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Conference Paper: Effects of reconstituted collagen matrix on fates of mouse embryonic stem cells before and after induction for chondrogenic differentiation

TitleEffects of reconstituted collagen matrix on fates of mouse embryonic stem cells before and after induction for chondrogenic differentiation
Authors
Issue Date2009
PublisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=263
Citation
The 7th Annual Meeting of the International Society for Stem Cell Research (ISSCR), Barcelona, Spain, 8-11 July 2009. In Tissue Engineering. Part A, 2009, v. 15 n. 10, p. 3071-3085 How to Cite?
AbstractEmbryonic stem (ES) cells are pluripotent cells with great potential in regenerative medicine. However, controlling their differentiation toward homogeneous lineages is challenging. In this study, we aim to investigate the effects of reconstituted 3D collagen matrix on the fates of mouse ES (mES) cells before and after induction for chondrogenic differentiation. Specifically, mES cells were encapsulated and cultured in 3D collagen microspheres and exposed to induction signals at different time points. Growth characteristics and differentiation status of mES cells were then evaluated. Collagen microspheres provided a suitable microenvironment supporting mES cell growth and maintaining their undifferentiated status for certain period of time. At later time points, the proportion of undifferentiated mES cells gradually decreased, accompanied by increasing proportions of mesenchymal progenitor cells. This suggests the inductive role of collagen matrix in differentiating mES cells toward mesenchymal lineages. Moreover, a lower initial collagen monomer concentration facilitated the differentiation of mES cells into chondrogenic lineages, while induction at a later time point associated with a more advanced stage of chondrogenic differentiation. This indicates that both the initial collagen concentration and the time to induce differentiation significantly affected the fates of mES cells. This study contributes to future development of ES cell-based therapies. © Copyright 2009, Mary Ann Liebert, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/129823
ISSN
2015 SCImago Journal Rankings: 1.500
ISI Accession Number ID
Funding AgencyGrant Number
AOSpineAOSBRC-07-06
Innovation and Technology Commission
Hong Kong GovernmentGHP=050=06
University Research Committee10206799
University's Strategic Research Theme on Development, Growth and Reproduction
Funding Information:

This work was supported by grants from AOSpine (AOSBRC-07-06); the Innovation and Technology Commission, the Hong Kong Government (GHP=050=06); University Research Committee (10206799); and University's Strategic Research Theme on Development, Growth and Reproduction. The authors thank Prof. Andras Nagy for useful discussion and kind provision of the mES cells (R1), Ms. Alice Lui for technical assistance on the flow cytometry analysis, and the Department of Pediatrics and Adolescent Medicine for providing the flow cytometer for analysis.

References

 

DC FieldValueLanguage
dc.contributor.authorYeung, CWen_HK
dc.contributor.authorCheah, Ken_HK
dc.contributor.authorChan, Den_HK
dc.contributor.authorChan, BPen_HK
dc.date.accessioned2010-12-23T08:42:29Z-
dc.date.available2010-12-23T08:42:29Z-
dc.date.issued2009en_HK
dc.identifier.citationThe 7th Annual Meeting of the International Society for Stem Cell Research (ISSCR), Barcelona, Spain, 8-11 July 2009. In Tissue Engineering. Part A, 2009, v. 15 n. 10, p. 3071-3085en_HK
dc.identifier.issn1937-3341en_HK
dc.identifier.urihttp://hdl.handle.net/10722/129823-
dc.description.abstractEmbryonic stem (ES) cells are pluripotent cells with great potential in regenerative medicine. However, controlling their differentiation toward homogeneous lineages is challenging. In this study, we aim to investigate the effects of reconstituted 3D collagen matrix on the fates of mouse ES (mES) cells before and after induction for chondrogenic differentiation. Specifically, mES cells were encapsulated and cultured in 3D collagen microspheres and exposed to induction signals at different time points. Growth characteristics and differentiation status of mES cells were then evaluated. Collagen microspheres provided a suitable microenvironment supporting mES cell growth and maintaining their undifferentiated status for certain period of time. At later time points, the proportion of undifferentiated mES cells gradually decreased, accompanied by increasing proportions of mesenchymal progenitor cells. This suggests the inductive role of collagen matrix in differentiating mES cells toward mesenchymal lineages. Moreover, a lower initial collagen monomer concentration facilitated the differentiation of mES cells into chondrogenic lineages, while induction at a later time point associated with a more advanced stage of chondrogenic differentiation. This indicates that both the initial collagen concentration and the time to induce differentiation significantly affected the fates of mES cells. This study contributes to future development of ES cell-based therapies. © Copyright 2009, Mary Ann Liebert, Inc.en_HK
dc.languageengen_US
dc.publisherMary Ann Liebert, Inc. Publishers. The Journal's web site is located at http://www.liebertpub.com/publication.aspx?pub_id=263en_HK
dc.relation.ispartofTissue Engineering. Part Aen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThis is a copy of an article published in the Tissue Engineering. Part A © 2009. Copyright Mary Ann Liebert, Inc. Tissue Engineering. Part A is available online at: http://www.liebertonline.com.-
dc.subject.meshAnimals-
dc.subject.meshCell Differentiation - drug effects - physiology-
dc.subject.meshCollagen - metabolism - pharmacology-
dc.subject.meshEmbryonic Stem Cells - cytology - drug effects-
dc.subject.meshExtracellular Matrix - metabolism-
dc.titleEffects of reconstituted collagen matrix on fates of mouse embryonic stem cells before and after induction for chondrogenic differentiationen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1937-3341&volume=15&issue=10&spage=3071&epage=3085&date=2009&atitle=Effects+of+reconstituted+collagen+matrix+on+fates+of+mouse+embryonic+stem+cells+before+and+after+induction+for+chondrogenic+differentiation-
dc.identifier.emailCheah, K:hrmbdkc@hku.hken_HK
dc.identifier.emailChan, D:chand@hkucc.hku.hken_HK
dc.identifier.emailChan, BP:bpchan@hkucc.hku.hken_HK
dc.identifier.authorityCheah, K=rp00342en_HK
dc.identifier.authorityChan, D=rp00540en_HK
dc.identifier.authorityChan, BP=rp00087en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1089/ten.tea.2008.0661en_HK
dc.identifier.pmid19331579-
dc.identifier.scopuseid_2-s2.0-73349139564en_HK
dc.identifier.hkuros177478en_US
dc.identifier.hkuros164807-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-73349139564&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume15en_HK
dc.identifier.issue10en_HK
dc.identifier.spage3071en_HK
dc.identifier.epage3085en_HK
dc.identifier.isiWOS:000270553200028-
dc.publisher.placeUnited Statesen_HK
dc.description.otherThe 7th Annual Meeting of the International Society for Stem Cell Research (ISSCR), Barcelona, Spain, 8-11 July 2009. In Tissue Engineering. Part A, 2009, v. 15 n. 10, p. 3071-3085-
dc.identifier.scopusauthoridYeung, CW=18635690500en_HK
dc.identifier.scopusauthoridCheah, K=35387746200en_HK
dc.identifier.scopusauthoridChan, D=7402216545en_HK
dc.identifier.scopusauthoridChan, BP=7201530390en_HK

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