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Article: Inhibition of LZIP-mediated transcription through direct interaction with a novel host cell factor-like protein

TitleInhibition of LZIP-mediated transcription through direct interaction with a novel host cell factor-like protein
Authors
Issue Date2001
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 2001, v. 276 n. 31, p. 28933-28938 How to Cite?
AbstractHost cell factor 1 (HCF-1) is a cellular transcriptional coactivator which coordinates the assembly of enhancer complex through direct interactions with viral and cellular trans-activators such as VP16, Oct-1, LZIP, and GA-binding protein. These interactions are mediated by the β-propeller domain comprising the first 380 residues of HCF-1 with six kelch repeats. Here we describe the identification and characterization of a novel HCF-like kelch repeat protein, designated HCLP-1. HCLP-1 is a ubiquitously expressed nuclear protein which is composed almost entirely of a six-bladed β-propeller. HCLP-1 selectively interacts with LZIP but not with VP16. The physical interaction between HCLP-1 and LZIP leads to the repression of the LZIP-dependent transcription. The HCLP-1-binding domain of LZIP maps to residues 109-315, which contain the bZIP DNA-binding motif. Electrophoretic mobility shift assay demonstrates that HCLP-1 indeed interferes with the binding of LZIP to its DNA target. Thus, HCLP-1 serves a transcriptional co-repressor function mediated through its inhibitory interaction with the LZIP transcription factor. Our findings suggest a new mechanism for transcriptional regulation by HCF-like proteins.
Persistent Identifierhttp://hdl.handle.net/10722/134152
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhou, HJen_HK
dc.contributor.authorWong, CMen_HK
dc.contributor.authorChen, JHen_HK
dc.contributor.authorQiang, BQen_HK
dc.contributor.authorYuan, JGen_HK
dc.contributor.authorJin, DYen_HK
dc.date.accessioned2011-06-13T07:20:15Z-
dc.date.available2011-06-13T07:20:15Z-
dc.date.issued2001en_HK
dc.identifier.citationJournal Of Biological Chemistry, 2001, v. 276 n. 31, p. 28933-28938en_HK
dc.identifier.issn0021-9258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/134152-
dc.description.abstractHost cell factor 1 (HCF-1) is a cellular transcriptional coactivator which coordinates the assembly of enhancer complex through direct interactions with viral and cellular trans-activators such as VP16, Oct-1, LZIP, and GA-binding protein. These interactions are mediated by the β-propeller domain comprising the first 380 residues of HCF-1 with six kelch repeats. Here we describe the identification and characterization of a novel HCF-like kelch repeat protein, designated HCLP-1. HCLP-1 is a ubiquitously expressed nuclear protein which is composed almost entirely of a six-bladed β-propeller. HCLP-1 selectively interacts with LZIP but not with VP16. The physical interaction between HCLP-1 and LZIP leads to the repression of the LZIP-dependent transcription. The HCLP-1-binding domain of LZIP maps to residues 109-315, which contain the bZIP DNA-binding motif. Electrophoretic mobility shift assay demonstrates that HCLP-1 indeed interferes with the binding of LZIP to its DNA target. Thus, HCLP-1 serves a transcriptional co-repressor function mediated through its inhibitory interaction with the LZIP transcription factor. Our findings suggest a new mechanism for transcriptional regulation by HCF-like proteins.en_HK
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_HK
dc.relation.ispartofJournal of Biological Chemistryen_HK
dc.subject.meshCyclic AMP Response Element-Binding Protein-
dc.subject.meshGene Expression Regulation-
dc.subject.meshProteins - chemistry - metabolism-
dc.subject.meshTranscription Factors - chemistry - metabolism-
dc.subject.meshTranscription, Genetic-
dc.titleInhibition of LZIP-mediated transcription through direct interaction with a novel host cell factor-like proteinen_HK
dc.typeArticleen_HK
dc.identifier.emailWong, CM:wispwong@hkucc.hku.hken_HK
dc.identifier.emailJin, DY:dyjin@hkucc.hku.hken_HK
dc.identifier.authorityWong, CM=rp01489en_HK
dc.identifier.authorityJin, DY=rp00452en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1074/jbc.M103893200en_HK
dc.identifier.scopuseid_2-s2.0-0035800752en_HK
dc.identifier.hkuros69703-
dc.identifier.hkuros60400-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0035800752&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume276en_HK
dc.identifier.issue31en_HK
dc.identifier.spage28933en_HK
dc.identifier.epage28938en_HK
dc.identifier.isiWOS:000170346000041-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhou, HJ=38062651800en_HK
dc.identifier.scopusauthoridWong, CM=18134632400en_HK
dc.identifier.scopusauthoridChen, JH=7501887992en_HK
dc.identifier.scopusauthoridQiang, BQ=7005510394en_HK
dc.identifier.scopusauthoridYuan, JG=7403401529en_HK
dc.identifier.scopusauthoridJin, DY=7201973614en_HK

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