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Article: Aquaporin-4 autoantibodies in neuromyelitis optica spectrum disorders: Comparison between tissue-based and cell-based indirect immunofluorescence assays

TitleAquaporin-4 autoantibodies in neuromyelitis optica spectrum disorders: Comparison between tissue-based and cell-based indirect immunofluorescence assays
Authors
Issue Date2010
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.jneuroinflammation.com/home/
Citation
Journal Of Neuroinflammation, 2010, v. 7 How to Cite?
AbstractBackground: Neuromyelitis optica spectrum disorders (NMOSD) are severe central nervous system inflammatory demyelinating disorders (CNS IDD) characterized by monophasic or relapsing, longitudinally extensive transverse myelitis (LETM) and/or optic neuritis (ON). A significant proportion of NMOSD patients are seropositive for aquaporin-4 (AQP4) autoantibodies. We compared the AQP4 autoantibody detection rates of tissue-based indirect immunofluorescence assay (IIFA) and cell-based IIFA.Methods: Serum of Chinese CNS IDD patients were assayed for AQP4 autoantibodies by tissue-based IIFA using monkey cerebellum and cell-based IIFA using transfected HEK293 cells which express human AQP4 on their cell membranes.Results: In total, 128 CNS IDD patients were studied. We found that 78% of NMO patients were seropositive for AQP4 autoantibodies by cell-based IIFA versus 61% by tissue-based IFA (p = 0.250), 75% of patients having relapsing myelitis (RM) with LETM were seropositive by cell-based IIFA versus 50% by tissue-based IIFA (p = 0.250), and 33% of relapsing ON patients were seropositive by cell-based IIFA versus 22% by tissue-based IIFA (p = 1.000); however the differences were not statistically significant. All patients seropositive by tissue-based IIFA were also seropositive for AQP4 autoantibodies by cell-based IIFA. Among 29 NMOSD patients seropositive for AQP4 autoantibodies by cell-based IIFA, 20 (69%) were seropositive by tissue-based IIFA. The 9 patients seropositive by cell-based IIFA while seronegative by tissue-based IIFA had NMO (3), RM with LETM (3), a single attack of LETM (1), relapsing ON (1) and a single ON attack (1). Among 23 NMO or RM patients seropositive for AQP4 autoantibodies by cell-based IIFA, comparison between those seropositive (n = 17) and seronegative (n = 6) by tissue-based IIFA revealed no differences in clinical and neuroradiological characteristics between the two groups.Conclusion: Cell-based IIFA is slightly more sensitive than tissue-based IIFA in detection of AQP4 autoantibodies, which are highly specific for NMOSD. © 2010 Chan et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/129283
ISSN
2015 Impact Factor: 4.667
2015 SCImago Journal Rankings: 2.538
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Faculty of Medicine of the University of Hong Kong
Funding Information:

