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Article: P634A4 and TAp73 immunocytochemistry in liquid-based cervical cytologypotential biomarkers for diagnosis and progress prediction of cervical neoplasia
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TitleP634A4 and TAp73 immunocytochemistry in liquid-based cervical cytologypotential biomarkers for diagnosis and progress prediction of cervical neoplasia
 
AuthorsCheung, ANY1
Tsun, KL1
Ng, KM1
Szeto, E1
Siu, MK1
Wong, ES1
Ngan, HY1
 
KeywordsLiquid-based cervical cytology
P63
P73
 
Issue Date2010
 
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/modpathol/
 
CitationModern Pathology, 2010, v. 23 n. 4, p. 559-566 [How to Cite?]
DOI: http://dx.doi.org/10.1038/modpathol.2009.198
 
AbstractP63 and p73 are two homologues of the important tumor suppressor gene p53. In this study, we investigated p63 and p73 expression by immunocytochemistry using antibodies for TAp73 and p634A4 isoforms in 91 high-grade and 107 low-grade squamous intraepithelial lesions, 212 atypical squamous cells of undetermined significance, 9 squamous cell carcinomas and 63 normal samples from an Asian screening population together with 47 hospital samples of carcinomas. There was significant correlation between the TAp73 and p634A4 indices (P0.0001). Significantly, higher TAp73 and p634A4 indices were found in high-grade lesions or carcinoma when compared with atypical squamous cells and low-grade lesions (P0.0001). Among atypical squamous cells, p634A4 indices of cases that subsequently progressed to low-grade (P0.031) or high-grade lesions (P0.006) were significantly higher than those that did not. For atypical squamous cells positive for high-risk human papillomavirus (HPV) as detected by Digene (61%), cases with high p634A4 index were still more likely to have subsequent high-grade lesions detected (P0.016). Among low-grade lesions, significantly higher TAp73 (P0.038) was found in cases that subsequently progressed to high-grade lesions. There was significant correlation between presence of high-risk HPV and p634A4 index (P0.01). In summary, p63 and p73 immunocytochemistry are potential good markers for detection of carcinoma and high-grade lesions in cervical cytology samples and for triage management of women with atypical squamous cells and low-grade lesions. © 2010 USCAP, Inc. All rights reserved.
 
ISSN0893-3952
2013 Impact Factor: 6.364
 
DOIhttp://dx.doi.org/10.1038/modpathol.2009.198
 
ISI Accession Number IDWOS:000276197100008
Funding AgencyGrant Number
Research Fund for the Control of Infectious Diseases/Health and Health Services Fund06060642
SK Yee Medical Foundation
Funding Information:

This study was supported by the Research Fund for the Control of Infectious Diseases/Health and Health Services Fund (Project Number: 06060642). The high-risk HPV Hybrid Capture Test II was supported by the SK Yee Medical Foundation.

 
ReferencesReferences in Scopus
 
GrantsCervical cancer screening by enhanced cervical cytology-application of novel markers
 
DC FieldValue
dc.contributor.authorCheung, ANY
 
dc.contributor.authorTsun, KL
 
dc.contributor.authorNg, KM
 
dc.contributor.authorSzeto, E
 
dc.contributor.authorSiu, MK
 
dc.contributor.authorWong, ES
 
dc.contributor.authorNgan, HY
 
dc.date.accessioned2010-12-23T08:38:26Z
 
dc.date.available2010-12-23T08:38:26Z
 
dc.date.issued2010
 
dc.description.abstractP63 and p73 are two homologues of the important tumor suppressor gene p53. In this study, we investigated p63 and p73 expression by immunocytochemistry using antibodies for TAp73 and p634A4 isoforms in 91 high-grade and 107 low-grade squamous intraepithelial lesions, 212 atypical squamous cells of undetermined significance, 9 squamous cell carcinomas and 63 normal samples from an Asian screening population together with 47 hospital samples of carcinomas. There was significant correlation between the TAp73 and p634A4 indices (P0.0001). Significantly, higher TAp73 and p634A4 indices were found in high-grade lesions or carcinoma when compared with atypical squamous cells and low-grade lesions (P0.0001). Among atypical squamous cells, p634A4 indices of cases that subsequently progressed to low-grade (P0.031) or high-grade lesions (P0.006) were significantly higher than those that did not. For atypical squamous cells positive for high-risk human papillomavirus (HPV) as detected by Digene (61%), cases with high p634A4 index were still more likely to have subsequent high-grade lesions detected (P0.016). Among low-grade lesions, significantly higher TAp73 (P0.038) was found in cases that subsequently progressed to high-grade lesions. There was significant correlation between presence of high-risk HPV and p634A4 index (P0.01). In summary, p63 and p73 immunocytochemistry are potential good markers for detection of carcinoma and high-grade lesions in cervical cytology samples and for triage management of women with atypical squamous cells and low-grade lesions. © 2010 USCAP, Inc. All rights reserved.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationModern Pathology, 2010, v. 23 n. 4, p. 559-566 [How to Cite?]
DOI: http://dx.doi.org/10.1038/modpathol.2009.198
 
dc.identifier.citeulike6553954
 
dc.identifier.doihttp://dx.doi.org/10.1038/modpathol.2009.198
 
dc.identifier.epage566
 
dc.identifier.hkuros176804
 
dc.identifier.isiWOS:000276197100008
Funding AgencyGrant Number
Research Fund for the Control of Infectious Diseases/Health and Health Services Fund06060642
SK Yee Medical Foundation
Funding Information:

This study was supported by the Research Fund for the Control of Infectious Diseases/Health and Health Services Fund (Project Number: 06060642). The high-risk HPV Hybrid Capture Test II was supported by the SK Yee Medical Foundation.

 
dc.identifier.issn0893-3952
2013 Impact Factor: 6.364
 
dc.identifier.issue4
 
dc.identifier.openurl
 
dc.identifier.pmid20081800
 
dc.identifier.scopuseid_2-s2.0-77950518293
 
dc.identifier.spage559
 
dc.identifier.urihttp://hdl.handle.net/10722/129524
 
dc.identifier.volume23
 
dc.languageeng
 
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/modpathol/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofModern Pathology
 
dc.relation.projectCervical cancer screening by enhanced cervical cytology-application of novel markers
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshCarcinoma, Squamous Cell - diagnosis - genetics
 
dc.subject.meshDNA-Binding Proteins - biosynthesis - genetics
 
dc.subject.meshMembrane Proteins - biosynthesis - genetics
 
dc.subject.meshNuclear Proteins - biosynthesis - genetics
 
dc.subject.meshPrecancerous Conditions - diagnosis - genetics
 
dc.subjectLiquid-based cervical cytology
 
dc.subjectP63
 
dc.subjectP73
 
dc.titleP634A4 and TAp73 immunocytochemistry in liquid-based cervical cytologypotential biomarkers for diagnosis and progress prediction of cervical neoplasia
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong