Article: In Vivo Lipid Profiling Using Proton Magnetic Resonance Spectroscopy in an Experimental Liver Fibrosis Model
| Title | In Vivo Lipid Profiling Using Proton Magnetic Resonance Spectroscopy in an Experimental Liver Fibrosis Model | ||||
|---|---|---|---|---|---|
| Authors | Cheung, JS1 2 Fan, SJ2 Gao, DS2 Chow, AM2 Yang, J2 3 Man, K2 Wu, EX2 | ||||
| Keywords | Carbon tetrachloride (CCl 4) Lipid Liver fibrosis Proton magnetic resonance spectroscopy ( 1H MRS) Saturated fatty acid Unsaturated fatty acid | ||||
| Issue Date | 2011 | ||||
| Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/arad | ||||
| Citation | Academic Radiology, 2011, v. 18 n. 3, p. 377-383 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.acra.2010.10.012 | ||||
| Abstract | Rationale and Objectives: The aim of this study was to characterize early hepatic lipid changes in an experimental model of liver fibrosis using proton ( 1H) magnetic resonance spectroscopy (MRS) at high magnetic field in vivo. Materials and Methods: Liver fibrosis was induced in 12 Sprague-Dawley rats by twice-weekly carbon tetrachloride (CCl 4) administration up to 4 weeks. Eight normal rats were used as controls. Single-voxel 1H MRS experiments were performed at 7 Tesla to measure signal integrals of various lipid peaks including -CH 3, (-CH 2-) n, -CH 2-C=C-CH 2-, =C-CH 2-C= and -CH=CH- at 0.9, 1.3, 2.0, 2.8, and 5.3 ppm, respectively, and peak from choline-containing compounds (CCC) at 3.2 ppm. Total lipid, total saturated fatty acid, total unsaturated fatty acid, total unsaturated bond, polyunsaturated bond, and CCC indices were quantified. Results: Significant increases (P<.01) in total lipid and total saturated fatty acid indices were found in animals with CCl 4-induced fibrosis as compared with normal animals. In addition, total unsaturated bond and polyunsaturated bond indices of animals at 4 weeks after CCl 4 insult were significantly higher than (P<.01 and P<.05, respectively) those of normal animals and animals at 2 weeks following insult; whereas there was only significant increase (P<.01) in total unsaturated fatty acid index in animals with 4-week CCl 4 insult as compared with normal animals. Conclusion: The hepatic lipid changes in CCl 4-induced experimental fibrosis model were documented in vivo and longitudinally using 1H MRS at 7 Tesla. The experimental findings suggested that total saturated fatty acid increase contributed mainly to the total lipid increase in animals with CCl 4 insult. This study also demonstrated the potential value of high field MRS to resolve lipid composition and alterations in liver fibrosis. © 2011 AUR. | ||||
| ISSN | 1076-6332 2011 Impact Factor: 1.692 2011 SCImago Journal Rankings: 0.141 | ||||
| DOI | http://dx.doi.org/10.1016/j.acra.2010.10.012 | ||||
| ISI Accession Number ID | WOS:000287620700016
Funding Information: From the Laboratory of Biomedical Imaging and Signal Processing (J.S.C., S.J.F., D.S.G., A.M.C., J.Y., E.X.W.), Departments of Electrical and Electronic Engineering (J.S.C., S.J.F., D.S.G., A.M.C., J.Y., E.X.W.), Surgery (KM.), and Anatomy (E.X.W.), The University of Hong Kong, Pokfulam, Hong Kong SAR, China; Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA (J.S.C); Department of Diagnostic Radiology of the First Affiliated Hospital, School of Medicine of Xi'an Jiaotong University, Xi'an, Shannxi Province, China (J.Y.). Received September 12, 2010; accepted October 29, 2010. Supported by the Hong Kong Grant Council (GRF HKU7808/09M). Address correspondence to: E.X.W. e-mail: ewu@eee.hku.hk | ||||
| References | References in Scopus |
| dc.contributor.author | Cheung, JS | ||||
|---|---|---|---|---|---|
| dc.contributor.author | Fan, SJ | ||||
| dc.contributor.author | Gao, DS | ||||
| dc.contributor.author | Chow, AM | ||||
| dc.contributor.author | Yang, J | ||||
| dc.contributor.author | Man, K | ||||
| dc.contributor.author | Wu, EX | ||||
| dc.date.accessioned | 2011-07-27T01:36:54Z | ||||
| dc.date.available | 2011-07-27T01:36:54Z | ||||
| dc.date.issued | 2011 | ||||
