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Article: P634A4 and TAp73 immunocytochemistry in liquid-based cervical cytologypotential biomarkers for diagnosis and progress prediction of cervical neoplasia

TitleP634A4 and TAp73 immunocytochemistry in liquid-based cervical cytologypotential biomarkers for diagnosis and progress prediction of cervical neoplasia
Authors
KeywordsLiquid-based cervical cytology
P63
P73
Issue Date2010
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/modpathol/
Citation
Modern Pathology, 2010, v. 23 n. 4, p. 559-566 How to Cite?
Abstract
P63 and p73 are two homologues of the important tumor suppressor gene p53. In this study, we investigated p63 and p73 expression by immunocytochemistry using antibodies for TAp73 and p634A4 isoforms in 91 high-grade and 107 low-grade squamous intraepithelial lesions, 212 atypical squamous cells of undetermined significance, 9 squamous cell carcinomas and 63 normal samples from an Asian screening population together with 47 hospital samples of carcinomas. There was significant correlation between the TAp73 and p634A4 indices (P0.0001). Significantly, higher TAp73 and p634A4 indices were found in high-grade lesions or carcinoma when compared with atypical squamous cells and low-grade lesions (P0.0001). Among atypical squamous cells, p634A4 indices of cases that subsequently progressed to low-grade (P0.031) or high-grade lesions (P0.006) were significantly higher than those that did not. For atypical squamous cells positive for high-risk human papillomavirus (HPV) as detected by Digene (61%), cases with high p634A4 index were still more likely to have subsequent high-grade lesions detected (P0.016). Among low-grade lesions, significantly higher TAp73 (P0.038) was found in cases that subsequently progressed to high-grade lesions. There was significant correlation between presence of high-risk HPV and p634A4 index (P0.01). In summary, p63 and p73 immunocytochemistry are potential good markers for detection of carcinoma and high-grade lesions in cervical cytology samples and for triage management of women with atypical squamous cells and low-grade lesions. © 2010 USCAP, Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/129524
ISSN
2013 Impact Factor: 6.364
ISI Accession Number ID
Funding AgencyGrant Number
Research Fund for the Control of Infectious Diseases/Health and Health Services Fund06060642
SK Yee Medical Foundation
Funding Information:

This study was supported by the Research Fund for the Control of Infectious Diseases/Health and Health Services Fund (Project Number: 06060642). The high-risk HPV Hybrid Capture Test II was supported by the SK Yee Medical Foundation.

References
Grants

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorCheung, ANYen_HK
dc.contributor.authorTsun, KLen_HK
dc.contributor.authorNg, KMen_HK
dc.contributor.authorSzeto, Een_HK
dc.contributor.authorSiu, MKen_HK
dc.contributor.authorWong, ESen_HK
dc.contributor.authorNgan, HYen_HK
dc.date.accessioned2010-12-23T08:38:26Z-
dc.date.available2010-12-23T08:38:26Z-
dc.date.issued2010en_HK
dc.identifier.citationModern Pathology, 2010, v. 23 n. 4, p. 559-566en_HK
dc.identifier.issn0893-3952en_HK
dc.identifier.urihttp://hdl.handle.net/10722/129524-
dc.description.abstractP63 and p73 are two homologues of the important tumor suppressor gene p53. In this study, we investigated p63 and p73 expression by immunocytochemistry using antibodies for TAp73 and p634A4 isoforms in 91 high-grade and 107 low-grade squamous intraepithelial lesions, 212 atypical squamous cells of undetermined significance, 9 squamous cell carcinomas and 63 normal samples from an Asian screening population together with 47 hospital samples of carcinomas. There was significant correlation between the TAp73 and p634A4 indices (P0.0001). Significantly, higher TAp73 and p634A4 indices were found in high-grade lesions or carcinoma when compared with atypical squamous cells and low-grade lesions (P0.0001). Among atypical squamous cells, p634A4 indices of cases that subsequently progressed to low-grade (P0.031) or high-grade lesions (P0.006) were significantly higher than those that did not. For atypical squamous cells positive for high-risk human papillomavirus (HPV) as detected by Digene (61%), cases with high p634A4 index were still more likely to have subsequent high-grade lesions detected (P0.016). Among low-grade lesions, significantly higher TAp73 (P0.038) was found in cases that subsequently progressed to high-grade lesions. There was significant correlation between presence of high-risk HPV and p634A4 index (P0.01). In summary, p63 and p73 immunocytochemistry are potential good markers for detection of carcinoma and high-grade lesions in cervical cytology samples and for triage management of women with atypical squamous cells and low-grade lesions. © 2010 USCAP, Inc. All rights reserved.en_HK
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/modpathol/en_HK
dc.relation.ispartofModern Pathologyen_HK
dc.subjectLiquid-based cervical cytologyen_HK
dc.subjectP63en_HK
dc.subjectP73en_HK
dc.subject.meshCarcinoma, Squamous Cell - diagnosis - genetics-
dc.subject.meshDNA-Binding Proteins - biosynthesis - genetics-
dc.subject.meshMembrane Proteins - biosynthesis - genetics-
dc.subject.meshNuclear Proteins - biosynthesis - genetics-
dc.subject.meshPrecancerous Conditions - diagnosis - genetics-
dc.titleP634A4 and TAp73 immunocytochemistry in liquid-based cervical cytologypotential biomarkers for diagnosis and progress prediction of cervical neoplasiaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0893-3952&volume=23&spage=559&epage=566&date=2010&atitle=P634A4+and+TAp73+immunocytochemistry+in+liquid-based+cervical+cytology:+potential+biomarkers+for+diagnosis+and+progress+prediction+of+cervical+neoplasia-
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.emailSiu, MK: mkysiu@hkucc.hku.hken_HK
dc.identifier.emailNgan, HY: hysngan@hkucc.hku.hken_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.identifier.authoritySiu, MK=rp00275en_HK
dc.identifier.authorityNgan, HY=rp00346en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1038/modpathol.2009.198en_HK
dc.identifier.pmid20081800en_HK
dc.identifier.scopuseid_2-s2.0-77950518293en_HK
dc.identifier.hkuros176804en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77950518293&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume23en_HK
dc.identifier.issue4en_HK
dc.identifier.spage559en_HK
dc.identifier.epage566en_HK
dc.identifier.isiWOS:000276197100008-
dc.publisher.placeUnited Kingdomen_HK
dc.relation.projectCervical cancer screening by enhanced cervical cytology-application of novel markers-
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK
dc.identifier.scopusauthoridTsun, KL=6506326338en_HK
dc.identifier.scopusauthoridNg, KM=7403178500en_HK
dc.identifier.scopusauthoridSzeto, E=6603826287en_HK
dc.identifier.scopusauthoridSiu, MK=24924018400en_HK
dc.identifier.scopusauthoridWong, ES=23101622300en_HK
dc.identifier.scopusauthoridNgan, HY=34571944100en_HK
dc.identifier.citeulike6553954-

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