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Article: Characterization of human and mouse peroxiredoxin IV: Evidence for inhibition by Prx-IV of epidermal growth factor- and p53-induced reactive oxygen species

TitleCharacterization of human and mouse peroxiredoxin IV: Evidence for inhibition by Prx-IV of epidermal growth factor- and p53-induced reactive oxygen species
Authors
KeywordsSpecies Index: Bacteria (Microorganisms)
Issue Date2000
PublisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/ars
Citation
Antioxidants And Redox Signaling, 2000, v. 2 n. 3, p. 507-518 How to Cite?
AbstractThe aim of this study was to identify and characterize human and mouse Prx-IV. We identified mouse peroxiredoxin IV (Prx-IV) by virtue of sequence homology to its human ortholog previously called AOE372. Mouse Prx-IV conserves an amino-terminal presequence coding for signal peptide. The amino acid sequences of mature mouse and human Prx-IV share 97.5% identity. Phylogenetic analysis demonstrates that Prx-IV is more closely related to Prx-I/-II/-III than to Prx-V/-VI. Previously, we mapped the mouse Prx-IV gene to chromosome X by analyzing two sets of multiloci genetic crosses. Here we performed further comparative analysis of mouse and human Prx-IV genomic loci. Consistent with the mouse results, human Prx-IV gene localized to chromosome Xp22.135-136, in close proximity to SAT and DXS7178. A bacterial artificial chromosome (BAC) clone containing the complete human Prx-IV locus was identified. The size of 7 exons and the sequences of the splice junctions were confirmed by PCR analysis. We conclude that mouse Prx-IV is abundantly expressed in many tissues. However, we could not detect Prx-IV in the conditioned media of NIH-3T3 and Jurkat cells. Mouse Prx-IV was specifically found in the nucleus-excluded region of cultured mouse cells. Intracellularly, overexpression of mouse Prx-IV prevented the production of reactive oxygen species induced by epidermal growth factor or p53. Taken together, mouse Prx-IV is likely a cytoplasmic or organellar peroxiredoxin involved in intracellular redox signaling.
Persistent Identifierhttp://hdl.handle.net/10722/132366
ISSN
2015 Impact Factor: 7.093
2015 SCImago Journal Rankings: 3.134
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, CMen_HK
dc.contributor.authorChun, ACSen_HK
dc.contributor.authorKok, KHen_HK
dc.contributor.authorZhou, Yen_HK
dc.contributor.authorFung, PCWen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorJeang, KTen_HK
dc.contributor.authorJin, DYen_HK
dc.date.accessioned2011-03-28T09:23:38Z-
dc.date.available2011-03-28T09:23:38Z-
dc.date.issued2000en_HK
dc.identifier.citationAntioxidants And Redox Signaling, 2000, v. 2 n. 3, p. 507-518en_HK
dc.identifier.issn1523-0864en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132366-
dc.description.abstractThe aim of this study was to identify and characterize human and mouse Prx-IV. We identified mouse peroxiredoxin IV (Prx-IV) by virtue of sequence homology to its human ortholog previously called AOE372. Mouse Prx-IV conserves an amino-terminal presequence coding for signal peptide. The amino acid sequences of mature mouse and human Prx-IV share 97.5% identity. Phylogenetic analysis demonstrates that Prx-IV is more closely related to Prx-I/-II/-III than to Prx-V/-VI. Previously, we mapped the mouse Prx-IV gene to chromosome X by analyzing two sets of multiloci genetic crosses. Here we performed further comparative analysis of mouse and human Prx-IV genomic loci. Consistent with the mouse results, human Prx-IV gene localized to chromosome Xp22.135-136, in close proximity to SAT and DXS7178. A bacterial artificial chromosome (BAC) clone containing the complete human Prx-IV locus was identified. The size of 7 exons and the sequences of the splice junctions were confirmed by PCR analysis. We conclude that mouse Prx-IV is abundantly expressed in many tissues. However, we could not detect Prx-IV in the conditioned media of NIH-3T3 and Jurkat cells. Mouse Prx-IV was specifically found in the nucleus-excluded region of cultured mouse cells. Intracellularly, overexpression of mouse Prx-IV prevented the production of reactive oxygen species induced by epidermal growth factor or p53. Taken together, mouse Prx-IV is likely a cytoplasmic or organellar peroxiredoxin involved in intracellular redox signaling.en_HK
dc.languageengen_US
dc.publisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/arsen_HK
dc.relation.ispartofAntioxidants and Redox Signalingen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThis is a copy of an article published in the [Antioxidants and Redox Signaling] © [2000] [copyright Mary Ann Liebert, Inc.]; [Antioxidants and Redox Signaling] is available online at: http://www.liebertonline.com.-
dc.subjectSpecies Index: Bacteria (Microorganisms)en_US
dc.subject.mesh3T3 Cellsen_HK
dc.subject.meshAmino Acid Sequenceen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshBlotting, Northernen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshChromosome Mappingen_HK
dc.subject.meshCloning, Molecularen_HK
dc.subject.meshCulture Media, Conditioned - metabolismen_HK
dc.subject.meshEpidermal Growth Factor - antagonists & inhibitorsen_HK
dc.subject.meshExonsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshJurkat Cellsen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMicroscopy, Confocalen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshOxidation-Reductionen_HK
dc.subject.meshPeroxidases - chemistry - genetics - physiologyen_HK
dc.subject.meshPeroxiredoxinsen_HK
dc.subject.meshPhylogenyen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshProtein Sorting Signalsen_HK
dc.subject.meshRNA Splicingen_HK
dc.subject.meshRNA, Messenger - metabolismen_HK
dc.subject.meshReactive Oxygen Speciesen_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.subject.meshSequence Homology, Amino Aciden_HK
dc.subject.meshTime Factorsen_HK
dc.subject.meshTissue Distributionen_HK
dc.subject.meshTumor Suppressor Protein p53 - antagonists & inhibitorsen_HK
dc.subject.meshX Chromosomeen_HK
dc.titleCharacterization of human and mouse peroxiredoxin IV: Evidence for inhibition by Prx-IV of epidermal growth factor- and p53-induced reactive oxygen speciesen_HK
dc.typeArticleen_HK
dc.identifier.emailWong, CM:wispwong@hkucc.hku.hken_HK
dc.identifier.emailKok, KH:khkok@hku.hken_HK
dc.identifier.emailJin, DY:dyjin@hkucc.hku.hken_HK
dc.identifier.authorityWong, CM=rp01489en_HK
dc.identifier.authorityKok, KH=rp01455en_HK
dc.identifier.authorityJin, DY=rp00452en_HK
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1089/15230860050192288-
dc.identifier.pmid11229364-
dc.identifier.scopuseid_2-s2.0-0033734877en_HK
dc.identifier.hkuros53964-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033734877&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume2en_HK
dc.identifier.issue3en_HK
dc.identifier.spage507en_HK
dc.identifier.epage518en_HK
dc.identifier.isiWOS:000207500800015-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWong, CM=18134632400en_HK
dc.identifier.scopusauthoridChun, ACS=7003650706en_HK
dc.identifier.scopusauthoridKok, KH=7006862631en_HK
dc.identifier.scopusauthoridZhou, Y=7405366890en_HK
dc.identifier.scopusauthoridFung, PCW=7101613315en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridJeang, KT=7004824803en_HK
dc.identifier.scopusauthoridJin, DY=7201973614en_HK

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