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Article: Identification and validation of the cellular targets of virus-encoded microRNAs.

TitleIdentification and validation of the cellular targets of virus-encoded microRNAs.
Authors
Issue Date2010
Citation
Methods In Molecular Biology (Clifton, N.J.), 2010, v. 667, p. 319-326 How to Cite?
AbstractSince the identification of the first virus-encoded microRNA (miRNA) in Epstein-Barr virus (EBV)-infected B cells in 2004, viral miRNAs have been found in different groups of herpesviruses. Viral miRNAs play an important role in regulating both viral and cellular gene expression. Identification and characterization of the cellular targets of viral miRNAs will not only advance our understanding of virus-cell interaction but might also reveal new strategies for the developments of antivirals. Our demonstration of the targeting of p53 upregulated modulator of apoptosis (PUMA) by an EBV-encoded viral miRNA provides one mechanism by which a viral miRNA facilitates viral replication by promoting cell survival. Using EBV miRNAs as an example, in this chapter, we detail the experimental procedures that can be used to identify and validate cellular targets of viral miRNAs.
Persistent Identifierhttp://hdl.handle.net/10722/132355
ISBN
ISSN
Series/Report no.Springer protocols.
Methods in molecular biology (Clifton, N.J.); v. 667.

 

DC FieldValueLanguage
dc.contributor.authorKok, KHen_HK
dc.contributor.authorLei, Ten_HK
dc.contributor.authorJin, DYen_HK
dc.date.accessioned2011-03-28T09:23:24Z-
dc.date.available2011-03-28T09:23:24Z-
dc.date.issued2010en_HK
dc.identifier.citationMethods In Molecular Biology (Clifton, N.J.), 2010, v. 667, p. 319-326en_HK
dc.identifier.isbn9781607618102-
dc.identifier.issn1940-6029en_HK
dc.identifier.urihttp://hdl.handle.net/10722/132355-
dc.description.abstractSince the identification of the first virus-encoded microRNA (miRNA) in Epstein-Barr virus (EBV)-infected B cells in 2004, viral miRNAs have been found in different groups of herpesviruses. Viral miRNAs play an important role in regulating both viral and cellular gene expression. Identification and characterization of the cellular targets of viral miRNAs will not only advance our understanding of virus-cell interaction but might also reveal new strategies for the developments of antivirals. Our demonstration of the targeting of p53 upregulated modulator of apoptosis (PUMA) by an EBV-encoded viral miRNA provides one mechanism by which a viral miRNA facilitates viral replication by promoting cell survival. Using EBV miRNAs as an example, in this chapter, we detail the experimental procedures that can be used to identify and validate cellular targets of viral miRNAs.en_HK
dc.languageengen_US
dc.relation.ispartofMethods in molecular biology (Clifton, N.J.)en_HK
dc.relation.ispartofseriesSpringer protocols.-
dc.relation.ispartofseriesMethods in molecular biology (Clifton, N.J.); v. 667.-
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshGene Expression Regulation, Viralen_HK
dc.subject.meshHerpesvirus 4, Human - genetics - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMicroRNAs - genetics - metabolismen_HK
dc.subject.meshRNA, Viral - genetics - metabolismen_HK
dc.titleIdentification and validation of the cellular targets of virus-encoded microRNAs.en_HK
dc.typeArticleen_HK
dc.identifier.emailKok, KH:khkok@hku.hken_HK
dc.identifier.emailJin, DY:dyjin@hkucc.hku.hken_HK
dc.identifier.authorityKok, KH=rp01455en_HK
dc.identifier.authorityJin, DY=rp00452en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid20827543-
dc.identifier.scopuseid_2-s2.0-79952109234en_HK
dc.identifier.hkuros175720-
dc.identifier.hkuros170454-
dc.identifier.volume667en_HK
dc.identifier.spage319en_HK
dc.identifier.epage326en_HK
dc.identifier.scopusauthoridKok, KH=7006862631en_HK
dc.identifier.scopusauthoridLei, T=37000963500en_HK
dc.identifier.scopusauthoridJin, DY=7201973614en_HK

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