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Article: Diffusion tensor imaging of liver fibrosis in an experimental model

TitleDiffusion tensor imaging of liver fibrosis in an experimental model
Authors
Keywordsapparent diffusion coefficient
CCl 4
diffusion tensor imaging
diffusion-weighted imaging
fractional anisotropy
liver cirrhosis
liver fibrosis
MRI
Issue Date2010
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/1053-1807/
Citation
Journal Of Magnetic Resonance Imaging, 2010, v. 32 n. 5, p. 1141-1148 How to Cite?
AbstractPurpose To characterize changes in diffusion properties of liver using diffusion tensor imaging (DTI) in an experimental model of liver fibrosis. Materials and Methods Liver fibrosis was induced in Sprague-Dawley rats (n = 12) by repetitive dosing of carbon tetrachloride (CCl 4). The animals were examined with a respiratory-gated single-shot spin-echo echo-planar DTI protocol at 7 T before, 2 weeks after, and 4 weeks after CCl 4 insult. Apparent diffusion coefficient (ADC), directional diffusivities (ADC // and ADC ⊥), and fractional anisotropy (FA) were measured. Liver histology was performed with hematoxylin-eosin staining and Masson's trichrome staining. Results Significant decrease (P < 0.01) in ADC was found at 2 weeks (0.86 ± 0.09 × 10 -3 mm 2/s) and 4 weeks (0.74 ± 0.09 × 10 -3 mm 2/s) following CCl 4 insult, as compared with that before insult (0.97 ± 0.08 × 10 -3 mm 2/s). Meanwhile, FA at 2 weeks (0.18 ± 0.03) after CCl 4 insult was significantly lower (P < 0.01) than that before insult (0.26 ± 0.05), and subsequently normalized at 4 weeks (0.26 ± 0.07) after the insult. Histology showed collagen deposition, presence of intracellular fat vacuoles, and cell necrosis/apoptosis in livers with CCl 4 insult. Conclusion DTI detected the progressive changes in water diffusivities and diffusion anisotropy of liver tissue in this liver fibrosis model. ADC and FA are potentially valuable in detecting liver fibrosis at early stages and monitoring its progression. Future human studies are warranted to further verify the applicability of DTI in characterizing liver fibrosis and to determine its role in clinical settings. © 2010 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/129381
ISSN
2015 Impact Factor: 3.25
2015 SCImago Journal Rankings: 1.683
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Grant CouncilGRF HKU7808/09M
Funding Information:

Contract grant sponsor: Hong Kong Grant Council: Contract grant number: GRF HKU7808/09M.

References

 

