration up to 4 weeks. Eight normal rats were used as controls. Single-voxel 1H MRS experiments were performed at 7 Tesla to measure signal integrals of various lipid peaks including -CH 3, (-CH 2-) n, -CH 2-C=C-CH 2-, =C-CH 2-C= and -CH=CH- at 0.9, 1.3, 2.0, 2.8, and 5.3 ppm, respectively, and peak from choline-containing compounds (CCC) at 3.2 ppm. Total lipid, total saturated fatty acid, total unsaturated fatty acid, total unsaturated bond, polyunsaturated bond, and CCC indices were quantified. Results: Significant increases (P<.01) in total lipid and total saturated fatty acid indices were found in animals with CCl 4-induced fibrosis as compared with normal animals. In addition, total unsaturated bond and polyunsaturated bond indices of animals at 4 weeks after CCl 4 insult were significantly higher than (P<.01 and P<.05, respectively) those of normal animals and animals at 2 weeks following insult; whereas there was only significant increase (P<.01) in total unsaturated fatty acid index in animals with 4-week CCl 4 insult as compared with normal animals. Conclusion: The hepatic lipid changes in CCl 4-induced experimental fibrosis model were documented in vivo and longitudinally using 1H MRS at 7 Tesla. The experimental findings suggested that total saturated fatty acid increase contributed mainly to the total lipid increase in animals with CCl 4 insult. This study also demonstrated the potential value of high field MRS to resolve lipid composition and alterations in liver fibrosis. © 2011 AUR.en_HK
dc.languageengen_US
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/araden_HK
dc.relation.ispartofAcademic Radiologyen_HK
dc.subjectCarbon tetrachloride (CCl 4)en_HK
dc.subjectLipiden_HK
dc.subjectLiver fibrosisen_HK
dc.subjectProton magnetic resonance spectroscopy ( 1H MRS)en_HK
dc.subjectSaturated fatty aciden_HK
dc.subjectUnsaturated fatty aciden_HK
dc.subject.meshDisease Models, Animal-
dc.subject.meshLipids - analysis-
dc.subject.meshLiver Cirrhosis - diagnosis - metabolism-
dc.subject.meshMagnetic Resonance Spectroscopy - methods-
dc.subject.meshProtons - diagnostic use-
dc.titleIn Vivo Lipid Profiling Using Proton Magnetic Resonance Spectroscopy in an Experimental Liver Fibrosis Modelen_HK
dc.typeArticleen_HK
dc.identifier.emailMan, K: kwanman@hkucc.hku.hken_HK
dc.identifier.emailWu, EX: ewu1@hkucc.hku.hken_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityWu, EX=rp00193en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.acra.2010.10.012en_HK
dc.identifier.pmid21167757-
dc.identifier.scopuseid_2-s2.0-79551562585en_HK
dc.identifier.hkuros188086en_US
dc.identifier.hkuros206801-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79551562585&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume18en_HK
dc.identifier.issue3en_HK
dc.identifier.spage377en_HK
dc.identifier.epage383en_HK
dc.identifier.isiWOS:000287620700016-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridCheung, JS=16174280400en_HK
dc.identifier.scopusauthoridFan, SJ=36514618100en_HK
dc.identifier.scopusauthoridGao, DS=36664395800en_HK
dc.identifier.scopusauthoridChow, AM=16174234200en_HK
dc.identifier.scopusauthoridYang, J=23391308100en_HK
dc.identifier.scopusauthoridMan, K=7101754072en_HK
dc.identifier.scopusauthoridWu, EX=7202128034en_HK
dc.identifier.citeulike8609146-

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