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Article: The decrement in circulating endothelial progenitor cells (EPCs) in type 2 diabetes is independent of the severity of the hypoadiponectemia

TitleThe decrement in circulating endothelial progenitor cells (EPCs) in type 2 diabetes is independent of the severity of the hypoadiponectemia
Authors
KeywordsAdiponectin
Endothelial progenitor cells
Type 2 diabetes mellitus
Issue Date2011
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/10009394
Citation
Diabetes/Metabolism Research And Reviews, 2011, v. 27 n. 2, p. 185-194 How to Cite?
AbstractBackground: Type 2 diabetes mellitus (DM) is associated with a decreased level of circulating endothelial progenitor cells (EPCs) and adiponectin. Experimental studies suggest a potential link between hypoadiponectinaemia and the depletion of the EPC level. This study investigated the relationships between adiponectin level and EPC in patients with type 2 DM. Methods: A total of 95 type 2 DM patients (58.5 ± 8.8 years, 42 men) and 95 age- and sex-matched healthy controls were recruited. Circulating EPC levels were determined by flow cytometry using CD133 +, CD34 +, CD133 +/KDR + and CD34 +/KDR + as surface markers. Plasma adiponectin levels were measured by enzyme-linked immunosorbent assay. EPC function was studied by in vitro tube formation and migration assay. Results: The levels of CD133 + (p < 0.001) and CD133 +/KDR + (p < 0.001) EPCs were independently associated with the presence of type 2 DM. The levels of CD34 + (p = 0.004) and CD34 +/KDR + (p = 0.013) EPCs were independently associated with haemoglobin A 1c. Nevertheless, there was no relationship between the number of EPCs and adiponectin level. Tube formation assay showed impaired pro-angiogenic function of EPC in DM patients compared with controls (p = 0.007). Interestingly, adiponectin supplementation (5 μg/mL) increased tube formation by 17.6% in EPCs from DM patients (p = 0.002). It also significantly enhanced cell migration by 35.9% in EPCs from DM patients (p = 0.01). Conclusions: We detected no relationship between the reduction in the level of EPC and in the level of total adiponectin in blood from patients with type 2 diabetes. EPC from patients with diabetes were stimulated when exposed to adiponectin in the test tube, findings that warrant further study. Copyright © 2011 John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/135213
ISSN
2012 Impact Factor: 2.968
2015 SCImago Journal Rankings: 1.617
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grant CouncilHKU 8/CRF/09
University of Hong Kong200907176063
Sun Chieh Yeh Heart Foundation
Funding Information:

This study was supported by Collaborative Research Fund of Hong Kong Research Grant Council (HKU 8/CRF/09), Outstanding Researcher Award of the University of Hong Kong (HF Tse), CRCG Small Project Funding of University of Hong Kong (Project No. 200907176063), and Sun Chieh Yeh Heart Foundation.