DC FieldValueLanguage
dc.contributor.authorCheung, JSen_HK
dc.contributor.authorFan, SJen_HK
dc.contributor.authorGao, DSen_HK
dc.contributor.authorChow, AMen_HK
dc.contributor.authorMan, Ken_HK
dc.contributor.authorWu, EXen_HK
dc.date.accessioned2010-12-23T08:36:35Z-
dc.date.available2010-12-23T08:36:35Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Magnetic Resonance Imaging, 2010, v. 32 n. 5, p. 1141-1148en_HK
dc.identifier.issn1053-1807en_HK
dc.identifier.urihttp://hdl.handle.net/10722/129381-
dc.description.abstractPurpose To characterize changes in diffusion properties of liver using diffusion tensor imaging (DTI) in an experimental model of liver fibrosis. Materials and Methods Liver fibrosis was induced in Sprague-Dawley rats (n = 12) by repetitive dosing of carbon tetrachloride (CCl 4). The animals were examined with a respiratory-gated single-shot spin-echo echo-planar DTI protocol at 7 T before, 2 weeks after, and 4 weeks after CCl 4 insult. Apparent diffusion coefficient (ADC), directional diffusivities (ADC // and ADC ⊥), and fractional anisotropy (FA) were measured. Liver histology was performed with hematoxylin-eosin staining and Masson's trichrome staining. Results Significant decrease (P < 0.01) in ADC was found at 2 weeks (0.86 ± 0.09 × 10 -3 mm 2/s) and 4 weeks (0.74 ± 0.09 × 10 -3 mm 2/s) following CCl 4 insult, as compared with that before insult (0.97 ± 0.08 × 10 -3 mm 2/s). Meanwhile, FA at 2 weeks (0.18 ± 0.03) after CCl 4 insult was significantly lower (P < 0.01) than that before insult (0.26 ± 0.05), and subsequently normalized at 4 weeks (0.26 ± 0.07) after the insult. Histology showed collagen deposition, presence of intracellular fat vacuoles, and cell necrosis/apoptosis in livers with CCl 4 insult. Conclusion DTI detected the progressive changes in water diffusivities and diffusion anisotropy of liver tissue in this liver fibrosis model. ADC and FA are potentially valuable in detecting liver fibrosis at early stages and monitoring its progression. Future human studies are warranted to further verify the applicability of DTI in characterizing liver fibrosis and to determine its role in clinical settings. © 2010 Wiley-Liss, Inc.en_HK
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/1053-1807/en_HK
dc.relation.ispartofJournal of Magnetic Resonance Imagingen_HK
dc.rightsJournal of Magnetic Resonance Imaging. Copyright © John Wiley & Sons, Inc.-
dc.rightsSpecial Statement for Preprint only Before publication: 'This is a preprint of an article accepted for publication in [The Journal of Pathology] Copyright © ([year]) ([Pathological Society of Great Britain and Ireland])'. After publication: the preprint notice should be amended to follows: 'This is a preprint of an article published in [include the complete citation information for the final version of the Contribution as published in the print edition of the Journal]' For Cochrane Library/ Cochrane Database of Systematic Reviews, add statement & acknowledgement : ‘This review is published as a Cochrane Review in the Cochrane Database of Systematic Reviews 20XX, Issue X. Cochrane Reviews are regularly updated as new evidence emerges and in response to comments and criticisms, and the Cochrane Database of Systematic Reviews should be consulted for the most recent version of the Review.’ Please include reference to the Review and hyperlink to the original version using the following format e.g. Authors. Title of Review. Cochrane Database of Systematic Reviews 20XX, Issue #. Art. No.: CD00XXXX. DOI: 10.1002/14651858.CD00XXXX (insert persistent link to the article by using the URL: http://dx.doi.org/10.1002/14651858.CD00XXXX) (This statement should refer to the most recent issue of the Cochrane Database of Systematic Reviews in which the Review published.)-
dc.subjectapparent diffusion coefficienten_HK
dc.subjectCCl 4en_HK
dc.subjectdiffusion tensor imagingen_HK
dc.subjectdiffusion-weighted imagingen_HK
dc.subjectfractional anisotropyen_HK
dc.subjectliver cirrhosisen_HK
dc.subjectliver fibrosisen_HK
dc.subjectMRIen_HK
dc.subject.meshAnimals-
dc.subject.meshCarbon Tetrachloride-
dc.subject.meshDiffusion Tensor Imaging-
dc.subject.meshLiver - pathology-
dc.subject.meshLiver Cirrhosis, Experimental - chemically induced - diagnosis-
dc.titleDiffusion tensor imaging of liver fibrosis in an experimental modelen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1053-1807&volume=32&issue=5&spage=1141&epage=1148&date=2010&atitle=Diffusion+tensor+imaging+of+liver+fibrosis+in+an+experimental+model-
dc.identifier.emailMan, K: kwanman@hkucc.hku.hken_HK
dc.identifier.emailWu, EX: ewu1@hkucc.hku.hken_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityWu, EX=rp00193en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jmri.22367en_HK
dc.identifier.pmid21031520-
dc.identifier.scopuseid_2-s2.0-78349302736en_HK
dc.identifier.hkuros183301en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-78349302736&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume32en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1141en_HK
dc.identifier.epage1148en_HK
dc.identifier.isiWOS:000284190200017-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCheung, JS=16174280400en_HK
dc.identifier.scopusauthoridFan, SJ=36514618100en_HK
dc.identifier.scopusauthoridGao, DS=36664395800en_HK
dc.identifier.scopusauthoridChow, AM=16174234200en_HK
dc.identifier.scopusauthoridMan, K=7101754072en_HK
dc.identifier.scopusauthoridWu, EX=7202128034en_HK

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