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Article: Cooperation of yeast peroxiredoxins Tsa1p and Tsa2p in the cellular defense against oxidative and nitrosative stress

TitleCooperation of yeast peroxiredoxins Tsa1p and Tsa2p in the cellular defense against oxidative and nitrosative stress
Authors
KeywordsAnimalia
Saccharomyces
Saccharomyces Cerevisiae
Issue Date2002
PublisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/
Citation
Journal Of Biological Chemistry, 2002, v. 277 n. 7, p. 5385-5394 How to Cite?
AbstractPeroxiredoxins are a family of antioxidant enzymes conserved from bacteria to humans. In Saccharomyces cerevisiae, there exist five peroxiredoxins, among which Tsa2p shares striking homology with the well described Tsa1p but has not been extensively studied. Here we report on the functional characterization of yeast tsa2Δ mutants and the comparison of TSA1 with TSA2. The tsa2Δ and tsa1Δ tsa2Δ cells grew normally under aerobic conditions. However, the tsa1Δ tsa2Δ mutant yeast was more susceptible to oxidants than either tsa1Δ or tsa2Δ cells. Notably, the tsa1Δ tsa2Δ yeast was also hypersensitive to peroxynitrite and sodium nitroprusside. This phenotype was rescued by the expression of either the TSA1 or TSA2 gene. The demonstration of a peroxynitrite reductase activity of Tsa2p in vitro points to a pivotal role for peroxiredoxins in the protection against nitrosative stress. In yeast cells, Tsa1p and Tsa2p exhibited comparable antioxidant activity. While the basal expression level of TSA1 was significantly higher than that of TSA2, the transcription of TSA2 was stimulated more potently by various oxidants. In addition, TSA2 was activated in tsa1Δ cells in a Yap1p-dependent manner. Taken together, our findings implicate the cooperation of Tsa1p and Tsa2p in the cellular defense against reactive oxygen and nitrogen species.
Persistent Identifierhttp://hdl.handle.net/10722/134151
ISSN
2015 Impact Factor: 4.258
2015 SCImago Journal Rankings: 3.151
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, CMen_HK
dc.contributor.authorZhou, Yen_HK
dc.contributor.authorNg, RWMen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorJin, DYen_HK
dc.date.accessioned2011-06-13T07:20:15Z-
dc.date.available2011-06-13T07:20:15Z-
dc.date.issued2002en_HK
dc.identifier.citationJournal Of Biological Chemistry, 2002, v. 277 n. 7, p. 5385-5394en_HK
dc.identifier.issn0021-9258en_HK
dc.identifier.urihttp://hdl.handle.net/10722/134151-
dc.description.abstractPeroxiredoxins are a family of antioxidant enzymes conserved from bacteria to humans. In Saccharomyces cerevisiae, there exist five peroxiredoxins, among which Tsa2p shares striking homology with the well described Tsa1p but has not been extensively studied. Here we report on the functional characterization of yeast tsa2Δ mutants and the comparison of TSA1 with TSA2. The tsa2Δ and tsa1Δ tsa2Δ cells grew normally under aerobic conditions. However, the tsa1Δ tsa2Δ mutant yeast was more susceptible to oxidants than either tsa1Δ or tsa2Δ cells. Notably, the tsa1Δ tsa2Δ yeast was also hypersensitive to peroxynitrite and sodium nitroprusside. This phenotype was rescued by the expression of either the TSA1 or TSA2 gene. The demonstration of a peroxynitrite reductase activity of Tsa2p in vitro points to a pivotal role for peroxiredoxins in the protection against nitrosative stress. In yeast cells, Tsa1p and Tsa2p exhibited comparable antioxidant activity. While the basal expression level of TSA1 was significantly higher than that of TSA2, the transcription of TSA2 was stimulated more potently by various oxidants. In addition, TSA2 was activated in tsa1Δ cells in a Yap1p-dependent manner. Taken together, our findings implicate the cooperation of Tsa1p and Tsa2p in the cellular defense against reactive oxygen and nitrogen species.en_HK
dc.languageengen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology, Inc. The Journal's web site is located at http://www.jbc.org/en_HK
dc.relation.ispartofJournal of Biological Chemistryen_HK
dc.subjectAnimaliaen_US
dc.subjectSaccharomycesen_US
dc.subjectSaccharomyces Cerevisiaeen_US
dc.subject.meshBlotting, Northernen_HK
dc.subject.meshBlotting, Southernen_HK
dc.subject.meshDose-Response Relationship, Drugen_HK
dc.subject.meshEvolution, Molecularen_HK
dc.subject.meshFlow Cytometryen_HK
dc.subject.meshHistidine - chemistryen_HK
dc.subject.meshLac Operonen_HK
dc.subject.meshMutationen_HK
dc.subject.meshNitrogen - metabolismen_HK
dc.subject.meshOxidative Stressen_HK
dc.subject.meshOxidoreductases - metabolismen_HK
dc.subject.meshOxygen - metabolismen_HK
dc.subject.meshPeroxidases - chemistry - metabolismen_HK
dc.subject.meshPeroxiredoxinsen_HK
dc.subject.meshPlasmids - metabolismen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshProtein Bindingen_HK
dc.subject.meshReactive Oxygen Speciesen_HK
dc.subject.meshRecombinant Fusion Proteins - metabolismen_HK
dc.subject.meshSaccharomyces cerevisiae - metabolismen_HK
dc.subject.meshTime Factorsen_HK
dc.subject.meshbeta-Galactosidase - metabolismen_HK
dc.titleCooperation of yeast peroxiredoxins Tsa1p and Tsa2p in the cellular defense against oxidative and nitrosative stressen_HK
dc.typeArticleen_HK
dc.identifier.emailWong, CM:wispwong@hkucc.hku.hken_HK
dc.identifier.emailJin, DY:dyjin@hkucc.hku.hken_HK
dc.identifier.authorityWong, CM=rp01489en_HK
dc.identifier.authorityJin, DY=rp00452en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1074/jbc.M106846200en_HK
dc.identifier.pmid11741925-
dc.identifier.scopuseid_2-s2.0-0037085384en_HK
dc.identifier.hkuros68769-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037085384&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume277en_HK
dc.identifier.issue7en_HK
dc.identifier.spage5385en_HK
dc.identifier.epage5394en_HK
dc.identifier.isiWOS:000173962900107-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWong, CM=18134632400en_HK
dc.identifier.scopusauthoridZhou, Y=7405366890en_HK
dc.identifier.scopusauthoridNg, RWM=7102153861en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridJin, DY=7201973614en_HK

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