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DC FieldValueLanguage
dc.contributor.authorLi, Men_HK
dc.contributor.authorHo, JCYen_HK
dc.contributor.authorLai, KWHen_HK
dc.contributor.authorAu, KKWen_HK
dc.contributor.authorXu, Aen_HK
dc.contributor.authorCheung, BMYen_HK
dc.contributor.authorLam, KSLen_HK
dc.contributor.authorTse, HFen_HK
dc.date.accessioned2011-07-27T01:30:03Z-
dc.date.available2011-07-27T01:30:03Z-
dc.date.issued2011en_HK
dc.identifier.citationDiabetes/Metabolism Research And Reviews, 2011, v. 27 n. 2, p. 185-194en_HK
dc.identifier.issn1520-7552en_HK
dc.identifier.urihttp://hdl.handle.net/10722/135213-
dc.description.abstractBackground: Type 2 diabetes mellitus (DM) is associated with a decreased level of circulating endothelial progenitor cells (EPCs) and adiponectin. Experimental studies suggest a potential link between hypoadiponectinaemia and the depletion of the EPC level. This study investigated the relationships between adiponectin level and EPC in patients with type 2 DM. Methods: A total of 95 type 2 DM patients (58.5 ± 8.8 years, 42 men) and 95 age- and sex-matched healthy controls were recruited. Circulating EPC levels were determined by flow cytometry using CD133 +, CD34 +, CD133 +/KDR + and CD34 +/KDR + as surface markers. Plasma adiponectin levels were measured by enzyme-linked immunosorbent assay. EPC function was studied by in vitro tube formation and migration assay. Results: The levels of CD133 + (p < 0.001) and CD133 +/KDR + (p < 0.001) EPCs were independently associated with the presence of type 2 DM. The levels of CD34 + (p = 0.004) and CD34 +/KDR + (p = 0.013) EPCs were independently associated with haemoglobin A 1c. Nevertheless, there was no relationship between the number of EPCs and adiponectin level. Tube formation assay showed impaired pro-angiogenic function of EPC in DM patients compared with controls (p = 0.007). Interestingly, adiponectin supplementation (5 μg/mL) increased tube formation by 17.6% in EPCs from DM patients (p = 0.002). It also significantly enhanced cell migration by 35.9% in EPCs from DM patients (p = 0.01). Conclusions: We detected no relationship between the reduction in the level of EPC and in the level of total adiponectin in blood from patients with type 2 diabetes. EPC from patients with diabetes were stimulated when exposed to adiponectin in the test tube, findings that warrant further study. Copyright © 2011 John Wiley & Sons, Ltd.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/10009394en_HK
dc.relation.ispartofDiabetes/Metabolism Research and Reviewsen_HK
dc.rightsDiabetes - Metabolism: Research and Reviews. Copyright © John Wiley & Sons Ltd.-
dc.subjectAdiponectinen_HK
dc.subjectEndothelial progenitor cellsen_HK
dc.subjectType 2 diabetes mellitusen_HK
dc.subject.meshAdiponectin - blood-
dc.subject.meshCells, Cultured-
dc.subject.meshDiabetes Mellitus, Type 2 - blood - pathology-
dc.subject.meshEndothelium, Vascular - cytology - metabolism-
dc.subject.meshStem Cells - metabolism-
dc.titleThe decrement in circulating endothelial progenitor cells (EPCs) in type 2 diabetes is independent of the severity of the hypoadiponectemiaen_HK
dc.typeArticleen_HK
dc.identifier.emailXu, A:amxu@hkucc.hku.hken_HK
dc.identifier.emailCheung, BMY:mycheung@hku.hken_HK
dc.identifier.emailLam, KSL:ksllam@hku.hken_HK
dc.identifier.emailTse, HF:hftse@hkucc.hku.hken_HK
dc.identifier.authorityXu, A=rp00485en_HK
dc.identifier.authorityCheung, BMY=rp01321en_HK
dc.identifier.authorityLam, KSL=rp00343en_HK
dc.identifier.authorityTse, HF=rp00428en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/dmrr.1159en_HK
dc.identifier.pmid21294240-
dc.identifier.scopuseid_2-s2.0-79551584062en_HK
dc.identifier.hkuros187099en_US
dc.identifier.hkuros174101-
dc.identifier.hkuros183463-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-79551584062&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume27en_HK
dc.identifier.issue2en_HK
dc.identifier.spage185en_HK
dc.identifier.epage194en_HK
dc.identifier.isiWOS:000287155500005-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectPluripotent Human Stem Cell Platform for Tissue Regeneration and Drug Screening for Cardiovascular Diseases-
dc.identifier.scopusauthoridLi, M=36465410000en_HK
dc.identifier.scopusauthoridHo, JCY=7402650173en_HK
dc.identifier.scopusauthoridLai, KWH=7402135541en_HK
dc.identifier.scopusauthoridAu, KKW=38662193900en_HK
dc.identifier.scopusauthoridXu, A=7202655409en_HK
dc.identifier.scopusauthoridCheung, BMY=7103294806en_HK
dc.identifier.scopusauthoridLam, KSL=8082870600en_HK
dc.identifier.scopusauthoridTse, HF=7006070805en_HK

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