| dc.description.abstract | Rationale and Objectives: The aim of this study was to characterize early hepatic lipid changes in an experimental model of liver fibrosis using proton ( 1H) magnetic resonance spectroscopy (MRS) at high magnetic field in vivo. Materials and Methods: Liver fibrosis was induced in 12 Sprague-Dawley rats by twice-weekly carbon tetrachloride (CCl 4) administration up to 4 weeks. Eight normal rats were used as controls. Single-voxel 1H MRS experiments were performed at 7 Tesla to measure signal integrals of various lipid peaks including -CH 3, (-CH 2-) n, -CH 2-C=C-CH 2-, =C-CH 2-C= and -CH=CH- at 0.9, 1.3, 2.0, 2.8, and 5.3 ppm, respectively, and peak from choline-containing compounds (CCC) at 3.2 ppm. Total lipid, total saturated fatty acid, total unsaturated fatty acid, total unsaturated bond, polyunsaturated bond, and CCC indices were quantified. Results: Significant increases (P<.01) in total lipid and total saturated fatty acid indices were found in animals with CCl 4-induced fibrosis as compared with normal animals. In addition, total unsaturated bond and polyunsaturated bond indices of animals at 4 weeks after CCl 4 insult were significantly higher than (P<.01 and P<.05, respectively) those of normal animals and animals at 2 weeks following insult; whereas there was only significant increase (P<.01) in total unsaturated fatty acid index in animals with 4-week CCl 4 insult as compared with normal animals. Conclusion: The hepatic lipid changes in CCl 4-induced experimental fibrosis model were documented in vivo and longitudinally using 1H MRS at 7 Tesla. The experimental findings suggested that total saturated fatty acid increase contributed mainly to the total lipid increase in animals with CCl 4 insult. This study also demonstrated the potential value of high field MRS to resolve lipid composition and alterations in liver fibrosis. © 2011 AUR. | ||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||
| dc.identifier.citation | Academic Radiology, 2011, v. 18 n. 3, p. 377-383 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.acra.2010.10.012 | ||||
| dc.identifier.citeulike | 8609146 | ||||
| dc.identifier.doi | http://dx.doi.org/10.1016/j.acra.2010.10.012 | ||||
| dc.identifier.epage | 383 | ||||
| dc.identifier.hkuros | 188086 | ||||
| dc.identifier.hkuros | 206801 | ||||
| dc.identifier.isi | WOS:000287620700016
Funding Information: From the Laboratory of Biomedical Imaging and Signal Processing (J.S.C., S.J.F., D.S.G., A.M.C., J.Y., E.X.W.), Departments of Electrical and Electronic Engineering (J.S.C., S.J.F., D.S.G., A.M.C., J.Y., E.X.W.), Surgery (KM.), and Anatomy (E.X.W.), The University of Hong Kong, Pokfulam, Hong Kong SAR, China; Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA (J.S.C); Department of Diagnostic Radiology of the First Affiliated Hospital, School of Medicine of Xi'an Jiaotong University, Xi'an, Shannxi Province, China (J.Y.). Received September 12, 2010; accepted October 29, 2010. Supported by the Hong Kong Grant Council (GRF HKU7808/09M). Address correspondence to: E.X.W. e-mail: ewu@eee.hku.hk | ||||
| dc.identifier.issn | 1076-6332 2011 Impact Factor: 1.692 2011 SCImago Journal Rankings: 0.141 | ||||
| dc.identifier.issue | 3 | ||||
| dc.identifier.pmid | 21167757 | ||||
| dc.identifier.scopus | eid_2-s2.0-79551562585 | ||||
| dc.identifier.spage | 377 | ||||
| dc.identifier.uri | http://hdl.handle.net/10722/135548 | ||||
| dc.identifier.volume | 18 | ||||
| dc.language | eng | ||||
| dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/arad | ||||
| dc.publisher.place | Netherlands | ||||
| dc.relation.ispartof | Academic Radiology | ||||
| dc.relation.references | References in Scopus | ||||
| dc.subject.mesh | Disease Models, Animal | ||||
| dc.subject.mesh | Lipids - analysis | ||||
| dc.subject.mesh | Liver Cirrhosis - diagnosis - metabolism | ||||
| dc.subject.mesh | Magnetic Resonance Spectroscopy - methods | ||||
| dc.subject.mesh | Protons - diagnostic use | ||||
| dc.subject | Carbon tetrachloride (CCl 4) | ||||
| dc.subject | Lipid | ||||
| dc.subject | Liver fibrosis | ||||
| dc.subject | Proton magnetic resonance spectroscopy ( 1H MRS) | ||||
| dc.subject | Saturated fatty acid | ||||
| dc.subject | Unsaturated fatty acid | ||||
| dc.title | In Vivo Lipid Profiling Using Proton Magnetic Resonance Spectroscopy in an Experimental Liver Fibrosis Model | ||||
| dc.type | Article |
Author Affiliations
- Massachusetts General Hospital and Harvard Medical School
- The University of Hong Kong
- Xi'an Jiaotong University