This study is supported by Seed Funding for Basic Research from the Faculty of Medicine of the University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorChan, KHen_HK
dc.contributor.authorKwan, JSCen_HK
dc.contributor.authorHo, PWLen_HK
dc.contributor.authorHo, JWMen_HK
dc.contributor.authorChu, ACYen_HK
dc.contributor.authorRamsden, DBen_HK
dc.date.accessioned2010-12-23T08:34:39Z-
dc.date.available2010-12-23T08:34:39Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Neuroinflammation, 2010, v. 7en_HK
dc.identifier.issn1742-2094en_HK
dc.identifier.urihttp://hdl.handle.net/10722/129283-
dc.description.abstractBackground: Neuromyelitis optica spectrum disorders (NMOSD) are severe central nervous system inflammatory demyelinating disorders (CNS IDD) characterized by monophasic or relapsing, longitudinally extensive transverse myelitis (LETM) and/or optic neuritis (ON). A significant proportion of NMOSD patients are seropositive for aquaporin-4 (AQP4) autoantibodies. We compared the AQP4 autoantibody detection rates of tissue-based indirect immunofluorescence assay (IIFA) and cell-based IIFA.Methods: Serum of Chinese CNS IDD patients were assayed for AQP4 autoantibodies by tissue-based IIFA using monkey cerebellum and cell-based IIFA using transfected HEK293 cells which express human AQP4 on their cell membranes.Results: In total, 128 CNS IDD patients were studied. We found that 78% of NMO patients were seropositive for AQP4 autoantibodies by cell-based IIFA versus 61% by tissue-based IFA (p = 0.250), 75% of patients having relapsing myelitis (RM) with LETM were seropositive by cell-based IIFA versus 50% by tissue-based IIFA (p = 0.250), and 33% of relapsing ON patients were seropositive by cell-based IIFA versus 22% by tissue-based IIFA (p = 1.000); however the differences were not statistically significant. All patients seropositive by tissue-based IIFA were also seropositive for AQP4 autoantibodies by cell-based IIFA. Among 29 NMOSD patients seropositive for AQP4 autoantibodies by cell-based IIFA, 20 (69%) were seropositive by tissue-based IIFA. The 9 patients seropositive by cell-based IIFA while seronegative by tissue-based IIFA had NMO (3), RM with LETM (3), a single attack of LETM (1), relapsing ON (1) and a single ON attack (1). Among 23 NMO or RM patients seropositive for AQP4 autoantibodies by cell-based IIFA, comparison between those seropositive (n = 17) and seronegative (n = 6) by tissue-based IIFA revealed no differences in clinical and neuroradiological characteristics between the two groups.Conclusion: Cell-based IIFA is slightly more sensitive than tissue-based IIFA in detection of AQP4 autoantibodies, which are highly specific for NMOSD. © 2010 Chan et al; licensee BioMed Central Ltd.en_HK
dc.languageengen_US
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.jneuroinflammation.com/home/en_HK
dc.relation.ispartofJournal of Neuroinflammationen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsJournal of Neuroinflammation. Copyright © BioMed Central Ltd.-
dc.subject.meshAdolescent-
dc.subject.meshAquaporin 4 - immunology-
dc.subject.meshAutoantibodies - analysis - immunology-
dc.subject.meshDemyelinating Autoimmune Diseases, CNS - immunology-
dc.subject.meshFluorescent Antibody Technique, Indirect - methods-
dc.titleAquaporin-4 autoantibodies in neuromyelitis optica spectrum disorders: Comparison between tissue-based and cell-based indirect immunofluorescence assaysen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1742-2094&volume=7 article no. 50&spage=&epage=&date=2010&atitle=Aquaporin-4+autoantibodies+in+neuromyelitis+optica+spectrum+disorders:+Comparison+between+tissue-based+and+cell-based+indirect+immunofluorescence+assays-
dc.identifier.emailHo, PWL: hwl2002@hku.hken_HK
dc.identifier.emailChu, ACY: bcccy@hkucc.hku.hken_HK
dc.identifier.authorityHo, PWL=rp00259en_HK
dc.identifier.authorityChu, ACY=rp00505en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1186/1742-2094-7-50en_HK
dc.identifier.pmid20822515-
dc.identifier.pmcidPMC2941752-
dc.identifier.scopuseid_2-s2.0-77956474797en_HK
dc.identifier.hkuros178577en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77956474797&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.eissn1742-2094-
dc.identifier.isiWOS:000282385900001-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChan, KH=7406034963en_HK
dc.identifier.scopusauthoridKwan, JSC=36479956300en_HK
dc.identifier.scopusauthoridHo, PWL=25027612100en_HK
dc.identifier.scopusauthoridHo, JWM=8685214100en_HK
dc.identifier.scopusauthoridChu, ACY=24343085700en_HK
dc.identifier.scopusauthoridRamsden, DB=7102612805en_HK
dc.identifier.citeulike7816569-

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