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Article: Identification of liver proteins and their roles associated with carbon tetrachloride-induced hepatotoxicity

TitleIdentification of liver proteins and their roles associated with carbon tetrachloride-induced hepatotoxicity
Authors
Keywordscarbon tetrachloride
hepatotoxicity
liver
proteomics
reactive oxygen species
Issue Date2011
PublisherSage Publications Ltd. The Journal's web site is located at http://het.sagepub.com
Citation
Human And Experimental Toxicology, 2011, v. 30 n. 9, p. 1369-1381 How to Cite?
AbstractCarbon tetrachloride (CCl4) is a common hepatotoxin used in experimental models to elicit liver injury. To identify the proteins involved in CCl4-induced hepatotoxicity, two-dimensional gel electrophoresis was employed followed by mass spectrometry - mass spectrometry (MS/MS) to study the differentially expressed proteins during CCl4 exposure in the Fischer 344 rat liver proteome for 5 weeks. Ten spots with notable changes between the Control and CCl4 groups were successfully identified. Among them, four proteins with significant up-regulation, namely calcium-binding protein 1, protein disulfide isomerase, mitochondrial aldehyde dehydrogenase precursor, and, glutathione-S-transferase mu1 and six proteins with significant down-regulation, namely catechol-O-methyltransferase, hemoglobin-alpha-2-chain, hemopexin precursor, methionine sulfoxide reductase A, catalase and carbonic anhydrase 3, were identified. The data indicates that CCl4 causes hepatotoxicity by depleting oxygen radical scavengers in the hepatocytes. In this rat model, we profiled hepatic proteome alterations in response to CCl4 intoxication. The findings should facilitate understanding of the mechanism of CCl4-induced liver injury. © 2011 SAGE Publications.
Persistent Identifierhttp://hdl.handle.net/10722/135546
ISSN
2015 Impact Factor: 1.604
2015 SCImago Journal Rankings: 0.569
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong
Funding Information:

This work was supported by the grants of the Liver Strategic Research Grant by The University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorWong, LLYen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorMan, Ken_HK
dc.contributor.authorSit, WHen_HK
dc.contributor.authorJiang, PPen_HK
dc.contributor.authorJor, IWYen_HK
dc.contributor.authorLee, CYKen_HK
dc.contributor.authorLing, WLen_HK
dc.contributor.authorTam, KTen_HK
dc.contributor.authorWan, JMFen_HK
dc.date.accessioned2011-07-27T01:36:52Z-
dc.date.available2011-07-27T01:36:52Z-
dc.date.issued2011en_HK
dc.identifier.citationHuman And Experimental Toxicology, 2011, v. 30 n. 9, p. 1369-1381en_HK
dc.identifier.issn0960-3271en_HK
dc.identifier.urihttp://hdl.handle.net/10722/135546-
dc.description.abstractCarbon tetrachloride (CCl4) is a common hepatotoxin used in experimental models to elicit liver injury. To identify the proteins involved in CCl4-induced hepatotoxicity, two-dimensional gel electrophoresis was employed followed by mass spectrometry - mass spectrometry (MS/MS) to study the differentially expressed proteins during CCl4 exposure in the Fischer 344 rat liver proteome for 5 weeks. Ten spots with notable changes between the Control and CCl4 groups were successfully identified. Among them, four proteins with significant up-regulation, namely calcium-binding protein 1, protein disulfide isomerase, mitochondrial aldehyde dehydrogenase precursor, and, glutathione-S-transferase mu1 and six proteins with significant down-regulation, namely catechol-O-methyltransferase, hemoglobin-alpha-2-chain, hemopexin precursor, methionine sulfoxide reductase A, catalase and carbonic anhydrase 3, were identified. The data indicates that CCl4 causes hepatotoxicity by depleting oxygen radical scavengers in the hepatocytes. In this rat model, we profiled hepatic proteome alterations in response to CCl4 intoxication. The findings should facilitate understanding of the mechanism of CCl4-induced liver injury. © 2011 SAGE Publications.en_HK
dc.languageengen_US
dc.publisherSage Publications Ltd. The Journal's web site is located at http://het.sagepub.comen_HK
dc.relation.ispartofHuman and Experimental Toxicologyen_HK
dc.rightsHuman & Experimental Toxicology. Copyright © Sage Publications Ltd.-
dc.subjectcarbon tetrachlorideen_HK
dc.subjecthepatotoxicityen_HK
dc.subjectliveren_HK
dc.subjectproteomicsen_HK
dc.subjectreactive oxygen speciesen_HK
dc.titleIdentification of liver proteins and their roles associated with carbon tetrachloride-induced hepatotoxicityen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0960-3271&volume=30&issue=9&spage=1369&epage=1381&date=2010&atitle=Identification+of+liver+proteins+and+their+roles+associated+with+carbon+tetrachloride-induced+hepatotoxicity-
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.emailMan, K: kwanman@hku.hken_HK
dc.identifier.emailWan, JMF: jmfwan@hku.hken_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityWan, JMF=rp00798en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1177/0960327110391388en_HK
dc.identifier.pmid21138988-
dc.identifier.scopuseid_2-s2.0-80052521476en_HK
dc.identifier.hkuros187690en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80052521476&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume30en_HK
dc.identifier.issue9en_HK
dc.identifier.spage1369en_HK
dc.identifier.epage1381en_HK
dc.identifier.isiWOS:000294473300025-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWong, LLY=36128985900en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK
dc.identifier.scopusauthoridMan, K=7101754072en_HK
dc.identifier.scopusauthoridSit, WH=8528923000en_HK
dc.identifier.scopusauthoridJiang, PP=36147603700en_HK
dc.identifier.scopusauthoridJor, IWY=35731710200en_HK
dc.identifier.scopusauthoridLee, CYK=38162995600en_HK
dc.identifier.scopusauthoridLing, WL=51763910900en_HK
dc.identifier.scopusauthoridTam, KT=51764433700en_HK
dc.identifier.scopusauthoridWan, JMF=8930305000en_HK

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